摘要
采用线栓法制成大鼠大脑中动脉缺血再灌注模型,用地高辛精标记C-fos探针进行原位杂交。结果示缺血再灌注鼠栓塞侧和丹参组栓塞侧皮层及海马C-fos基因表达显著增多,但丹参组栓塞侧C-fos基因表达显著低于缺血再灌组(P<0.05),而两组栓塞对侧比较无显著差异。表明,脑缺血再灌注后脑组织C-fos基因表达显著增多,丹参能部分抑制缺血后C-fos基因的表达。
The local cerebral ischemia and reperfusion model of rats was made with thread embolism of middle cerebral artery. After 90 min ischemia following 30 min reperfusion, C-fos mRNA expression in rat brain was investigated with in situ hybridization in ischemia following reperfusion and RSM-treated group. The results showed that C-fos gene expression in cerebral cortex and hippocampus of ischemic sides was significantly increased as compared with nonischemic sides (P<0. 001). Although C-fos gene expression in RSM treated-rat was also increased, yet it was significantly lower than that of ischemia following reperfusion group (P<0. 05). The results indicated that C-fos gene expression in rat brain can be induced by cerebral ischemia following reperfusion and RSM may partly play a role in inhibition of C-fos gene expression during ischemia and reperfusion.
出处
《临床神经科学》
1995年第2期63-65,共3页
Chinese Journal of Clinical Neurosciences
关键词
脑缺血
再灌注
丹参
C-FOS基因
Ischemia Reperfusion Radix Salviae Miltiorrhizae (RSM) C-fos gene
