摘要
目的:探讨miR-361-5p对肾细胞癌ACHN细胞增殖、侵袭、迁移、凋亡及其细胞周期的影响。方法:将miR-361-5p mimics和miR-361-5p inhibitor分别转染至肾癌ACHN细胞中,用q PCR检测转染细胞中miR-361-5p的表达水平,用MTT法、划痕愈合实验、Transwell实验、流式细胞术分别检测细胞的增殖、迁移、侵袭、细胞周期和凋亡水平。结果:与空白对照组和Mimics-NC组比较,miR-361-5p mimics组ACHN细胞中miR-361-5p表达水平显著升高(P<0.01),细胞的增殖、侵袭和迁移能力均显著减弱(均P<0.01),而凋亡率升高(P<0.01)。与空白对照组或Inhibitor-NC组比较,miR-361-5p inhibitor组细胞中miR-361-5p表达水平显著下降(P<0.01),细胞的增殖、侵袭和迁移能力均增强(均P<0.01),细胞周期运转加速(P<0.01),凋亡率降低(P<0.05)。结论:miR-361-5p可抑制肾癌ACHN细胞的增殖、侵袭和迁移,并诱导细胞凋亡,其在肾癌发生发展过程中发挥重要的抑制作用。
Objective:To investigate the effect of miR-361-5 p on proliferation,invasion,migration,apoptosis and cell cycle of renal cell carcinoma ACHN cells.Methods:MiR-361-5 p mimics and miR-361-5 p inhibitor were transfected into ACHN cells,respectively.The expression of miR-361-5 p in the transfected cells was detected by Real-time quantitatibse PCR;and the proliferation,migration,invasion,cell cycle and apoptosis of cells were detected by MTT assay,Scratch-healing assay,Transwell assay and flow cytometry,respectively.Results:Compared with the control group and mimics-NC group,the expression of miR-361-5 p was increased significantly in ACHN cells of-miR-361-5 p mimics group(P<0.01),the abilities of cell proliferation,invasion and migration were decreased significantly(all P<0.01),and the apoptosis rate was increased(P<0.01).Compared with the control group or inhibitor-NC group,the expression of miR-361-5 p was significantly decreased in ACHN cells of miR-361-5 p inhibitor group(P<0.01),the abilities of cell proliferation,invasion and migration were increased(all P<0.01),the cell cycle was accelerated(P<0.01),and the apoptosis rate was decreased(P<0.05).Conclusion:miR-361-5 p can inhibit the proliferation,invasion and migration of renal cell carcinoma ACHN cells,and induce cell apoptosis,which plays an important inhibitory role in the development of renal cell carcinoma.
作者
余枫
谢亚平
叶莹
夏洪
YU Feng;XIE Yaping;YE Ying;XIA Hong(Department of Urology,Wuchang Hospital ofWuhan City,Wuhan 430063,Hubei,China)
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
北大核心
2020年第1期50-54,共5页
Chinese Journal of Cancer Biotherapy
