摘要
研究血管紧张素Ⅱ - 1型受体拮抗剂缬沙坦对二甲基亚硝胺 (DMN)大鼠肝纤维化模型MMP2mRNA的表达。制备DMN诱导的大鼠肝纤维化模型 ,同时缬沙坦灌胃 2 0mg·kg-1·d-1,共 8周 ,大鼠肝组织明胶酶A(MMP2 )mRNA的水平以逆转录多聚酶链反应 (RT -PCR)方法检测。缬沙坦可明显改善DMN肝纤维化的程度 ,模型组MMP2mRNA的水平高于正常对照组 ,缬沙坦预防性治疗组MMP2mRNA水平低于模型组 (P <0 0 1)。缬沙坦可抑制MMP2mRNA的表达 。
To observe MMP2 gene expression and the effect of angiotensin Ⅱ tape 1 receptor blocker valsartan on hepatic fibrosis induced by dimethylnitrosamine in rats.Except rats in control group,all were given intraperitoneal injections of 1% dimethylnitrosamine (DMN lml/kg、three or two consecutive days/a week for 6 weeks),From the first day of the intraperitoneal injection,rats in treatment groups were given valsartan for 8 weeks by gastric lavage.Liver tissue and blood samples of all rats were examined on 56 days(8 weeks).Hepatic mRNA levels of MMP2 evaluated by reverse-transcription polymerase chain reaction(RT-PCR).Valsartan significantly attenuated the degree of liver fibrosis.Gene expression of MMP2 mRNA was upregulated in DMN treated rats compared with normal rats.Valsartan downregulated the elevation of the mRNA levels(P<0.01).Valsartan can retard the progression of hepatic fibrosis and downregulated the MMP2 mRNA level.
出处
《临床肝胆病杂志》
CAS
2005年第1期19-20,共2页
Journal of Clinical Hepatology
基金
北京中医药科技发展基金项目资助 (编号 2 0 0 1 2 6)
