摘要
目的建立检测中药降压制剂中非法掺入的西药尼莫地平的专属性方法。方法采用Venusil MP C18色谱柱(150mm×4.6mm,5μm),以0.01mol/L醋酸铵(用冰醋酸调pH至3.5)-乙腈(30∶70)为流动相,对中药降压制剂的提取液进行液相色谱-质谱(LC-MS)联用分析。通过与对照品的色谱、紫外及质谱行为比较,对中药降压制剂中非法掺入的合成降压药进行定量测定和定性鉴别。结果在中药降压制剂中检出了尼莫地平,且质量浓度在8～80μg/mL范围内与峰面积线性良好(r=0.9994),平均回收率为98.78%,RSD=0.39%(n=6)。结论LC-MS联用法选择性强,灵敏度高,能分析检测该类中药降压制剂中的西药降压成分。
Objective To develop a specific method for identifying nimodipine added in traditional Chinese medicine. Methods The liquid chromatography-mass spectrometry （LC-MS）method was used. The Venusil MP Cl8 column was used with 0. 01 mol/L ammonium acetate solution（adjusted to pH3.5 with acetic acid）-acetonitrile（30：70） as the mobile phase. The extract of traditional Chinese antihypertensive preparations was analyzed. Nimedipine illegally added into the traditional Chinese medicine was qualitatively identified and determined quantitatively according to the spectrum chromatographic behavior and mass spectral data by comparison with those of reference substances. Results Nimodipine was found in the traditional Chinese medicine. The good linearity existed in the range of 8- 80 μg/mL for nimodipine. The average recovery rate was 98.78% （n =6）. Conclusion The method is well selective and highly sensitive, which can be used to detect the western medicine antihypertensive ingredient added illegally into traditional Chinese medicine antihypertensive preparations.
出处
《中国药业》
CAS
2009年第19期28-29,共2页
China Pharmaceuticals
关键词
尼莫地平
液相色谱-质谱联用法
定量测定
定性鉴别
nimodipine
liquid chromatography-mass spectrometry method
qualitative determination
qualitative identification
