摘要
将黑腹果蝇(Drosophila melanogaster)抗真菌肽基因Drosomycin(Drs)克隆到pPICZα-A载体中,构建分泌型表达载体pPICZα-A-Drs,转化宿主菌Pichia pastoris X-33.在AOX1(醇氧化酶)启动子调控下,抗真菌肽DRS成功表达,其分子量约为5 kD.抑菌试验显示,DRS对供试真菌有明显的抑菌活性.采用考马斯亮蓝法测定抗真菌肽的具体表达量,并优化了诱导条件.
The Drosophila melanogaster antifungal peptide gene Drosomycin （Drs）, was expressed in Pichia pastoris X-33 transformed by the recombinant expression vector pPICZα-A-Drs, which was combined by vector pPICZα-A and Drs. This resulting vector pPICZα-A-Drs contained α-Factor under the control of the alcohol oxidase 1 promoter. Following induction with methanol,the DRS peptide was synthesized with a molecular weight of 5.0 kD in a soluble form. Antifungal assay showed that the DRS had a obvious inhibitive effect on tested fungi. The amount of expression product DRS were determined by the coomassie blue staining,and the conditions of the expression were optimized.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第2期371-376,共6页
Journal of Sichuan University(Natural Science Edition)
基金
四川省科技攻关项目(07KJT20-20)
关键词
果蝇
抗真菌肽
分泌表达
毕赤酵母
Drosophila melanogaster, antifungal peptide, secretive expression, Pichia pastoris
