摘要
目的研究云南省彝族人群t-PA基因Alu重复序列插入/缺失(I/D)多态性分布情况,为探讨t-PA基因相关性疾病的发病机制奠定基础。方法采用PCR-RFLP技术对云南省晋宁县275例彝族健康个体的t-PA基因Alu重复序列插入/缺失(I/D)多态性进行分析,计算II、ID、DD基因型频率和I、D等位基因频率,与其他人群进行比较。结果云南省晋宁县彝族人群t-PA基因的II、ID、DD基因型频率分别为24.36%、48.36%、27.27%,I和D等位基因频率分别为48.55%、51.45%。与报道的巴基斯坦人、印度人、韩国人、日本人和美国白种人的人群相比较,基因型分布和等位基因频率均无统计学差异(P>0.05)。与英国白种人人群相比较,无论基因型分布还是等位基因频率均差异有统计学意义(P<0.05)。结论t-PA基因Alu重复序列I/D多态性的分布在不同种族之间存在差异。
Objective To investigate the distribution of the t-PA Gene Alu-repeat I/D polymorphism in a Yi population of Yunnan province and to lay the foundation for exploring the pathogenesis of t-PA gene related diseases.Methods We studied 275 healthy individuals of Yi nationality in a rural area of Jinning,Yunnan province.The t-PAGene was identified by polymerase chain reaction-restriction fragment(PCR-RFLP)technique.The frequencies of II、ID、DD genotypes and I、D alleles were calculated.We compared the calculated results with other people.Results In the healthy Yi nationality population of Yunnan province,t-PA genotype frequencies for the II,ID and DD genotypes were 24.36%,48,36%,27.27%.Deriving from those,the allele frequencies for the I and D allele were confirmed which were 48.55%and 51.45%respectively.There were no significant differences in genotype distribution and allele frequencies between the reported Pakistani,Indian,Korean,Japanese,and American Caucasian populations(P>0.05).However,compared with the British white population,both the genotype distribution and the allele frequency were statistically significant(P<0.05).Conclusion In summary,the distribution of the Alu repeat I/D polymorphism of the t-PA gene differs among different ethnic groups.
作者
王乐
范志祥
陈志彬
彭红瑜
张延洁
张璐
万瑞雪
龙莉
WANG Le;FAN Zhi-xiang;CHEN Zhi-bin;PENG Hong-yu;ZHANG Yan-jie;ZHANG Lu;WAN Rui-xue;LONG Li(Dept.of Cell Biology and Medical Genetics,Kunming Medical University,Kunming Yunnan 650500;Dept.of Reproductive Genetics,the First Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650031,China)
出处
《昆明医科大学学报》
CAS
2020年第2期7-11,共5页
Journal of Kunming Medical University
基金
国家自然科学基金资助项目(81160164)
云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目[2018FE001(-188)]
昆明医科大学研究生创新基金资助项目(2019S067)。
