摘要
目的建立一种简易的牙槽骨缺损动物模型,为牙槽骨缺损正畸治疗的相关实验研究提供条件。方法选取Wistar大鼠40只,6周龄,体重200～300g,雌性,SPF级。麻醉下,去除一侧上颌第一磨牙近中区部分牙槽骨,后用倍骼生(Bio-glass)充填。术后2周,制作大鼠牙槽骨缺损修复侧的组织学切片,进行光学显微镜观察。结果40只大鼠中,1只死亡。存活大鼠手术切口愈合良好,术后2周,牙槽骨缺损修复区倍骼生颗粒周围有新骨形成的骨桥连接。结论以手术方法建立的牙槽骨缺损动物模型,可为今后的相关动物实验研究提供参考。
PURPOSE： To establish an animal model for experimental study of orthodontic treatment of alveolar bone defect and other relative research. METHODS： The experiment was carried out on forty SPF female Wistar rats,which were 6 weeks old. With the rats anaesthetized, one side of the alveolar bone in the maxillary first molar of the rats was removed partly. The defects were filled with Bio-glass. The tissue slices from the defects were observed under light microscope 2 weeks after surgery. RESULTS： Only one of the forty rats was infected after operation, and the survival rate was 97.5%. The wounds of the survived rats healed well, and new bone was found around the Bio-glass 2 weeks after operation. CONCLUSION： Animal model of alveolar bone defect established by surgery is simple and reliable, which can be used for further studies. Supported by National Natural Science Foundation of Shandong Province （Grant No.Y2004C13） and Medical Science and Technology Enhancement Award Project of Shangdong Province （Grant No.2005H2038）.
出处
《上海口腔医学》
CAS
CSCD
2006年第6期645-648,共4页
Shanghai Journal of Stomatology
基金
山东省自然科学基金(Y2004C13)
山东省医药卫生科技发展计划资助项目(2005HZ038)
关键词
牙槽骨缺损
动物模型
大鼠
Alveolar bone defect
Animal model
Rats
