摘要
本文研究了偶氮胂 - 与钙的显色反应 ,在 p H=8.5氯化铵 -氨水缓冲液介质中 ,乳化剂 - OP存在下 ,偶氮胂 - 与钙反应生成 1∶ 1稳定络合物 ,λmax=680 nm,ε=2 .13× 10 4 L·mol-1·cm-1。钙含量在 0— 30μg/ 2 5m L范围内符合比耳定律 ,该方法用于烟草样品中钙含量的测定 。
Color reaction of calcium(Ⅱ) with arsenazo Ⅲ was studied.In the presence of pH=8.5 buffer solution and emulgent OP medium arsenazo Ⅲ can react with calcium(Ⅱ) to form a stable 1∶1 complex.The molar absorptivity is 2.13×10 4L·mol -1 ·cm -1 at 680nm.Beer's law is obeyed in the range of 0—30μg/25mL.This method can be applied to the determination of calcium in tobacco with satisfactory results.
出处
《光谱实验室》
CAS
CSCD
2001年第3期332-334,共3页
Chinese Journal of Spectroscopy Laboratory
