摘要
目的观察海水浸泡对烫伤大鼠创面炎性反应及愈合的影响。方法将144只雄性Wistar大鼠随机分为烫伤对照组和海水浸泡组,每组72只,均造成背部10%TBSA浅Ⅱ度烫伤。海水浸泡组大鼠伤后固定四肢,立即用盛海水的方盆浸泡双前肢以下部分,持续4h；烫伤对照组大鼠则用空方盆模拟浸泡过程。于伤后0(即刻,下同)、6、12、24h采用电解质分析仪测定血清中K^+、Na^+、Cl^-的浓度。于伤前及伤后0、6、12h采用酶联免疫吸附测定法检测血清中肿瘤坏死因子(TNF)α及白细胞介索(IL)6的含量。对两组大鼠创面行大体和组织病理学观察,并记录创面愈合时间。结果海水浸泡组大鼠血清中K^+、Na^+、Cl^-的浓度大多高于烫伤对照组。伤后6h海水浸泡组大鼠血清TNF-α、IL-6含量分别为(140±22)、(160±41)ng/L,均明显高于伤前值(29±15)、(62±17)ng/L及烫伤对照组(120±12)、(124±22)ng/L(P＜0．05)。与烫伤对照组比较,海水浸泡组大鼠创面水肿及局部组织炎性反应加重,创面再上皮化和表皮各层的分化延迟；海水浸泡组创面愈合时间为(16．3±1．6)d,明显迟于烫伤对照组(14．1±1．8)d(P＜0．05)。结论大鼠烫伤后经海水浸泡,可加重创面炎性反应,使创面愈合延迟。
Objective To investigate the influence of sea water immersion on inflammation and healing of the wounds in scalded rats. Methods One hundred and forty-four male Wistar rats with 10% TBSA superficial partial-thickness scald were randomly dividecd into A （ n = 72, with scald） and B （ n = 72, with seawater immersion for 4hrs immediately after scald）groups. The serum contents of K^±、Na^±、Cl^- were determined at 0 post-scald hour（PSH） , 6PSH, 12PSH and 24 PSH with electrocyte analysis apparatus, and the changes in serum interleukin-6 （IL-6） and tumor necrosis factor （TNF-α） levels were determined at 0 PSH, 6PSH and 12 PSH with enzyme-linked immunosorbent assay （ELISA）. The histopathological changes in the rats of the two groups were observed, and wound healing time was respectively calculated. Results The serum contents of K^±、Na^±、Cl^- in B group were obviously higher than those in A group. And the serum content of TNF-α and IL-6 in B group at 6 PSH [ （ 140 ± 22） ng/L , （ 160 ± 41 ） ng/L] were significantly higher than those before scald [ （29 ± 15 ） ng/L, （ 62 ± 17 ） ] ng/L and in A group [ （ 120 ± 12 ） ng/L, （ 124 ± 22） ng/L, （ P 〈 0.05 ） ]. Compared with A group, re-epithelization of the wound differentiation in all layers of epidermis were delayed in B group, with more severe wound swelling, exudation, and topical inflammatory response. The wound healing time in B group was （ 16.3 ± 1.6） d , which was obviously longer than that in A group[ （ 14.1 ± 1 . 8） d , P 〈 0.05 ） ]. Conclusion Sea water immersion combined with scald injury can aggravate the inflammatory response of the wound and delay the wound healing process.
出处
《中华烧伤杂志》
CAS
CSCD
北大核心
2006年第3期215-217,共3页
Chinese Journal of Burns
基金
广东省医学科学技术研究课题资助项目(A2004386)
