摘要
高密度发酵已经成为提高毕赤酵母目的蛋白表达量的关键技术之一,而其中发酵工艺又是高密度发酵的一个重要因素。主要从巴斯德毕赤酵母遗传学特性、表达宿主菌、表达载体、外源蛋白的表达方面进行了阐述,并从毕赤酵母工程菌的选择、培养基的优化设计、发酵过程关键参数调控以及甲醇诱导等方面阐述毕赤酵母的高密度发酵。最后,提出了巴斯德毕赤酵母高密度发酵过程中存在的问题并进行展望。
High-density fermentation has been a key technique tache to improve the expression level of the protein, and fermentation process is an important factor in high-density fermentation. The Pichia pastoris protein expression on its genetic characteristics, host strains, expression vectors and the expression of heterologous proteins in the areas of basic researches were reviewed. In addition, we briefly diseussed high-density fermentation of Pichia pastoris in the selection of engineering bacteria, designation and optimization of culture medium, control of the key biochemical parameters of fermentation process, and methanol induction. In the end, the problems existing in industry high-density fermentation progress were put forward, and the development trend was prospected.
出处
《生物技术通报》
CAS
CSCD
北大核心
2014年第3期42-49,共8页
Biotechnology Bulletin
基金
国家高技术研究发展计划("863"计划)(2012AA021201)
关键词
巴斯德毕赤酵母
表达系统
高密度发酵
过程参数
甲醇
Pichia pastoris
Expression system
High-density fermentation
Process parameters
Methanol
