摘要
目的 :观察N端缺失 2 7个氨基酸残基的人酸性成纤维细胞生长因子突变体 (mhaFGF)对肝癌细胞增殖活性和信号转导的影响。方法 :用不同浓度 (0 0 1- 10 0 μg/L)的人酸性成纤维细胞生长因子 (haFGF)及mhaFGF分别处理肝癌细胞 ,15min后用半定量Westernblotting检测信号蛋白Grb2、Erk1/2在肝癌细胞中的表达 ,4 8h后用MTT法检测细胞增殖活性 ,并用流式细胞仪分析细胞周期。结果 :haFGF组G1期细胞比例显著低于和S期显著高于mhaFGF组和对照组 ,而mhaFGF组与对照组间无差别 ;mhaFGF组的Grb2和MAPK -ERk1/2信号分子表达水平低于haFGF组。结论 :mhaFGF促分裂活性的下降可能是通过下调MAPK信号通路中的MAPK -ERK1/2和Grb2信号分子的表达来实现的。
AIM: To observe the effects of the human acidic fibroblast growth factor mutant (mhaFGF), lacking 27 amino acids at N-terminal, on the proliferation and signal transduction of the hepatocarcinoma cells. METHODS: The hepatocarcinoma cells were treated with human acidic fibroblast growth factor (haFGF) and mhaFGF, respectively. The expression levels of the signal proteins, Grb2 and Erk1/2, in the hepatocarcinoma cells were detected by semi-quantitative Western-blotting after treated for 15 min. The mitogenic activity of both haFGF and mhaFGF was detected by MTT method and the cell cycle was analysed by flow cytometer (FCM) after treated for 48 h. RESULTS: The mitogenic activity and the ratio of G 1 and S phase cells in mhaFGF-treated cells were markedly lower than that of the haFGF, and close to that of the control group. The expression level of both Grb2 and Erk1/2 in the mhaFGF-treated cells were lower than those in the haFGF- treated cells. CONCLUSION: The decrease in the mitogenic activity of mhaFGF is probably associated to its down-regulating the expression of the signal molecular, MAPK-ERK1/2 and Grb2.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2005年第3期446-448,共3页
Chinese Journal of Pathophysiology
基金
国家 8 6 3计划资助项目 (No .2 0 0 1AA2 15 131
No.2 0 0 2AA2Z3318)
广东省自然科学基金资助项目 (No .0 10 4 2 4 )
关键词
成纤维细胞生长因子1
信号转导
肝肿瘤
Fibroblast growth factor 1
Signal transduction
Liver neoplasms
