摘要
脑心肌炎病毒(EMCV)是一种重要的人畜共患病病原,但其致病机制目前尚不明确,小窝蛋白-1(Caveolin-1)可介导多种病毒感染宿主细胞。为了探究Caveolin-1在EMCV感染宿主细胞中的作用,该实验检测了EMCV感染不同时间段He La细胞中Caveolin-1的表达量,对He La细胞和HEK-293细胞中Caveolin-1进行瞬时过表达实验、瞬时沉默实验和Caveolin-1依赖型内吞途径抑制实验,然后观察EMCV对He La细胞和HEK-293细胞的感染情况。结果发现, HeLa细胞中Caveolin-1表达量会随病毒感染时间的增加而升高;在Caveolin-1瞬时过表达实验结果中,病毒滴度、病毒拷贝数、病毒蛋白检测结果均显示,上调Caveolin-1促进EMCV感染宿主细胞, Caveolin-1瞬时沉默实验和Caveolin-1依赖型内吞途径抑制实验的结果则显示,下调Caveolin-1或抑制Caveolin-1依赖型内吞途径可抑制EMCV感染宿主细胞。上述现象提示, EMCV可通过Caveolin-1依赖型内吞途径感染宿主细胞。
EMCV(encephalomyocarditis virus) is an important zoonotic pathogen, but its pathogenesis is not clear yet. Caveolin-1 mediate various viruses to infect host cells. In order to explore the effect of Caveolin-1 in EMCV-infection in vitro, this study detected the expression of Caveolin-1 at different times of EMCV-infection. And conducted the test of transient overexpression, transient silencing and inhibiting Caveolin-1 endocytic pathway on HeLa cells and HEK-293 cells, then observed the effects of viral infection. The results showed that the expression of Caveolin-1 in HeLa cells increased during virus infection. In the results of Caveolin-1 transient overexpression test, virus titer, virus copies and virus protein detection results showed that EMCV infection was promoted, the results of Caveolin-1 transient silencing test and inhibition test showed that EMCV infection was inhibited. These findings suggest that the Caveolin-1 dependent endocytosis pathway promotes EMCV infected host cells.
作者
刘杨
赵克学
许淑娟
刘翊忠
李琼毅
LIU Yang;ZHAO Kexue;XU Shujuan;LIU Yizhong;LI Qiongyi(Key Bio-engineering and Technology Laboratory,Biomedical Research Center of the Northwest Minzu University,Lanzhou 730030,China;Life Science and Engineering College of Northwest Minzu University,Lanzhou 730030,China)
出处
《中国细胞生物学学报》
CAS
CSCD
2019年第12期2253-2262,共10页
Chinese Journal of Cell Biology
基金
国家自然科学基金(批准号:31702234、31460665)
中央专项研究生科研创新项目(批准号:Yxm2018146)
教育部“创新团队发展计划”(批准号:IRT_17R88)资助的课题.
关键词
小窝蛋白-1
脑心肌炎病毒
人宫颈癌细胞
人胚肾细胞
Caveolin-1
EMCV(encephalomyocarditis virus)
HeLa(human cervical carcinoma cell)
HEK-293(human embryonic kidney cells)
