摘要
目的 探讨脂肪对猪皮肤损伤的促愈作用 ,为临床皮肤损伤修复提供新的治疗方法。方法 6只小型香猪背部中线两侧各制备 4个 2 5cm×2 5cm的全层皮肤缺损创面 ,将创面随机分为 4组 ,即空白对照组、脂肪治疗组、碱性成纤维生长因子 (bFGF)治疗组和表皮生长因子(EGF)治疗组 (每组各 12个创面 )。分别于伤后 3、7、14、2 1天采用大体观察和组织学方法动态观察创面愈合情况。结果 伤后 3天和 7天 ,脂肪治疗组肉芽组织形成丰富 ,血管密度大 ,伤腔容积小于其余各组 (P <0 0 1) ;伤后 7天和 14天 ,各组创面逐渐缩小 ,脂肪治疗组创面面积明显小于其余各组(P <0 0 1) ,并且再上皮化的新生表皮较其余各组为厚。结论 自体脂肪移植到局部皮肤创面后能明显促进创面愈合速度 。
Objective To explore the effects of fat on healing of porcine skin wounds so as to provide a new strategy to promote skin wound repair after injury. Methods Forty-eight full-thickness skin wounds were produced on both sides of the back in 6 male minipigs (8 wounds in each animal). These wounds were then randomly divided into 4 groups, i.e. saline control group, fat autografting group, fibroblast growth factor (bFGF) treatment group and epidermal growth factor (EGF) treatment group. On day 3, 7, 14 and 21 after wounding, the area and the volume of wounds were measured, and histological examination was performed to evaluate the speed and quality of wound healing in different groups. Results On days 3 and 7, the amount of granulation tissue and vessel density in fat treatment group were significantly more abundant compared with other groups. Wound area and volume in fat treatment wounds were markedly decreased compared with those in other groups (P<0.01). Regenerated epidermis in fat treatment group was thicker than that of other groups. Conclusion These results confirmed that the speed of wound healing and quality of healing in wounds treated with fat autografting were enhanced, indicating that fat had a potential to accelerate the speed of healing and improve the quality of healing of open wound of skin.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2004年第7期577-579,共3页
Medical Journal of Chinese People's Liberation Army
基金
国家重点基础研究发展规划 (编号G1 9990 542 0 4 )
国家自然科学基金(编号 30 1 70 966)
国家自然科学基金重点项目 (编号 30 2 30 370 )
国家杰出青年科学基金 (编号 3952 50 2 4 )资助课题
