摘要
目的:采用高效液相色谱法建立川芎药材和饮片的指纹图谱,从而考察川芎炮制前后的质量传递信息,为川芎饮片质量控制及质量标准的建立提供依据。方法:液相色谱柱以十八烷基硅烷键合硅胶为填充剂,以甲醇(A)-0.1%磷酸水溶液(B)为流动相;梯度洗脱(0~10min,15%→30%A;10~20min,30%→50%A;20~40min,50%→70%A;40~45min,70%A;45~55min,70%→100%A;50~60min,100%A;60~65min,100%→15%A;65~70min,15%A)流速:0.8mL·min^(-1),检测波长为270nm,柱温:30℃,进样量:10μL,理论板数按阿魏酸峰计算应不低于2000。通过测定15批川芎药材和饮片的指纹图谱,并用中药色谱指纹图谱相似度评价系统2012版对两者相似度进行配对t检验比较分析。结果:15批川芎药材及饮片指纹图谱匹配结果,可匹配成功30个共有峰(饮片29个共有峰),经筛选可得8个共有峰。经过与对照品对比标定出1个色谱峰,分别为2号(阿魏酸)。川芎炮制前后指纹图谱基本一致(配对样本相关系数γ=0.9188,其均值差异检验t=1.896,df=14,P=0.0788)。结论:川芎炮制成饮片后未改变川芎原药材的化学成分,保留了川芎原药材的质量属性,川芎炮制前后其化学成分变化无统计学差异(P>0.05)。
Objective:To establish the fingerprint of Ligusticum chuanxiong Hort.and yinpian by HPLC,so as to investigate the quality transfer information of Ligusticum chuanxiong Hort.before and after processing,and provide a basis for the quality control of Ligusticum chuanxiong Hort.and the establishment of quality standards.Methods:The liquid chromatography column was filled with octadecylsilane bonded silica gel and methanol(A)-0.1%phosphoric acid aqueous solution(B)as the mobile phase.Gradient elution(0~10 min,15%→30%A;10~20 min,30%→50%A;20~40 min,50%→70%A;40~45 min,70%A;45~55 min,70%→100%A;50~60 min,100%A;60~65 min,100%→15%A;65~70 min,15%A)flow rate:0.8 mL·min^(-1),detection wavelength:270 nm,column temperature:30℃,injection quantity:10 L,in according to ferulic acid peak,theoretical plate number should not be less than 2000 calculated.The fingerprint of 15 batches of Ligusticum chuanxiong Hort.and yinpian was determined,and the similarity of the two samples was compared and analyzed by paired t-test with the similarity evaluation system 2012.Results:The results of fingerprint matching of 15 batches of rhizoma chuanxiong and decoction pieces showed that 30 common peaks(29 common peaks)were successfully matched,and 8 common peaks were obtained after screening.One chromatographic peak was identified by comparing with the control substance,which was No.2(ferulic acid).The fingerprints of Ligusticum chuanxiong Hort.before and after processing were basically the same(the correlation coefficient of paired samples was 0.9188,and the mean difference test was t=1.896,df=14,P=0.0788).Conclusion:The chemical composition of rhizoma chuanxiong was not changed after processed into yinpian,and the quality attribute of rhizoma chuanxiong was retained.There was no statistical difference in the chemical composition of rhizoma chuanxiong before and after processed(P>0.05).
作者
瞿昊宇
何群
谢梦洲
陈光宇
Qu Haoyu;He Qun;Xie Mengzhou;Chen Guangyu(School of Informatics,Hunan University of Chinese Medicine;Hunan Engineering Technology Research Center For Medicinal and Functional Food,Hunan University of Chinese Medicine;Provincial Key Laboratory of TCM Diagnostics,Hunan University of Chinese Medicine;The Key Laboratory of Cardiopulmonary Disease Syndrome Differentiation and Medicinal Diet Therapy in Chinese Medicine,Hunan University of Chinese Medicine;College of Traditional Chinese Medicine,Hunan University of Chinese Medicine,Changsha 410208,China)
出处
《亚太传统医药》
2021年第11期41-46,共6页
Asia-Pacific Traditional Medicine
基金
湖南省药食同源工程中心开放基金(2018YSTY02)
2020年度湖南省大学生创新创业训练计划(2612)
2020年度农产品加工研究所重点实验室开放课题项目(17)
关键词
川芎
指纹图谱
HPLC
质量传递
中药色谱指纹图谱相似度评价系统
配对T检验
Ligusticum chuanxiong Hort.
Fingerprints
HPLC
Quality Transfer
Similarity Evaluation System for Chromatographic Fingerprints of Traditional Chinese Medicine
Paired t Test
