摘要
目的分析卵巢上皮癌患者血清微RNA(miR)-622、miR-944水平及临床意义。方法前瞻性选取2018年1月至2021年6月余姚市人民医院收治的70例卵巢上皮癌患者作为研究组,另选取同期50例卵巢良性肿瘤患者作为对照组。检测两组血清miR-622、miR-944水平及肿瘤组织E钙黏附蛋白(E-cadherin)、丝裂原活化蛋白激酶-1(MAPK1)、细胞外调节蛋白激酶(ERK)蛋白表达,分析血清miR-622、miR-944水平与肿瘤组织E-cadherin、MAPK1、ERK蛋白表达的相关性;绘制受试者工作特征(ROC)曲线,分析血清miR-622、miR-944诊断卵巢上皮癌的效能。结果研究组血清miR-622、miR-944水平及肿瘤组织E-cadherin、MAPK1、ERK表达高于对照组[14.72±1.87比5.94±1.26、13.02±1.65比4.15±0.62、(5.12±0.77)分比(2.37±0.34)分、(6.47±1.09)分比(2.56±0.45)分、(5.58±0.86)分比(1.87±0.28)分],差异有统计学意义(P<0.05);卵巢上皮癌患者血清miR-622、miR-944水平与E-cadherin、MAPK1、ERK表达均呈正相关(P<0.05);ROC曲线分析结果表明,血清miR-622、miR-944及联合诊断卵巢上皮癌的曲线下面积分别为0.763、0.797、0.808。结论卵巢上皮癌患者血清miR-622、miR-944水平升高,与肿瘤组织E-cadherin、MAPK1、ERK蛋白表达呈正相关。
Objective To analyze the expression and clinical significance of serum microRNA(miR)-622 and miR-944 in patients with epithelial ovarian cancer.Methods Seventy patients with ovarian epithelial cancer admitted in Yuyao People′s Hospital from January 2018 to June 2021 were selected as the study group,and 50 patients with benign ovarian tumors were selected as the control group.The expression of serum miR-622,miR-944 and the expression of tumor tissue protein E cadherin,mitogen-activated protein kinase-1(MAPK1),extracellular regulated protein kinases(ERK)were detected in both groups,and the correlation between miR-622,miR-944 and tumor tissue protein were analyzed.The receiver operating characteristic(ROC)curve was drawn to analyze the efficacy of miR-622 and miR-944 in the diagnosis of ovarian epithelial cancer.Results The levels of serum miR-622,miR-944 and the expression of E-cadherin,MAPK1 and ERK in the study group were higher than those in the control group:14.72±1.87 vs.5.94±1.26,13.02±1.65 vs.4.15±0.62,(5.12±0.77)scores vs.(2.37±0.34)scores,(6.47±1.09)scores vs.(2.56±0.45)scores,(5.58±0.86)scores vs.(1.87±0.28)scores,there were statistical differences(P<0.05).The serum levels of miR-622,miR-924 were positively correlated with the expression of E-cadherin,MAPK1 and ERK(P<0.05).The area under the curve of miR-622,miR-944 and combined diagnosis of ovarian epithelial cancer were 0.763,0.797,0.808,respectively.Conclusions The serum level of miR-622 and miR-944 were up-regulated in the patients with ovarian epithelial cancer,which was positively correlated with the expression of E-cadherin,MAPK1 and ERK protein in tumor tissues.
作者
邢士超
吴刚
侯树峰
朱银科
田炳如
Xing Shichao;Wu Gang;Hou Shufeng;Zhu Yinke;Tian bingru(Department of Oncology,Yuyao People′s Hospital,Yuyao 315400,China;Department of Tumor Radiotherapy,Affiliated Hospital of Jiangnan University,Wuxi 214100,China;Department of Clinical Laboratory,Yuyao People′s Hospital,Yuyao 3154000,China;Department of Pathology,Yuyao People′s Hospital,Yuyao 3154000,China)
出处
《中国医师进修杂志》
2023年第10期953-956,共4页
Chinese Journal of Postgraduates of Medicine
