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新型冠状病毒核酸检测方法 被引量:27

Methods for nucleic acid detection of severe acute respiratory syndrome coronavirus 2
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摘要 从2019年12月开始识别和救治新型冠状病毒(SARS-CoV-2)感染的肺炎至今,新型冠状病毒肺炎的确诊病例数已超过70000例,快速、准确地诊断对于SARS-CoV-2感染的控制至关重要。目前已知的用于人类流感病毒感染的诊断方法主要有:病毒的分离、特异性抗原检测以及核酸扩增等,核酸扩增所需窗口期短、耗时短,因此在病毒的早期、快速诊断中有一定的优势。SARS-CoV-2为单链RNA病毒,针对RNA病毒的较成熟的核酸诊断技术主要有:实时荧光定量逆转录-聚合酶链反应、逆转录环介导等温扩增、依赖核酸序列扩增、重组酶聚合酶扩增技术和基因芯片等。本文对这些常用的核酸诊断技术的基本原理和在SARS-CoV-2检测中的应用现状进行了综述,为目前抗击疫情的临床一线提供一些有用参考。 Begin to identify and treat pneumonia infected by the severe acute respiratory sydrome-coronvirus-2(SARS-CoV-2)from December 2019.So far,the number of confirmed cases of corona virus disease 2019 has exceeded 70,000,and rapid and accurate diagnosis is essential for the control of SARS-CoV-2 infection.The currently known diagnostic methods for human influenza virus infection mainly include:virus isolation,specific antigen detection,nucleic acid amplification,etc.The nucleic acid amplification requires a short window period and time-consuming,so there are certain advantages in the early rapid and diagnosis.The SARS-CoV-2 is a single-stranded RNA virus.The more mature nucleic acid diagnostic techniques for RNA viruses mainly include:real-time fluorescent quantitative reverse transcription-polymerase chain reaction(RT-PCR),reverse transcription loop-mediated isothermal amplification(RT-LAMP),nucleic acid sequence amplification(NASBA),recombinase polymerase amplification technology(RPA)and gene chips.This article reviews the basic principles of these commonly used nucleic acid diagnostic technologies and their application status in the detection of SARS-CoV-2,and provides some useful references for the current frontline clinical fight against the epidemic.
作者 许金和 王水良 张胜行 赖国祥 兰小鹏 吴小丽(综述) 余宗阳(审校) XU Jinhe;WANG Shuiliang;ZHANG Shenghang;LAI Guoxiang;LAN Xiaopeng;WU Xiaoli;YU Zongyang(Fuzhou General Hospital of Fujian Medical University,Fuzhou,Fujian 350000,China;Department of Clinical Laboratory,900 Hospital of Joint Logistic Team Support Force,Fuzhou,Fujian 350025,China;Department of Respiratory,900 Hospital of Joint Logistic Team Support Force,Fuzhou,Fujian 350025,China;Department of Medical Oncology,900 Hospital of Joint Logistic Team Support Force,Fuzhou,Fujian 350025,China)
出处 《国际检验医学杂志》 CAS 2020年第17期2138-2142,共5页 International Journal of Laboratory Medicine
关键词 新型冠状病毒 逆转录环介导等温扩增 等温核酸扩增 CRISPR/Cas 基因芯片 severe acute respiratory sydrome-coronvirus-2 reverse transcription loop-mediated isothermal amplification isothermal nucleic acid amplification CRISPR/Cas gene chip
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