摘要
目的检测IGF信号通路关键蛋白IGF 1,IGF 1R和AKT在原发性肺腺癌中的表达,探讨其与临床病理学特征和生存时间的关系。方法采用免疫组化方法和免疫印迹技术检测IGF 1,IGF 1R和AKT在31例原发性肺腺癌及12例良性肺病变组织中的表达。结果IGF 1、IGF 1R和AKT在肺腺癌中的表达率分别为41.9%(13/31)、67.7%(21/31)和51.6%(16/31),显著高于良性肺组织(P值分别为0.0252、0.0016和0.0071)。IGF 1和IGF 1R及IGF 1和AKT在肺腺癌中表达呈显著相关(P值分别为0.0344和0.0179)。晚期肺癌(Ⅲ+Ⅳ)IGF 1和IGF 1R表达显著高于早期(Ⅰ+Ⅱ)(P值分别为0.0109和0.0303)。IGF 1、IGF 1R和AKT在伴有淋巴结转移肺癌中的表达显著高于无淋巴结转移肺癌(P值分别为0.0468、0.0490和0.0443)。低分化肺癌中IGF 1和IGF 1R表达显著高于中或高分化肺癌(P值分别为0.0484和0.0291)。IGF 1和IGF 1R阳性患者的生存时间显著短于阴性者(IGF 1:10比14个月,P=0.0103;IGF 1R:13比26个月,P=0.0056)。IGF 1和IGF 1R是肺腺癌预后的影响因素,AKT无预后意义。结论IGF信号通路关键蛋白IGF 1、IGF 1R和AKT表达在肺腺癌的发生和发展中可能起重要作用,进一步的研究有望展示其在肺癌预后和治疗方面的意义。
Objective To investigate the expression of IGF1, IGF1R and AKT, the componets of IGF signaling pathway, in primary adenocarcinoma of the lung as well as the correlation between expression and clinicopathological characteristics and clinical follow-up. Methods The expression of IGFI,IGF1R and AKT was investigated in 31 specimens from patients with primary adenocarcinoma of the lung and in 12 specimens from patients with benign lung diseases by immunohistochemistry and Western blotting. Results IGF1,IGF1R and AKT were overexpressed in cancer compared with benign lung tissues （P= 0. 0252,0. 0016 and 0. 0071 ,respectively）. IGF1 and IGF1R as well as IGF1 and AKT expressions were strongly associated with each other in adenocarcinoma （P= 0. 0344 and 0. 0179,respectively）. The expression of IGF1 and IGF1R was significantly higher in lung cancers with higher stages （ Ⅰ ＋ Ⅳ） than in those with lower stages （ Ⅰ ＋Ⅱ ） （P =0. 0109 and 0. 0303,respectively）. IGF1 ,IGF1R and AKT were statistically higher in lung cancers with metastasis in local lymph node than in those without metastasis in local lymph node （P=0. 0468,0. 0490 and 0. 0443 , respectively）. IGF1 and IGF1R were significantly higher in tumors with poor differentiation than in those with moderate or well differentiation （P= 0. 0484 and 0. 0291,respectively）. IGF1 or IGF1R positive cases were correlated with significantly shorter patient survival （median, 10 months for IGF1 positive cases versus 14 months for negative cases,P=0. 0103; 13 months for IGF1R positive cases versus 26 months for negative cases,P= 0. 0056）. Univariate but not multivariate analysis revealed that IGF1 and IGF1R expressions were the prognostic factors of adenocarcinoma of lung. However,AKT was of no significance. Conclusions The expression of IGF1,IGF1R and AKT,the components of IGF signaling pathway, may play an important role in the initiation and progression of human adenocarcinoma of the lung. Further investigation in this pathway may reveal its
出处
《实用肿瘤杂志》
CAS
2006年第1期15-19,共5页
Journal of Practical Oncology
基金
教育部留学回国人员科研启动基金资助项目[(2005)55]
湖北省自然科学基金项目(2004ABA194)
