摘要
建立卷柏HPLC含量测定方法。采用Angilent C18色谱柱;流动相：甲醇-0.05mol·L-1磷酸二氢钠溶液（65∶35）;检测波长：330nm;柱温：35℃;流速：1mL·min-1;穗花杉双黄酮的进样量在0.3~1.97μg范围内线性良好（r=0.9999）,平均加样回收率98.2%,RSD=0.5%。本方法简便,准确,可靠,可为卷柏药材的质量标准的修订提供科学依据。
The method was established for the determination of Amentoflavone in Herba Selaginellae. The samples were determined by Agilent C18. The mobile phase was consisted of Methanol-0.05mol · L-1 Sodium Dihydrogen Phosphate （65 ： 35） ; The detection wavelength was 330nm. The calibration linear range of Amentoflavone was 0.3- 1.97μg （r= 0. 9999）, and the average recovery was 98.2%, RSD= 0. 5%. The method is reliable, accuracy and suitable for the quality evaluation of Herba Selaginellae.
出处
《中国药事》
CAS
2008年第8期661-663,共3页
Chinese Pharmaceutical Affairs
基金
<中国药典>2010年版中药材标准起草课题(编号YS-244)
