摘要
目的探讨泌尿生殖道组织作为艾滋病病毒(HIV)感染者/艾滋病(AIDS)病人(简称HIV/AIDS病人)HIV储存库的可能。方法 20例接受高效抗反转录病毒治疗(HAART)至少6个月,需要行包皮切除术或泌尿道/生殖道腔镜手术的HIV/AIDS病人,检测标本组织和外周血单个核细胞(PBMC)的HIV-1总脱氧核糖核酸(DNA)。结果 20例病人平均年龄(45.8±14.7)岁,接受HAART(6~36个月),平均(14.1±7.9)个月,外周血HIV-1核糖核酸(RNA)均低于检测下限20拷贝/mL,CD4^+T淋巴细胞(简称CD4细胞)计数平均(417.3±151.3)个/μL,CD4细胞/CD8^+T淋巴细胞(简称CD8细胞)(CD4/CD8细胞)比值平均(0.55±0.35),泌尿生殖道组织和PBMC中均能检测到HIV-1总DNA,载量分别为(85.9~89 300)拷贝/10~6个细胞(中位数1 034.12拷贝/10~6个细胞)及(6.83~321 000)拷贝/10~6个细胞(中位数550拷贝/10~6个细胞),两者呈正相关(r=0.53,P=0.018)。但泌尿生殖道组织HIV-1总DNA载量与CD4细胞计数及CD4/CD8细胞比值变化均无明显相关性(r=-0.002,P=0.99;r=0.12,P=0.61)。结论泌尿生殖道组织可能是HIV感染者的病毒储存库之一。
Objective To investigate the distribution and quantity of HIV-1 DNA in urogenital mucosal tissues of HIV/AIDS patients after HAART. Methods We recruited 20 cases of HIV/AIDS patients undergoing circumcision or endoscopic surgery of the urinary tract. The median age was 45.8. All patients received HAART for more than six months with the HIV-1 RNA in peripheral blood mononuclear cells(PBMC) under the detection limit(20 copies/ml). We detected HIV-1 DNA in mucosal tissues, using HIV-1 DNA quantitative kits. Results All patients receiving HAART for more than 6 months(mean, 14.1±7.9 m) were HIV DNA-positive with DNA viral loads 85.9-89300 copies/10~6 cells(median, 1034.12 copies/10~6 cells) in urinary tissues and 6.83-321000 copies/10~6 cells(median, 550 copies/10~6 cells) in PBMC respectively, which showed a remarkably positive correlation. However, there was no significant correlation between the total DNA load, CD4^+, and CD4^+/CD8^+(r =-0.002, P =0.99, r=0.12, P =0.61). Conclusion Urogenital mucosa is potentially a HIV reservoir.
作者
朱郇荣
范立新
周雄才
李宝金
杨柳平
卢斯汉
李锋
何耀祖
李凌华
ZHU Xunrong;FAN Lixin;ZHOU Xiongcai;LI Baojin;YANG Liuping;LU Sihan;LI Feng;HE Yaozu;LI Linghua(Infectious Disease Center,Guangzhou Eighth People’s Hospital,Guangzhou 510060,China)
出处
《中国艾滋病性病》
CAS
CSCD
北大核心
2020年第1期4-7,共4页
Chinese Journal of Aids & STD
基金
国家“十三五”科技重大专项课题(2018ZX10302103-002)
广东省医学科学技术研究基金项目(A2016462)
广东省省级科技计划项目(2016B020238002).
