摘要
2019年12月,全球爆发新型冠状病毒肺炎疫情(COVID-19),使生物安全面临着巨大危机。病原微生物的快速、准确、低成本检测是保障生物安全的重要组成元素之一,是疫情预防、控制和诊断的关键。本综述介绍了核酸测序技术、等温扩增技术和基因编辑技术(CRISPR/Cas)于便携式一体化设备中的应用,为研发"低成本,高效率,结果可视化"集成式检测方法提供了可能性,旨在为病原微生物的有效防控提供保障。
In December 2019,the global outbreak of Corona Virus Disease 2019(COVID-19)made biosafety an attractive and crucial development direction globally.Rapid,accurate and low-cost detection of pathogenic microorganisms is one of the important issue to ensure biosafety,and is the key to epidemic prevention,control and diagnosis.This review elaborately introduces the applications of biosensors based on nucleic acid sequencing,isothermal amplification and gene editing(CRISPR/Cas)in integrated portable devices.The development of these technologies offers the possibility of developing"low-cost,high-efficiency and result-visualization"integrated detection methods.It is expected to provide technology guarantees for the effective prevention and control of pathogenic microorganisms.
作者
李子玥
杨同仁
杨歌
黄渊余
LI Zi-Yue;YANG Tong-Ren;YANG Ge;HUANG Yuan-Yu(School of Life Science,Advanced Research Institute of Multidisciplinary Science,Key Laboratory of Molecular Medicine and Biotherapy,Beijing Institute of Technology,Beijing 100081,China;School of Materials and the Environment,Beijing Institute of Technology,Zhuhai 519085,China)
出处
《应用化学》
CAS
CSCD
北大核心
2021年第5期592-604,共13页
Chinese Journal of Applied Chemistry
基金
国家自然科学基金(No.31871003)
北京市科技新星(No.Z201100006820005)
广东省自然科学基金(No.2019A1515010776)资助
