摘要
为阐明闽西地区猪流行性腹泻病毒(PEDV)的遗传进化和重组事件。本研究从福建龙岩感染PEDV仔猪送检样品中鉴定出1株PEDV(闽西FILY1703株),采用PCR方法扩增其N、E、M以及S基因序列并测序。N、E、M以及S基因同源性及进化分析显示,FJLY1703株与CV777等早期经典株同源性较低且亲缘关系较远,而与2010年后国内分离株的同源性较高且进化关系较近。S基因编码的氨基酸序列与疫苗株CV777 S蛋白的氨基酸序列相比,FJLY1703株S蛋白抗原表位COE中存在8个突变,这些变化与2010年后中国PEDV分离株相似;且FJLY1703株E、M以及N基因编码的氨基酸序列中分别存在1个、3个和12个突变位点,均与我国当前流行株突变位点相似。S基因的重组分析表明,FJLY1703株是一株以CV777株为代表的早期经典株与2010年后PEDV变异株的重组流行株,上述研究结果提示在闽西地区需要研制针对PEDV流行株的疫苗来控制PEDV的感染。本研究为闽西地区PEDV流行病学研究提供参考依据。
In order to clarify the recombination and genetic evolution of porcine epidemic diarrhea virus(PEDV)isolates in western Fujian,a PEDV strain(FILY1703)was isolated from PEDV infected piglet samples in western Fujian,and its N,E,M and S genes were amplified by PCR and further sequenced and analyzed by bioinformatics software in this study.The homology and evolutionary analysis results showed that FJLY1703 had low homology and distant relationship with classical strains such as CV777,but high homology and close evolutionary relationship with the prevalent variants isolated in domestic after 2010.The amino acid sequence analysis showed the COE of FJLY1703 strain S protein had 8 mutations that were similar to prevalent variants in China after 2010 in comparison with the vaccine strain CV777.Further more,the amino acid sequence of FJLY1703 strain E,M and N genes had 1,3 and 12 mutation sites respectively,which were the same as the prevalent variants in China.Recombination analysis of S gene showed that FJLY1703 was a recombination strain of classical strain CV777 and mutant strain that prevalent variants after2010,and suggested the need to develop a vaccine against PEDV epidemic strains to control the infection of PEDV.This study provided data support for the epidemiological study of PEDV in China.
作者
董波
傅云扉
戴爱玲
李晓华
杨小燕
DONG Bo;FU Yun-fei;DAI Ai-ling;LI Xiao-hua;YANG Xiao-yan(College of Life Science of Longyan University,Longyan 364012;Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology,Longyan 364012,China)
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2020年第4期336-340,共5页
Chinese Journal of Preventive Veterinary Medicine
基金
福建省自然科学基金面上项目(2019J01804)
福建省高校自然基金青年重点项目(JZ160481)
福建省教育厅中青年科技项目(JAT160483)
关键词
猪流行性腹泻病毒
S基因
E基因
M基因
N基因
遗传进化分析
序列分析
重组分析
porcine epidemic diarrhea virus
S gene
E gene
M gene
N gene
genetic evolution analysis
sequence analysis
recombination analysis
