摘要
目的通过观察实验动物脑组织形态学改变及神经行为改变,了解新生大鼠脑白质损伤后的神经系统自我修复潜能。方法将48只2-3日龄SD新生大鼠随机分为脑室周围白质软化(PVL)模型组(24只)和对照组(24只),模型组结扎右侧颈总动脉并缺氧2.5 h制备成PVL模型,对照组仅游离右侧颈总动脉但不结扎及缺氧,分别于建模后3 d、7 d和14 d观察并比较两组大鼠脑组织形态学改变及神经行为能力情况。结果病理切片可见模型组大鼠脑内凋亡细胞、白质结构疏松、神经元细胞凝固性坏死,符合PVL的病理改变。建模后3 d、7 d、14 d两组均可见神经细胞增殖,模型组建模14d增殖细胞数大于建模3 d(P<0.05),髓鞘数目及髓鞘厚度小于对照组(P<0.05)。建模后14 d对照组空间位置觉、学习记忆能力优于模型组(P<0.05),建模后14 d模型组大鼠寻找平台时间较建模后3 d缩短(P<0.05)。结论建立模型符合PVL模型,发生脑损伤后14 d,观察到大鼠脑白质损伤后神经系统存在一定的自我修复潜能。
Objective To understand the potential of nervous system self-repair after white matter injury in neonatal rats by observing histomorphology and neurobehavioral changes in experimental animals.Methods Forty-eight 2~3-day-old SD neonatal rats were randomly divided into periventricular leukomalacia(PVL)model group(n=24)and control group(n=24).The model group was induced by ligation of the right common carotid artery and hypoxia for 2.5 hours,while the control group only dissociated the right common carotid artery without ligation and hypoxia.The morphological changes of brain tissue and neurobehavioral ability of the two groups rats were observed and compared at 3 days,7 days and 14 days after modeling.Results The pathological sections showed that apoptotic cells,loose structure of white matter and coagulative necrosis of neurons could be seen in the brain of rats in the model group,which were consistent with the pathological changes of periventricular leukomalacia.The proliferation of nerve cells was observed in both groups at 3 days,7 days and 14 days after modeling.The number of proliferative cells in 14 days was higher than that in 3 days after modeling(P<0.05),and the number and thickness of myelin sheath in the model group were lower than those in the control group(P<0.05).14 days after modeling,the spatial position perception and learning and memory ability of the control group were better than those of the model group(P<0.05).The time of finding the platform in the model group 14 days after modeling was shorter than that of 3 days after modeling(P<0.05).Conclusion The establishment of the model accords with the model of periventricular leukomalacia.14 days after brain injury,it is observed that the nervous system has a certain potential for self-repair after white matter injury in rats.
作者
冯玉晴
朱艳萍
FENG Yuqing;ZHU Yanping(The First Affiliated Hospital Of Xinjiang Medical University,Urumqi 830011,China)
出处
《新疆医学》
2022年第1期1-6,共6页
Xinjiang Medical Journal
基金
新疆维吾尔自治区自然科学基金面上项目(项目编号:2016D01C271)
关键词
新生大鼠
脑白质损伤
修复
病理改变
神经行为能力
Neonatal Rats
Brain White Matter Injury
Repair
Pathological Changes
Neurobehavioral Ability
