摘要
目的:研究针刺对慢性骨骼肌损伤模型大鼠血清LDH活性的作用及影响。方法:选取59只健康雄性SD大鼠为研究对象,从中随机抽出6只作为空白对照组,剩余53只为实验组,对实验组大鼠进行慢性骨骼肌损伤模型的制备,造模成功后随机抽取实验组和空白对照组大鼠各3只进行骨骼肌(股外侧肌)HE染色及血清LDH活性检测以判断是否造模成功,然后将剩余50只实验组大鼠随机分为模型对照组、自然恢复组、刮痧组、推拿组、针刺组,每组10只:模型对照组不做治疗,造模结束后处死取材;自然恢复组不做治疗,造模结束笼内自由活动7天后取材;刮痧组采用局部刮痧治疗;推拿组采用小指法、揉法治疗;针刺组采用普通针刺治疗,均治疗7d后取材。空白对照组不进行造模,笼内自由活动直至取材。采用ELISA法对血清中LDH活性进行检测。结果:干预后,针刺组、推拿组及刮痧组大鼠血清中LDH活性下降显著,均明显低于模型对照组、自然恢复组(P<0.05),其中针刺组大鼠血清LDH活性明显低于刮痧组和推拿组,而刮痧组与推拿组比较,差异无统计学意义(P>0.05)。结论:针刺、推拿、刮痧均可降低大鼠血清LDH活性,其中针刺影响明显;针刺、推拿、刮痧治疗慢性骨骼肌损伤均有疗效,针刺疗效最佳。
Objective:To study the effect of acupuncture on serum LDH activity in rats with chronic skeletal muscle injury.Methods:59 healthy male SD rats were selected as the research object,6 rats were randomly selected as the blank control group,and the remaining 53 rats were selected as the experimental group,After successful modeling,3 rats in the experimental group and 3 rats in the blank control group were randomly selected for skeletal muscle(lateral femoral muscle)he staining and serum LDH activity detection to judge whether the modeling was successful.Then the remaining 50 rats in the experimental group were randomly divided into model control group,natural recovery group,scrapping group,massage group and acupuncture group,with 10 rats in each group:model control group without treatment,At the end of modeling,the rats were killed and the materials were collected;In the natural recovery group,there was no treatment,and the materials were taken after 7 days of free movement in the cage;The scraping group was treated with local scraping;The massage group was treated with little finger massage and kneading;The patients in the acupuncture group were treated with ordinary acupuncture for 7 days.The blank control group did not make the model,and moved freely in the cage until the material was taken.LDH activity in serum was detected by ELISA.Results:After the intervention,LDH activity in serum of rats in acupuncture group,massage group and scraping group decreased significantly,which were significantly lower than those in model control group and natural recovery group(P<0.05).LDH activity in serum of rats in acupuncture group was significantly lower than that in scraping group and massage group,but there was no significant difference between scraping group and massage group(P>0.05).Conclusion:Acupuncture,massage and scraping can reduce the activity of serum LDH in rats;Acupuncture,massage and scraping are effective in the treatment of chronic skeletal muscle injury,and acupuncture has the best effect.
作者
马晓丽
张晓陶
杨桦
Ma Xiaoli;Zhang Xiaotao;Yang Hua(College of Traditional Chinese Medicine of Ningxia Medical University,Yinchuan 750004,China;The Third People's Hospital of Ningxia,Yinchuan 750021,China;Key Laboratory of Hui-Medicine Modernization Ministry of Education,Belong to Ningxia Medical University,Yinchuan 750004,China)
出处
《亚太传统医药》
2021年第8期23-26,共4页
Asia-Pacific Traditional Medicine
基金
宁夏医科大学回医药重点实验室开放项目(XY201712)
国家自然科学基金地区基金(81660818)
关键词
慢性骨骼肌损伤
针刺
推拿
刮痧
乳酸脱氢酶
Chronic Skeletal Muscle Injury
Acupuncture
Massage
Scraping
Lactate Dehydrogenase
