摘要
本文将过氧化氢酶与经活化处理的醋酸纤维膜共价固定,并与氧电极偶合,研制成静态和流通二种过氧化氢生物电极.研究了醋酸纤维膜的活化、酶的固定化、测试介质等有关的实验条件和参数、测得静态和流通二种电极响应的线性范围分别为6.7×10^(-5)～2.0×10^(-3)mol/L和1.3×10^(-1)～1.0×10^(-3)mol/L.将电极用于实际试样中的回收率测定时,获得了较好的结果.
The catalase has been immobilized on acetyl cellulose membrane activated by pre-treatment with sodium periodate. The enzyme immobilized by covalent bounding coupled with an oxygen probe has been utilized in constructing the static or (low-through hydrogen peroxide sensor. The experimental condition for the activation of acetyl cellulose membrane and immobilization of the enzyme, as well as the measurement medium has been investigated. The linear response range for the static and flow-through versions of the sensor was 6. 7 X10-5~2.0X10-3 and 1. 3X 10-4~1.0X10-3mol/L, respectively. Satisfatory recoveries were obtained in the application of the proposed sensors for real sample analysis.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1994年第10期998-1001,共4页
Chinese Journal of Analytical Chemistry
基金
国家教委博士基金资助项目
关键词
醋酸纤维膜
过氧化氢
酶电极
Acetyl cellulose membrane,Catalase,Enzyme electrode,Biosensor.
