摘要
目的探讨HR-HPV持续感染引起的宫颈局部免疫微环境变化及其与宫颈病变程度、病毒亚型之间的关系。方法选取2015年11月至2016年4月就诊于天津市中心医院阴道镜门诊的105例HR-HPV持续感染者,按宫颈活检病理分为三组：NSIL、LSIL、HSIL各35例。用流式检测阴道灌洗液中IL-2、IL-4、IL-6、IL-10、TNF、IFN-γ、IL-17A水平,用Elisa方法检测灌洗液中TGF-β水平。同时收集所有研究对象的宫颈脱落细胞用焦磷酸测序对HR-HPV进行分型。结果随着宫颈病变程度的加重,IL-2浓度逐渐降低（P〈0.05）,IL-6浓度逐渐升高（P〈0.05）,其余6种因子在各组间差异均无统计学意义（P〉0.05）。HPV16为持续感染最常见的亚型（50.48%）,但三组间HPV型别分布差异无统计学意义（χ~2=8.02,P=0.091）。结论 HR-HPV持续感染引起宫颈病变的发生与局部免疫微环境密切相关,持续感染的HPV亚型与宫颈病变程度无关。
Objective To investigate the changes of cervical local microenvironment in high risk human papillomavirus（HR-HPV）persistent infection women and its relationship with cervical lesions and subtype of HPV.Methods 105 HR-HPV persistent infection patients were involved in this research and divided into 3 groups according to their cervical biopsy pathological results ：（1） No squamous intraepithelial lesion（NSIL） 35 cases.（2） Low grade squamous intraepithelial lesion（LSIL） 35 cases.（3） High grade squamous intraepithelial lesion（HSIL） 35 cases.Interleukin-2（IL-2）,IL-4,IL-6,IL-10,interferon-γ（IFN-γ）,tumor necrosis factor（TNF） and IL-17 A were detected by BD CBA,and transforming growth factor（TGF-β） was detected by Elisa.Cervical exfoliated cells were collected for HPV typing by nested PCR and pyrosequencing.Results The level of IL-2 decreased significantly with the severity of disease（P 〈0.05）,while the level of IL-6 inceased（P〈 0.05）.In all patients,HPV 16 was the most common genotype（50.48%）.But there was no significant difference of type distribution among the three groups（χ~2=8.02,P = 0.091）.Conclusion The occurrence of cervical lesions was closely related to the local immune microenvironment,but there was no relationship between HPV subtypes and cervical lesions.
出处
《中国妇产科临床杂志》
CSCD
北大核心
2018年第1期6-8,共3页
Chinese Journal of Clinical Obstetrics and Gynecology
基金
天津市卫生行业重点攻关项目(15KG140)
天津市科技计划项目(09ZCZDSF03900)
关键词
人乳头瘤病毒
细胞因子
免疫微环境
子宫颈上皮内瘤变
human papillomavirus
cytokine
immune microenvironment
cervical intraepithelial neoplasia
