摘要
目的探究20种常见氨基酸在结直肠癌(CRC)患者的尿液特征;寻找CRC诊断的生物学标志物。方法纳入100名健康对照者和113名CRC患者(Ⅰ期27例,Ⅱ期28例,Ⅲ期34例,Ⅳ期24例),基于超高效液相色谱串联质谱法对尿液中的20种常见氨基酸应用多反应监测技术进行靶向定量分析,应用统计学方法分析CRC患者与健康对照氨基酸含量差异,并构建CRC诊断模型。结果CRC患者尿液中16种氨基酸含量与健康对照差异有统计学意义,Ⅰ,Ⅱ,Ⅲ和Ⅳ期各自有9种,15种,14种,17种氨基酸含量与健康对照差异有统计学意义。由异亮氨酸,天冬酰胺,脯氨酸,组氨酸,酪氨酸,苯丙氨酸6种氨基酸构建的生物标志物组合用于CRC诊断的AUC值在发现组/验证组分别为0.857/0.851,在Ⅰ,Ⅱ,Ⅲ和Ⅳ期CRC的AUC值分别为0.847,0.843,0.897,0.896。结论尿液中20种氨基酸含量变化可以用于CRC诊断,为CRC的早期诊断提供了新的研究思路。
Objective To investigate the urinary characteristics of 20 amino acids in patients with colorectal cancer(CRC);Constructing a biomarker panel for CRC diagnosis.Methods 100 healthy controls and 113 CRC patients were enrolled(27 in stageⅠ,28 in stageⅡ,34 in stageⅢand 24 in stageⅣ).Targeted quantitative analysis of 20 common amino acids in urine was performed using multiple reaction monitoring technology based on ultra-high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS).Statistical methods were used to analyze the differences in amino acids content between CRC patients and healthy controls,and a CRC diagnostic model was constructed.Results There was a statistical significance in the content of 16 amino acids in CRC urine compared to healthy controls,and 9,15,14,and 17 amino acids in StageⅠ,Ⅱ,ⅢandⅣshowed a statistical difference.A biomarker panel composed of six amino acids,isoleucine,asparagine,proline,histidine,tyrosine,and phenylalanine was constructed for CRC diagnosis.The AUC in the discovery/validation group were 0.857/0.851,and in the StageⅠ/Ⅱ/Ⅲ/Ⅳof CRC,the AUC value was 0.847/0.843/0.897/0.896,respectively.Conclusion Changes in the content of 20 amino acids in urine can be used for the diagnosis of CRC,and this study provides new research ideas for the diagnosis of CRC.
作者
祁峰
孙玉琳
刘佳琦
郭正光
刘晓燕
孙海丹
何成彦
赵晓航
孙伟
QI Feng;SUN Yulin;LIU Jiaqi;GUO Zhengguang;LIU Xiaoyan;SUN Haidan;HE Chengyan;ZHAO Xiaohang;SUN Wei(Department of Pharmacology,Institute of Basic Medicine Sciences CAMS,School of Basic Medicine PUMC,Beijing 100005,China;State Key Laboratory of Molecular Oncology,National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100021,China;Department of Clinical Laboratory,China-Japan Union Hospital of Jilin University,Changchun 130033,China)
出处
《中国实验诊断学》
2024年第4期395-401,共7页
Chinese Journal of Laboratory Diagnosis
基金
中国医学科学院医学科学创新基金(2021-I2M-1-016,2021-I2M-1-066,2022-I2M-1-020)
关键词
氨基酸
尿液
靶向分析
结直肠癌
amino acids
urine
targeted analysis
colorectal cancer
