摘要
羽毛山海绵(My ca le p lum ose)来源的糖多孢菌(S accharop oly sp ora sp.nov SP 2-10)具有诱导肿瘤细胞坏死的活性。本文对其发酵产物的活性部位乙酸乙酯层进行活性追踪分离,共得到7个化合物;利用理化性质和波谱学方法鉴定它们的结构分别为胆甾醇、邻苯二甲酸二异丁酯、4(2,4-二羟基苯甲酰氨基)苯甲酸、苯丙胺酸、脱氧鸟苷、鸟苷和N-乙酰酪胺;并初步评价上述化合物的抗肿瘤活性,结果表明,邻苯二甲酸二异丁酯显示细胞毒活性。
To explore the antitumor active components in ethyl acetate extract of Mycale plumose sponge-derived actinomycete Saccharopolyspora sp. nov by using solvent extraction, silica gel column, PHPLC and etc, seven compounds were isolated and purified. The separation procedure was guided by a bioassay using tsFT210 cell lines. By means of physico-chemical properties and spectral analysis, their structures were determined as ; cholesterine, 1,2-benzendicarboxylic acid bis （2-methylpropyl） ester, 4- （2,4-dihydroxybenzamido） benzoic acid, phenylalanine, deoxyguanosine, guanosine, N-acetyltyramine. The antitumor activity against tsFT210 cell line of these compounds was assayed and 1,2-benzendicar-boxylic acid bis （2-methylpropyl） ester showed significant cytotoxic activity.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2006年第11期646-649,共4页
Chinese Journal of Antibiotics
基金
国家863计划(2003A624020)
国家自然科学基金(No.30472136&30470196)
关键词
海绵共附生放线菌
羽毛山海绵
糖多孢菌
次级代谢产物
抗肿瘤活性
Songe associated-actinomycete
Mycale plurnose
Saccharopolyspora sp. nov
Secondary metabolites
Antitumor activity
