期刊文献+

芦苇抑藻化感物质的分离及其抑制蛋白核小球藻效果研究 被引量:79

Isolation and Effects on Green Alga Chlorella pyrenoidosa of Algal-Inhibiting Allelochemicals in the Macrophyte, Phragmites communis Tris.
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摘要 采用乙醇提取、溶剂萃取法从芦苇中分离出 2组能抑制蛋白核小球藻生长的化感物质 .该 2组化感物质对蛋白核小球藻的半效应浓度 (EC50 )分别为 0 6 8mg/L和 2 6 5mg/L .将前者用碱性氧化铝层析柱分离得到一组抑藻活性更强的化感物质和一组能够促进蛋白核小球藻生长的化感物质 :前者对蛋白核小球藻的EC50 为 0 5 0mg/L ,是目前报道的抑藻能力最强的化感物质 ;后者在浓度为 0 6 3mg/L时能显著促进蛋白核小球藻的生长 . Two fractions of secondary metabolites which inhibited the growth of green alga Chlorella pyrenoidosa, was isolated by several separation techniques from the ethanol extract of the macrophyte, Phragmites communis Tris. The EC 50 of the two fractions on C. pyrenoidosa were 0 68mg/L(the fraction of acidic solvent) and 2 65mg/L(the fraction of alkaline solvent), respectively. Further purification of the allelochemicals in the acidic solvent by alkaline aluminum oxide column chromatography revealed that this fraction containing inhibiting allelochemicals and stimulating allelochemicals The inhibiting allelochemicals showed more inhibitory effect and the EC 50 was 0 50mg/L. The result suggests that allelochemicals in P. communis can be used as an algae inhibitor.
出处 《环境科学》 EI CAS CSCD 北大核心 2004年第5期89-92,共4页 Environmental Science
基金 国家重点基础研究发展规划 ( 973 )项目 (G1990 45 711)
关键词 芦苇 蛋白核小球藻 抑制 化感作用 Chlorella pyrenoidosa Phragmites communis Tris. inhibition allelopathy
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参考文献16

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