摘要
目的分析1型艾滋病病毒(HIV-1)抗体用蛋白印迹试验(WB)确证为阳性标本的带型,了解涪陵辖区HIV感染者的带型分布特征,为制定该地区切实有效的艾滋病防控措施提供科学依据。方法对2010-2018年涪陵辖区1872份WB确证为HIV-1抗体阳性标本的带型结果用SPSS 24.0软件统计分析。结果在1872份HIV-1抗体确证阳性标本中,10条带(全带)占36.5%(684份),7条带以上占93.2%(1745份);各WB带型的检出率:抗env(外膜蛋白)最高,gp160、gp120均为100%(1872份)、gp41为98.6%(1846份),其次是抗pol(反转录酶蛋白),p66为97.0%(1815份)、p51为94.1%(1762份)、p31为89.7%(1679份),抗gag(核心蛋白)除p24为99.3%(1858份)和p17为81.9%(1534份)外,其他带型检出率都较低,p55为44.9%(840份)、p39为53.5%(1002份)。感染者男女比为3.1∶1。不同性别比较p55、p39检出率的差异均有统计学意义;不同年龄比较p55、p31、p39、p17检出率的差异均有统计学意义;不同人群比较p66、p55、p39、p17检出率的差异均有统计学意义;不同感染途径比较p55、p39、p17检出率的差异均有统计学意义。结论涪陵辖区HIV-1抗体WB确证阳性标本带型检出率与其他地区的报道有差异,其原因有待进一步研究和探讨。
Objective To analyze the western blot(WB)band patterns in HIV-1 positive people in Fuling district,Chongqing,and to provide scientific evidence for prevention and control.Methods A total of 1872 samples with HIV-1 antibody positive from 2010 to 2018 in our laboratory were collected,and the SPSS 24.0 software was used for statistical analysis.Results In 1872 samples,36.5%(684)had ten bands,93.2%(1745)had more than 7 bands.The detection rate of WB band patterns was anti-env(envelope protein),the highest,such as gp160100%(1872),gp120100%(1872),and gp4198.6%(1846);followed by anti-pol(reverse transcriptase protein),such as p6697.0%(1815),p5194.1%(1762),and p3189.7%(1679).Of the anti-gag(core protein),except for p2499.3%(1858)and p1781.9%(1534),the other bands detection rate was low,such as p5544.9%(840),and p3953.5%(1002).The ratio of infected men to women was 3.1∶1.There were statistically significant differences in the detection rate of p55 and p39 between different genders,in the detection rate of p55,p31,p39,and p17 between different ages,in the detection rate of p66,p55,p39,and p17 between groups,and in the detection rate of p55,p39,and p17 between different infection ways.Conclusion There are differences in detection of HIV-1 antibody western blot bands in Fuling,compared with those reported in other areas,which is worth further research and discussion.
作者
康晓丽
刘乔磊
余天莉
艾彦彪
YU Ticuili;AI Yanbiao(Fuling Center for Disease Control and Prevention,Fuling 408000,Chongqing,China)
出处
《中国艾滋病性病》
CAS
CSCD
北大核心
2019年第10期1002-1005,共4页
Chinese Journal of Aids & STD
基金
重庆市涪陵区科技计划项目(FLKW,2017ABB1051)~~
