摘要
目的应用热脱附气相色谱-质谱联用(thermal desorption-gas chromatography-mass spectrometry,TD-GC-MS)技术对低剂量辐射损伤SD大鼠呼出气中挥发性有机化合物(volatile organic compounds,VOCs)成分进行探索,寻找大鼠辐射损伤后系列VOCs差异性代谢物,并为建立无创、非侵入性辐射损伤检测方法奠定基础。方法将SD大鼠随机分为5组(空白组、0.5 Gy组、1 Gy组、2 Gy组、3 Gy组),每组8只,建立大鼠低剂量辐射损伤模型。进行钴源辐射损伤后,记录大鼠体重,分析外周血血象,采集第1、5、9、13天大鼠呼出气,运用TD30-GC-MS技术分析呼出气中的VOCs成分,进行多元统计分析,探索并获得辐射损伤后的差异性代谢物。结果辐射损伤后大鼠与空白组相比,体重短期内下降、外周血象和肺组织切片具有差异性,大鼠呼出气中的VOCs成分含量与空白组大鼠具有明显差异,其中苯乙酮、壬醛、癸醛和十四烷4种VOCs增加,庚烷、氯苯、对二甲苯和间二氯苯4种VOCs减少。结论通过对大鼠呼出气的GC-MS分析,8种大鼠呼出气的VOCs成分可作为辐射损伤的差异性代谢物。此研究为建立大鼠呼出气VOCs成分的GC-MS分析方法,以及开展生物辐射损伤的无损伤性分析检测奠定了基础。
Objective To explore composition of volatile organic compounds(VOCs)in exhaled breath of low-dose radiation-damaged Sprague-Dawely(SD)rats by thermal desorption-gas chromatography-mass spectrometry(TD-GC-MS),and search for the differential metabolites of VOCs in the series of rats after radiation damage,and establish a noninvasive radiation damage detection method.Methods SD rats were randomly divided into five groups(a blank group,a 0.5-Gy group,a 1-Gy group,a 2-Gy group,and a 3-Gy group),with 8 rats in each group.A low-dose radiation injury model was established in rats.After the cobalt source radiation damage was performed,the body weight of rats was recorded,peripheral blood hematology was analyzed,and the exhaled breath of rats was collected on the 1^(st),5^(th),9^(th)and 13^(th)day.The composition of VOCs in the exhaled breath was analyzed by using the TD30-GC-MS technique,and multivariate statistical analyses were carried out to explore and obtain the differentiated metabolites after the radiation damage.Results After radiation damage,the rats showed a short-term decrease in body weight,peripheral blood and lung tissue sections were different,and the content of VOCs components in the exhaled breath of the damaged rats was significantly different from that of the rats in the blank group.Among them,four VOCs,acetophenone,nonanal,decanal and tetradecane increased,while heptane,chlorobenzene,paraxylene and m-dichlorobenzene decreased.Conclusions Through the GC-MS analysis of the exhaled breath of rats,eight components of VOCs in the exhaled breath of rats can be used as differential metabolites of radiation damage.This study lays a foundation for the establishment of a GC-MS analysis method for the components of VOCs in the exhaled breath of rats,as well as for the development of a nondestructive analytical assay for biological radiation damage.
作者
谢晓云
汤健
肖炳坤
缪潇瑶
李志恒
黄荣清
XIE Xiaoyun;TANG Jian;XIAO Bingkun;MIAO Xiaoyao;LI Zhiheng;HUANG Rongqing(Guangdong Pharmaceutical University,Guangzhou,Guangdong 510006,P.R.China;Institute of Radiational Chinese Medicine,Academy of Military Medical Sciences,Academy of Military Sciences,Beijing 100850,P.R.China)
出处
《中国呼吸与危重监护杂志》
CAS
CSCD
2024年第5期340-345,共6页
Chinese Journal of Respiratory and Critical Care Medicine
基金
北京市自然科学基金资助项目(7172161)
关键词
辐射损伤
呼出气
挥发性有机化合物
差异性代谢物
Radiation damage
exhaled breath
volatile organic compounds
differential metabolites
