摘要
目的:优选艾纳香中总黄酮的提取工艺。方法:采用紫外分光光度法测定总黄酮,以提取率为考察指标,用正交设计方法优选最佳提取工艺。结果:以总黄酮提取率为考察指标,其优选工艺为A3B2C1D3,即药材加30倍量的50%乙醇,80℃水浴回流提取1 h。结论:艾纳香中总黄酮的最佳提取工艺为药材加30倍量的50%乙醇,80℃水浴回流提取1 h。利用此工艺,艾纳香总黄酮提取率为17.48%。
Objective ： To optimize the extraction technology of total flavonoids in Blumea balsamifera Dc. Methods ： The content of total flavonoids was-determined by UV. The optimal extraction condition was investigated by orthogonal design and the extraction quantity was regarded as the investigated index. Results：The optimal extracting condition was A3 B2 C1 D3 with the extraction quantity of total flavonoids as the investigated index,adding thirty times amount of 50% ethanol, 1.0 hour of reflux at 80℃. Conclusion：The optimal extraction technology is adding thirty times amount of 50% ethanol, 1.0 hour of reflux at 80℃ of total flavonoids in Blumea balsamifera Dc. Flavonoids yield could reach 17.48% when using this extraction technology.
出处
《中华中医药学刊》
CAS
2013年第12期2735-2737,共3页
Chinese Archives of Traditional Chinese Medicine
基金
贵州省科技厅创新人才团队建设项目(黔科合人才团队20104006)
贵州省科技计划项目(20136011)
贵州大学人才引进项目(贵大人基合字2010013)
关键词
艾纳香
总黄酮
提取工艺
正交设计
Blumea balsamifera Dc
total flavonoids
extraction technology
orthogonal design
