摘要
Liposomes,as one of the most successful nanotherapeutics,have a major impact on many biomedical areas.In this study,we performed laser scanning confocal microscope(LSCM)and immunohistochemistry(IHC)assays to investigate the intra-tumor transport and antitumor mechanism of GE11 peptideconjugated active targeting liposomes(GE11-TLs)in SMMC7721 xenograft model.According to classification of individual cell types in high resolution images,biodistribution of macrophages,tumor cells,cells with high epidermal growth factor receptor(EGFR)expres sion and interstitial matrix in tumor microenvironment,in addition,their impacts on intra-tumor penetration of GE11-TLs were estimated.Type I collagen fibers and macrophage flooded in the whole SMMC7721 tumor xenografts.Tumor angiogenesis was of great heterogeneity from the periphery to the center region.However,the receptor-binding site barriers were supposed to be the leading cause of poor penetration of GE11-TLs.We anticipate these images can give a deep reconsideration for rational design of target nanoparticles for overcoming biological barriers to drag delivery.
Liposomes,as one of the most successful nanotherapeutics,have a major impact on many biomedical areas.In this study,we performed laser scanning confocal microscope(LSCM) and immunohistochemistry(IHC) assays to investigate the intra-tumor transport and antitumor mechanism of GE11 peptideconjugated active targeting liposomes(GE11-TLs) in SMMC7721 xenograft model.According to classification of individual cell types in high resolution images,biodistribution of macrophages,tumor cells,cells with high epidermal growth factor receptor(EGFR) expres sion and interstitial matrix in tumor microenvironment,in addition,their impacts on intra-tumor penetration of GE11-TLs were estimated.Type I collagen fibers and macrophage flooded in the whole SMMC7721 tumor xenografts.Tumor angiogenesis was of great heterogeneity from the periphery to the center region.However,the receptor-binding site barriers were supposed to be the leading cause of poor penetration of GE11-TLs.We anticipate these images can give a deep reconsideration for rational design of target nanoparticles for overcoming biological barriers to drag delivery.
基金
supported by National Science Foundation of China(Grant Nos.30825045 and 81273465).
