摘要
采用乙型脑炎重组痘苗病毒Ｊ１和Ｊ３株，两者均含乙脑病毒（ＪＥＶ）的Ｅ、ＮＳ１和ＮＳ２Ａ基因，后者还含ＰｒＭ基因，在ＢＨＫ－２１－１５细胞中培养表达，经免疫荧光法（ＩＦＡ）测定，两者均可高效表达ＪＥＶ特异性抗原。用该重组病毒免疫瑞士种小鼠，经ＩＦＡ检测证明Ｊ１、Ｊ３均可诱生抗ＪＥＶ抗体，其中Ｊ３免疫鼠还产生中和抗体（Ｎ）和血抑抗体（ＨＩ），且其抗ＪＥＶ攻击能力显著增高。
he Third Military Medical University)Two JEV-vaccinia recombinants J1 and J3 were engineered for expression of the JEVantigens. Both J1 and J3 contained the E,NS1 and NS2A genes,J3 had the PrM gene inaddition to the above three genes. Indirect immunofluorescent assay revealed that J1 and J3expressed JEV antigens in recombinant-infected BHK cells. Immunization of 15-day-oldmice with the recombinants resulted in immune response to JEV. Mice vaccinated with J3produced both hemagglutinin-inhibiting and virus-neutralizing antibodies to JEV and showedincreased resistance to JEV chanllenge;while J1 had no such effects.Therefore PrM gene of J3played an important role in inducing protective immune response.
出处
《病毒学报》
CAS
CSCD
北大核心
1995年第4期317-321,共5页
Chinese Journal of Virology
关键词
乙型脑炎
重组痘苗病毒
抗原表达
免疫保护性
Japanese encephalitis virus,JEV recombinant vaccinia virus,Antigen expression,Immune protection
