摘要
本研究旨在检测内源性绵羊肺腺瘤反转录病毒(enJSRV)与干扰素-τ(IFN-τ)在妊娠早期蒙古绵羊子宫内膜组织的表达以及enJSRV的表达与外周血孕酮水平变化的关系。运用TaqMan实时荧光定量PCR技术和电化学发光法对enJSRV和IFN-τ在妊娠早期蒙古绵羊子宫内膜组织相对表达及孕酮水平进行了测定。实时荧光定量PCR结果显示,enJSRV和IFN-τmRNA在妊娠早期绵羊子宫内膜组织有不同程度的表达。SAS统计学软件分析得出,子宫内膜组织中enJSRV mRNA在妊娠12～14d(交配日为0d)表达较高,2～10、16～30d的表达均低于前者。IFN-τmRNA仅在妊娠12～25d表达,14d达其峰值,30d就不能检出,且差异都极显著(P<0.01)。电化学发光法结果显示,孕酮水平在妊娠2d为0.4ng·mL-1,以后升高,妊娠8～16d维持在8.3ng·mL-1左右,在妊娠19～30d孕酮水平有所下降,30d为2.5ng·mL-1。以上结果提示,子宫内膜组织中enJSRV mRNA的表达与外周血孕酮含量及IFN-τ的变化高度相关,且enJSRV在胎盘的形态发生及生殖生物学方面发挥重要作用。
In order to detect the expression of enJSRV and IFN-τ in endometrial tissue of Mongoli-an sheep as well as relationship of the expression of enJSRV and the variety of progesterone con-tent in peripheral blood during early pregnancy. TaqMan Real-time Quantify PCR was performedon the relative expression of enJSRV and IFN-τ in uterine endometrium, as well as progesteronecontent in peripheral blood was detected by electrochemiluminescence analyses. The results dem-onstrated that enJSRV and IFN-τ mRNA were expressed specifically in uterine endometrium ofMongolian sheep. The mRNA expression level of enJSRV was high on Day 12-14, while lowerthan the former on Day 2-10 and 16-30 during pregnancy. IFN-τ mRNA was expressed on Day 12-25 of gestation with the maximum value on Day 14, and IFN-τ cannot be detected on Day 30.Electrochemiluminescence results showed the progesterone level was 0.4 ng · mL^-1 on Day 2 androse afterwards on Day 8 of gestation, then kept at about 8.3 ng · mL^-1 on Day 8-16 and de-creased during Day 19-30 with the value 2.5 ng · mL^-1 on Day 30. These results indicated thatthe expression of enJSRV mRNA in endometrium was highly correlated with changes in peripher-al blood progesterone content and IFN-τ. Furthermore, enJSRV plays a significant role duringplacental morphogenesis and reproductive biology.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2012年第6期979-984,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金(31160493
30960271)
内蒙古科技创新引导基金项目(20101808)
教育部博士点基金博导类项目(20111515110008)
