摘要
内生真菌YJ-17菌株为从鸦胆子茎部获得的内生真菌,其在PDA平板对峙试验中对多种植物病原菌有抑制作用,说明YJ-17菌株可以产生抑菌代谢物,且具有较宽的抑菌谱。在本文研究采用提取、TLC薄层层析、硅胶柱层析,重结晶,结合生物活性追踪等手段分离、纯化其抑菌活性物质,并利用核磁共振、质谱等波谱技术对其抑菌成分进行鉴定,以确定YJ-17菌株抑菌成分的种类。
The endophytic fungus YJ-17 strain is an endophytic fungus obtained from the stem of Brucea javanica.It has inhibitory effects on various plant pathogens in PDA plate confrontation experiments,indicating that the YJ-17 strain can produce antibacterial metabolites and has a wide antibacterial spectrum.In this paper,the research uses extraction,TLC thin layer chromatography,silica gel column chromatography,recrystallization,combined with biological activity tracking and other means to isolate and purify its antibacterial substances,and uses NMR,mass spectrometry and other spectral techniques to identify its antibacterial ingredients,so as to determine the type of bacteriostatic ingredients of YJ-17 strain.
作者
梁子宁
陈龙
Liang Zining;Chen Long(Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine,Nanning 530200,Guangxi;Collaborative Innovation Center of Zhuang and Yao Ethnic Medicine,Nanning 530200,Guangxi;Guangxi Zhuang Autonomous Region Ethnic Medicine Resources and Application Engineering Research Center,Nanning 530200,Guangxi)
出处
《壮瑶药研究》
2023年第3期160-164,共5页
Research of Zhuang and Yao Ethnic Medicine
基金
广西科技厅面上项目:2020GXNSFAA259043
广西科技基地和人才专项(桂科AD21238031)
国家科技部外国专家项目(GXL20200233001)
关键词
鸦胆子
内生真菌
抑菌效果
成分鉴定
Brucea javanica
Endophytic fungi
Antibacterial effect
Component identification
