摘要
探讨中药何首乌饮对雌性衰老大鼠肾皮质bcl-2和bax表达的影响.选用50只雌性SD大鼠随机分为5组,即阴性对照组、模型组及何首乌饮高、中、低剂量组,每组10只.D半乳糖连续腹腔注射制作亚急性衰老大鼠模型.采用SP免疫组化法分别检测肾皮质组织中bcl-2和bax的表达情况.结果显示:1)模型组大鼠肾皮质bcl-2表达低于阴性对照组(P<0.05),何首乌饮高剂量组与模型组比较表达增强(P<0.05).2)模型组大鼠肾皮质bax表达明显高于阴性对照组(P<0.05),何首乌饮各剂量组与模型组比较表达减弱(P<0.05).研究提示何首乌饮可以通过上调bcl-2和下调bax表达,抑制肾皮质细胞凋亡,从而起到一定的延缓衰老作用.
To observe the effects of traditional Chinese medicine Heshouwuyin on the expression of bcl-2 and bax in renal cortex of aging rats. 50 female SD rats were applied in this study and randomised into 5 groups： negative control group, aging model group, Heshouwuyin groups（high, middle and low dose group）, 10 rats per group. The sub-acute aging model rats were made by injecting D-gal into abdominal cavity continuously. Using SP immunohistochemistry to observe the expression of bcl-2 and bax in renal cortex. Results： 1）The expression of bcl-2 in model groups was weaker than that of negative control group （P 〈 0.05 ）, the bcl-2 expression was stronger in Heshouwuyin high dose group than that of model group （P 〈 0.05）. 2） The expression of bax in model group was stronger than that of negative control group （P 〈 0. 05）. The expression of bax in Hes- houwuyin groups was weaker than that of model group （P 〈 0.05）. Traditional Chinese medicine Heshouwuyin could increase bcl-2 expression and decrease bax expression in renal cortex of aging rats obviously,and had fairly anti-aging effects.
出处
《河北师范大学学报（自然科学版）》
CAS
北大核心
2009年第4期521-524,共4页
Journal of Hebei Normal University：Natural Science
基金
国家自然科学基金(30572432)
