摘要
甜瓜坏死斑点病毒(Melon necrotic spot virus,MNSV)是我国甜瓜病毒新纪录种,主要靠种子和土壤真菌传播。本文以MNSV山东分离物(MNSV-Shandong)为材料,利用RT-PCR和RACE技术获得了MNSV山东分离物基因组,并分析了其基因组成和进化关系。MNSV-Shandong基因组为4 267 nt,编码5个ORF,参与复制的p29和p89基因分别编码29 kDa和89 kDa蛋白质;p42基因编码的外壳蛋白为42 kDa;p7A和p7B基因编码的移动蛋白均为7 kDa。系统进化树表明MNSV-Shandong与海门分离物(MNSV-HM)聚在一起,与欧洲、部分亚洲及北美洲在一个分支上,与日本分离物距离较远。本文为国内首次报道MNSV山东分离物基因组。
The full-length nucleotide sequence and genome organization of a Melon necrotic spot virus isolated from Shandong,China（MNSV-Shandong）,were described. The MNSV-Shandong isolate consists of a positive sense single-stranded RNA with 4 267 nt in length. MNSV contains at least five open reading frames（ORFs） encoding p29,p89,p42 and two small p7 A and p7 B proteins,respectively. The p89 gene shares a common start codon with that of p29 and was generated through read-through of the amber stop codon of p29. Phylogenetic analyses based on complete genomic sequence assigned MNSV-Shandong and MNSV-HMChina isolates into a major cluster,MNSV-Shandong isolate and Europe,parts of Asia and North America isolates were in the same branch,but was far from the Japanese isolate. To the best of our knowledge,this is the first report of the genome of a MNSV Shandong isolate in China.
出处
《植物病理学报》
CAS
CSCD
北大核心
2017年第2期234-239,共6页
Acta Phytopathologica Sinica
基金
中国农业科学院创新团队西甜瓜病虫害防控(CAAS-ASTIP-2016-ZFRI)
国家西甜瓜产业技术体系病毒病害防控岗位(CARS-26-13)
