摘要
目的:以α-葡萄糖苷酶和DPP-4作为降血糖研究靶点,筛选出艳山姜对其有抑制作用的活性部位。方法:对艳山姜的根、茎、叶三个部位采用系统溶剂提取法制备不同部位的提取物。测定不同提取物对α-葡萄糖苷酶和DPP-4体外酶活的影响。结果:大部分艳山姜提取部位对α-葡萄糖苷酶表现出抑制作用,半数抑制浓度(IC_(50))值在10μg/mL浓度水平左右,其中,根部的甲醇提取物显示出最大抑制效果,其IC_(50)值可达到0.813μg/mL。艳山姜对DPP-4也有一定抑制作用,大多数提取部位的IC_(50)值在100-500μg/mL之间,其中,茎的乙酸乙酯提取物显示出最大抑制效果,IC_(50)为157.201μg/mL。结论:艳山姜提取物对α-葡萄糖苷酶和DPP-4均有一定的抑制作用,这可能是其具有降糖作用的主要作用机制。可优先考虑从其根的甲醇提取物和茎的乙酸乙酯提取物中分离纯化出具有降血糖活性的先导化合物。
Objective:In this study,α-glucosidase and DPP-4 were selected as hypoglycemic targets to screen the active parts of Alpinia zerumbet(Pers.)Burtt.et Smith(AZ)with hypoglycemic effect.Method:The systematic solvent extraction method was used to extract the extracts from root,stem and leaf of AZ.The inhibitory effect of α-glucosidase and DPP-4 with AZ extracts were evaluated by extracellular test.Results:The majority of AZ extracts showed strong inhibition effect of α-glucosidase,and the IC_(50) values of most plant parts were around 10μg/mL concentration level.Among them,the methanol extract from the root presented the maximum inhibitory effect,and the half inhibitory concentration(IC_(50))was 0.813μg/mL.AZ extracts also has an inhibitory effect on DPP-4,and the IC_(50) value of most extracted parts was between100μg/mL to 500μg/mL.The ethyl acetate extract of the stem performed the largest inhibition effect,with 157.201μg/mL(IC_(50)).Conclusion:AZ has a beneficial impact on α-glucosidase and DPP-4 inhibition,which may be the mechanism of their hypoglycemic effect.In the further study,the methanol extract of AZ roots and the ethyl acetate extract of its stems should be given priority to separating and purifying the lead compounds with hypoglycemic activity.
作者
江圆圆
胡颖
凌兰鑫
张桂珍
黄春韵
廖家秀
苏健
Jiang Yuanyuan;Hu Ying;Ling Lanxing;Zhang Guizhen;Huang Chunyun;Liao Jiaxiu;Su Jian(Guangxi Scientific Research Center of Traditional Chinese Medicine,Guangxi University of Chinese Medicine,Naning 530200;Faculty of Pharmacy,Guangxi University of Chinese Medicine,Naning 530200)
出处
《壮瑶药研究》
2023年第1期148-151,313,共5页
Research of Zhuang and Yao Ethnic Medicine
基金
广西壮瑶药重点实验室(桂科基字[2014]32号)
壮瑶药协同创新中心(桂教科研[2013]20号)
广西壮族自治区民族药资源与应用工程研究中心(桂发改高技函[2020]2605号)
广西中药一流学科中药学(民族医药方向)项目(桂教科研[2018]12号)
广西高校中药药理重点实验室(Jl700304)
关键词
艳山姜提取物
Α-葡萄糖苷酶
DPP-4
降糖作用
活性部位
extracts of Alpinia zerumbet(Pers.)Burtt.et Smith
α-glucosidase
DPP-4
Hypoglycemic effect
Active parts
