摘要
目的探讨人乳头瘤病毒(human papillomavirus,HPV)感染与喉癌组织中P16INK4a蛋白表达的相关性。方法选取2017年1~12月义乌市中心医院采集喉癌患者喉癌样本及相应癌旁组织样本,各60例。采用显色原位杂交法测定喉癌组织及癌旁组织中HPV16/18表达情况,采用HC-2法定量检测中HPV16/18病毒载量,采用免疫组化二步法测定喉癌组织及癌旁组织P16INK4a蛋白表达。结果喉癌组织中HPV16/18阳性表达率53.33%(32/60),高于癌旁组织0%(0/60),差异有统计学意义(χ~2=43.636,P<0.01)。喉癌组织中HPV16/18病毒载量阳性率68.33%(41/60),高于癌旁组织6.67%(4/60),差异有统计学意义(χ~2=48.676,P<0.01);喉癌组织中HPV16/18病毒载量(248.16±124.15)RLU/CO,高于癌旁组织(42.35±25.16)RLU/CO,差异有统计学意义(t=12.585,P<0.01)。喉癌组织中P16INK4a蛋白阳性率61.67%(37/60),高于癌旁组织10.00%(6/60),差异有统计学意义(χ~2=34.829,P<0.01)。喉癌组织中HPV16/18蛋白表达及HPV16/18病毒载量均与P16INK4a蛋白表达呈正相关(r=0.600,0.642,P<0.01)。T3-T4期HPV16/18阳性率61.11%(22/36),T1-T2期25.00%(6/24),差异有统计学意义(χ~2=7.545,P=0.006);T3-T4期HPV16/18病毒载量阳性率83.33%(30/36),T1-T2期45.83%(11/24),差异有统计学意义(χ~2=9.358,P=0.002)。有淋巴结转移HPV16/18阳性率71.43%(15/21),高于无淋巴结转移33.33%(13/39),差异有统计学意义(χ~2=7.959,P=0.005);有淋巴结转移HPV16/18病毒载量阳性率85.71%(18/21),高于无淋巴结转移58.97%(23/39),差异有统计学意义(χ~2=4.510,P=0.034)。结论喉癌组织中HPV16/18表达量、病毒载量及P16INK4a蛋白表达量升高,HPV16/18和P16INK4a可能相互作用,共同参与喉癌的发生与发展。
Objective To explore the association between human papillomavirus(HPV)infection and the expression of P16 INK4 a protein in laryngeal squamous cell carcinoma.Methods Laryngeal cancer tissue specimens and the corresponding para-tumor tissue specimens of 60 cases underwent surgery from January 2017 to December 2017 in Yiwu Central Hospital were collected,the expressions of HPV16/18 and HPV16/18 viral load were determined using color in situ hybridization and HC-2 assay.The expressions of P16 INK4 a protein in laryngeal carcinoma tissues and para-cancerous tissues were determined by immunohistochemistry.Results The positive expression rate of HPV16/18 in laryngeal cancer tissue was significantly higher than that in adjacent tissues(53.33%vs 0%,χ~2=43.636,P<0.01).The positive rate and mean value of HPV16/18 viral load in laryngeal cancer tissues were both higher than those in adjacent tissues(68.33%vs 6.67%,χ~2=48.676,P<0.01;248.16±124.15 vs 42.35±25.16 RLU/CO,t=12.585,P<0.01).The positive rate of P16 INK4 a protein in laryngeal cancer tissue was higher than that in adjacent tissues(61.67%vs 10.00%,χ~2=34.829,P<0.01).The expression of HPV16/18 protein and HPV16/18 viral load were positively correlated with the expression of P16 INK4 aprotein in laryngeal carcinoma(r=0.600,0.642,P<0.01).The positive rate of HPV16/18 and the positive rate of HPV16/18 viral load in T3-T4 stage were higher than those in T1-T2 stage(61.11%vs 25.00%,χ~2=7.545,P=0.006;83.33%vs 45.83%,χ~2=7.959,P=0.005).The positive rate of HPV16/18 and the positive rate of HPV16/18 viral load in lymph node metastasis were higher than those without(71.43%vs 33.33%,χ~2=7.959,P=0.005;85.71%vs 58.97%,χ~2=4.510,P=0.034),the differences were statistically significant.Conclusions The expression and viral load of HPV16/18 and the expression of P16 INK4 aprotein in laryngeal carcinoma are elevated.HPV16/18 and P16 INK4 amay interact and both involve in the development of laryngeal cancer.
作者
刘海芳
LIU Hai-fang(Department of Pathology,Yiwu Central Hospital,Yiwu,Zhejiang 322000,China)
出处
《中国预防医学杂志》
CAS
CSCD
2020年第4期393-396,共4页
Chinese Preventive Medicine
基金
浙江省医药卫生科技计划基金资助项目(2016KYB321)
