摘要
目的了解深圳地区多囊卵巢综合征(PCOS)患者血清中25-羟基维生素D[25-(OH)VD]、抗苗勒管激素(AMH)水平及AMH和其Ⅱ型受体(AMHR II)基因多态性,并探讨其与PCOS发病之间的相关性。方法选取2019年1月至2020年7月在深圳市龙华区人民医院及各社康服务中心妇产科就诊并确诊为PCOS患者107例为PCOS组,并选择同期来医院检查的健康育龄妇女95例为对照组,分别检测血清中25-(OH)VD和AMH水平,同时采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)法对AMH G/T和AMHR II A/T基因多态性进行检测,并对检测结果进行统计分析。结果PCOS组患者血清中25-(OH)VD水平为32.97±6.41nmol/L,明显低于对照组的63.84±12.60nmol/L,而AMH水平为17.68±6.84ng/ml,明显高于对照组的5.28±1.97ng/ml,差异有统计学意义(P<0.05);PCOS组患者AMH G/T基因TT基因型和T等位基因检出率分别为39.26%和52.80%,明显高于对照组的28.42%和42.73%,差异均有统计学意义(P<0.05);PCOS组患者AMHRⅡA/T基因TT基因型及T等位基因检出率分别为22.43%和38.32%,明显高于对照组的11.58%和26.32%,差异有统计学意义(P<0.05);携带AMH基因TT基因型的PCOS患者血清中AMH水平为21.85±7.63ng/ml,明显高于AT基因型的14.96±5.42ng/ml,且AT基因型明显高于AA基因型的9.25±2.78ng/ml,而TT基因型的25-(OH)VD低于AT基因型,且AT基因型低于TT基因型,不同基因型PCOS患者血清中AMH、25-(OH)VD水平之间差异均有统计学意义(P<0.05);PCOS患者25-(OH)VD水平明显降低,而AMH水平明显升高,经Spearman相关性分析,结果显示两者呈负相关(r=-0.6275,P<0.05)。结论深圳地区PCOS患者血清中25-(OH)VD水平明显降低,而AMH水平明显升高,且两者呈负相关。同时PCOS患者AMH G/T基因TT基因型和AMHRⅡA/T基因TT基因型检出率明显升高,可能是该地区PCOS发病的遗传易感基因。
Objective:To understand the levels of 25-hydroxyitamin D[25(OH)VD],Anti-mullerian hormone(AMH)and the polymorphisms of AMH and itsⅡreceptor(AMHR II)gene in patients with polycystic ovary syndrome(PCOS)in serum in Shenzhen area,and discuss the correlation between it with PCOS.Methods:107 cases of PCOS patients admitted to the department of Obstetrics and Gynecology of Longhua District People’s Hospital and other social and health service centers from January 2019 to July 2020 were selected as the PCOS group,and 95 healthy women of childbearing age who came to the hospital for examination during the same period were selected as the control group,the levels of 25-(OH)VD and AMH in serum were detected,and the polymorphisms of AMH G/T and AMHR II A/T genes were detected by polymerase chain reaction-restricted fragment length polymorphism(PCR-RFLP),and the results were statistically analyzed.Results:The level of 25-(OH)VD in the PCOS group was 32.97±6.41nmol/L,which was significantly lower than 63.84±12.60nmol/L in the control group,and the level of AMH was 17.68±6.84ng/ml,which was significantly higher than 5.28±1.97ng/ml in the control group,the difference was statistically significant(P<0.05).The detection rates of AMH G/T TT genotype and T allele in PCOS group were 39.26%and 52.80%,respectively,which were significantly higher than 28.42%and 42.73%in the control group,with statistically significant differences(P<0.05).The detection rate of AMHRⅡA/T gene genotype TT and T allele were 22.43%and 38.32%respectively in PCOS patients,and significantly higher than 11.58%and 26.32%in the control group,the difference was statistically significant(P<0.05);the level of AMH in PCOS patients with AMH TT genotype was 21.85±7.63ng/ml,which was significantly higher than 14.96±5.42ng/ml of AT genotype,while AT genotype was significantly higher than 9.25±2.78ng/ml of AA genotype,However,25-(OH)VD of TT genotype was lower than that of AT genotype,and AT genotype was lower than that of TT genotype,and the levels of AMH and
作者
雷艾华
何广营
王宏亮
刘国栋
张燕
LEI Ai-hua;HE Guang-ying;WANG Hong-liang;LIU Guo-dong;ZHANGYan(Obstetrics and Gynecology,The People′s Hospital of Longhua District,Shenzhen,Guangdong Shenzhen 518109;Clinical laboratory,The People′s Hospital of Longhua District,Shenzhen,Guangdong Shenzhen 518109;Urology,The People′s Hospital of Longhua District,Shenzhen,Guangdong Shenzhen 518109)
出处
《中国优生与遗传杂志》
2020年第8期937-940,共4页
Chinese Journal of Birth Health & Heredity
