摘要
为获得潜在益生乳酸菌,为其实际应用提供理论基础。从实验室现存菌库中选取3株乳杆菌(Lactobacillus spp.)进行体外实验。首先对3株乳杆菌进行模拟胃肠液耐受,结果表明4 h耐受后,乳杆菌的存活率均达79%以上,其中D-乳杆菌在模拟胃肠液中的活菌对数均没有显著性差异(P>0.05),耐受性最稳定;然后利用自聚集性和疏水性筛选出D-乳杆菌的自聚集性和疏水性最高,并且3株乳杆菌疏水性极强,均达80%以上,表明3株乳杆菌具有良好的潜在黏附特性;最后评价3株乳杆菌对水产病原菌和腐败菌荧光假单胞菌(Pseudomonas fluorescens)、腐败希瓦氏菌(Shewanella putrefaciens)、波罗的海希瓦氏菌23(Shewanella baltica 23)、波罗的海希瓦氏菌73(Shewanella baltica73)、波罗的海希瓦氏菌100(Shewanella baltica 100)的抑菌效果,结果显示3株乳杆菌具有很好的抑菌效果,且对波罗的海希瓦氏菌100的抑菌效果最强,抑菌圈直径与其他4株指示菌有显著性差异(P<0.05)。综合实验结果表明,3株乳杆菌具有良好的模拟胃肠液耐受性和黏附肠道的潜力,其中D-乳杆菌的益生潜力相对较好,且3株乳杆菌能够很好抑制水产微生物的生长。
In order to obtain potential probiotic lactic acid bacteria, and provide a theoretical basis for the practical application of probiotics, three strains of Lactobacillus were selected from the existing laboratory bacteria for in vitro experiments. First, three strains were evaluated tolerance to simulated gastrointestinal stress. The results showed that the survival rate of Lactobacillus were more than 79% after 4 h. There was no significant difference in the logarithm of D-Lactobacillus(P>0.05) which the tolerability was the most stable;Then the self-aggregation and hydrophobicity were used to screen, the results indicated that three Lactobacilli had good potential adhesion which the self-aggregation and hydrophobicity of D-Lactobacillus were the highest, and the three strains were highly hydrophobicity, all of which were above 80%. Finally, three strains were evaluated bacteriostatic effect for aquatic pathogens and spoilage bacteria which included Pseudomonas fluorescens, Shewanella putrefaciens, Shewanella baltica 23, Shewanella baltica 73 and Shewanella baltica 100. There were a good antibacterial effect on aquatic pathogens and spoilage bacteria which the antibacterial effect of Shewanella baltica 100 were significantly higher than others. The comprehensive experimental results showed that Lactobacillus had the potential tolerance to simulated gastrointestinal stress, adhere to the intestinal tract which D-Lactobacillus is relatively good, and they also could inhibit the growth of aquatic microbes.
作者
孙群
易华西
张冬
梁晶晶
蔡玉勇
张兰威
SUN Qun;YI Huaxi;ZHANG Dong;LIANG Jingjing;CAI Yuyong;ZHANG Lanwei(College of Food Science and Engineering,Ocean University of China,Qingdao 266003;Qingdao Genyuan Biological Company,Qingdao 266109)
出处
《食品科技》
CAS
北大核心
2019年第12期1-5,共5页
Food Science and Technology
基金
科技部国家重点研发项目(2018YFC0311201)
山东省科技攻关项目(KJ2018BAE041).
关键词
乳杆菌
模拟胃肠液耐受
自聚集性
疏水性
抑菌
Lactobacillus
simulated gastrointestinal fluid tolerance
auto-aggregation
hydrophobicity
inhibition
