摘要
目的了解2009—2010年山东省临沂市肠道病毒71型(EV71)分离株的基因特征,初步探讨VPl区核苷酸或氨基酸变异与不同临床类型的关系。方法从手足口病(HFMD)病例、重症病例和死亡病例的粪便标本中分离获得23株EV71病毒株,RT-PCR扩增VP1区,并进行序列测定和分析。结果 23株分离株与A、B基因型同源性较低;与C4亚型的C4a群代表株的核苷酸和氨基酸同源性分别达到92.8%～93.5%和98.3%～99.3%,明显高于其他亚型代表株的同源性;各分离株间的氨基酸序列比对显示无特征性改变。结论 2009—2010年临沂地区流行的EV71是C4亚型的C4a群,HFMD病例、重症病例和死亡病例分离株VP1区核苷酸序列和氨基酸序列无特征性差异。
Objective To explore the genetic characteristics of VP1 gene of enterovirus 71 strains isolated in Linyi,Shandong province,2009-2010 and to analyze the relationship between variations of VP1 gene and different clinical types.Methods The VP1 genes of 23 enterovirus strains isolated from stool and throat swab specimens of hadn-foot-and mouth disease（HFMD） cases were amplified with reverse transcription-PCR（RT-PCR） and their sequences were analyzed with DNASTAR 6 and MEGA 4.Results The homology between the 23 strains and the reference strains of subtype A and B was relatively low.The homologies of nucleotide and the amino acid of the 23 strains with the reference strains of group C4a were 92.8%-93.5% and 98.3%-99.3%,respectively,indicating that there was a close relationship between them.Comparisons of amino acid sequences among the 23 strains showed that there were no characteristic variations.Conclusion The enterovirus 71 strains isolated in Linyi,Shandong province belonged to the group C4a of subtype C4,and there were no characteristic variations in nucleotides or amino acids among HFMD cases,severe cases or death cases.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2012年第11期1446-1448,共3页
Chinese Journal of Public Health
基金
国家自然科学基金(30901265)
教育部博士点基金新教师基金(20090131120068)
山东省自然科学基金(ZR2010CQ030)
山东大学公共卫生学院青年人才创新基金(GGWS200801)
