摘要
目的:观察水通道蛋白1(Aquaporin 1,AQP1)、水通道蛋白2(Aquaporin 2,AQP2)、水通道蛋白3(Aquaporin 3,AQP3)在膀胱尿路上皮癌组织及正常膀胱组织中的表达与分布,探讨其意义。方法:取膀胱尿路上皮癌组织25例及正常膀胱组织25例,应用免疫组织化学方法检测AQP1、AQP2和AQP3在膀胱尿路上皮癌和正常膀胱组织中的表达及分布。结果:AQP1散在表达于正常膀胱组织中微血管和小动脉的内皮细胞的细胞膜及细胞质,在膀胱尿路上皮癌组织中AQP1大量表达于肿瘤的血管内皮细胞的细胞膜及细胞质;膀胱尿路上皮癌中AQP1表达水平较正常膀胱组织明显增高,差异有统计学意义(P<0.05);AQP2主要表达于膀胱尿路上皮癌组织及正常膀胱组织的细胞间质中,两者表达强度基本相同,差异无统计学意义(P>0.05);AQP3主要表达在膀胱尿路上皮癌组织及正常膀胱组织的黏膜上皮细胞的胞质及胞膜,在癌组织中的表达强度明显增高,差异有统计学意义(P<0.05)。结论:AQP1、AQP3在膀胱尿路上皮癌组织中表达明显增高。提示AQP1、AQP3可能与膀胱尿路上皮癌的发生和发展密切相关,其机制需要进一步深入研究。
Objective： To detect the expressions,distribution and the significances of aquaporin 1（AQP1）,aquaporin 2（AQP2）and aquaporin 3（AQP3）in normal bladder tissues and bladder tumor tissues.Methods：The expression and distribution of AQP1、AQP2 and AQP3 were detected by S-P immunohistochemistry in 25 cases of bladder tumor tissues and normal bladder tissues of 25 cases.Results：The AQP1was sporadically expressed in the vascular endothelial cells of normal bladder tissues.The majority expression of AQP1 could be detected in the vascular endothelial cells of tumor cells.The expression of AQP1 was remarkably higher in tumor tissues than in normal bladder tissues.Sporadic expression of AQP2 could be detected in intercellular substance in both bladder tumor tissues and normal bladder tissues.The expression of AQP2 in both tumor tissues and normal bladder tissues had no significant difference.the expression of AQP3 was found mainly in the celluar membrane and periplasm of the mucous membrane in both bladder tumor tissues and normal bladder tissues.The expression of AQP3 was significantly higher in tumor tissues than in normal bladder tissues.Conclusion：The expression of AQP1 and AQP3 were highter in bladder tumor tissues than those in normal bladder tissues.It suggested that AQP1 and AQP3 might related with the formation and development of bladder tumor.But the mechanism need to be further studied.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2012年第5期405-408,共4页
Journal of Chongqing Medical University
基金
新疆医科大学第一附属医院青年资助项目(编号:2009QN12)
