摘要
以大豆耐冷品种Kottman和铁丰31号、冷敏感品种沈农8号和东农46号为材料,以20℃为对照研究在10℃和6℃低温下萌发过程中大豆抗氧化保护酶超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)的活性变化.结果表明,随温度降低,抗冷性不同的大豆品种抗氧化酶活性变化表现出明显的差异.在6℃下,在萌发的中后期,耐冷品种铁丰31号和Kottman的SOD活性仍相对稳定并维持较高活性,POD活性迅速上升,并一直显著高于冷敏感品种.在6℃下,耐冷品种的CAT活性在萌发初期(0～9 d)有大幅下降,然后随着萌发的进程而缓慢上升,而冷敏感品种CAT活性在萌发的(0～9 d)大幅下降,此后小幅下降后又缓慢回升.试验表明,耐冷大豆品种在萌发期间的SOD、POD、CAT活性对低温反应灵敏,低温处理后活性较高或较稳定;耐冷品种和冷敏感品种的CAT活性变化在低温(6℃)下的差异最大,这表明过氧化氢酶在大豆种子萌发过程的活性氧代谢中可能具有较关键的作用.
The chilling-tolerant soybean cultivars Kottman and Tiefeng 31 and the chilling-sensitive soybean Shennong 8 and Dongnong 46 were used as experimental materials.Their seeds were germinated under low temperature,10 ℃ and 6 ℃.The changes of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT) activities were investigated,compared with those under 20℃,during seed germination stage.The results showed that the soybean cultivars with different cold tolerance presented remarkably change trends in the defense enzyme activities as the temperature decrease.Under 6 ℃,the tolerant cultivars Kottman and Tiefeng 31 presented a relatively stable and higher SOD activity during the middle and late stage of germination,and POD activity increased rapidly as the germination develops and was notably higher than the sensitive ones in all the process.Under the same low temperature,CAT activity in the tolerant cultivars and the sensitive cultivars decreased dramatically during the early stage of germination(0~9 d),then the tolerant cultivars CAT activity increased slightly as the germination develops,but the sensitive cultivars,CAT activity decreased slowly during the stage of germination(9~15 d),then increased slowly.These results suggested that SOD,POD and CAT activities in the tolerant soybean cultivars response quickly to low temperature during seed germination period,which activities were higher or more stable compared with the sensitive cultivars.There was a significant difference in CAT activity between the tolerant and sensitive cultivars under 6 ℃.This implied that CAT may play a pivotal role in reactive oxygen species metabolism during soybean germination.
出处
《大豆科学》
CAS
CSCD
北大核心
2007年第2期171-175,共5页
Soybean Science
基金
辽宁省重点攻关项目(2001406002)
关键词
低温
种子萌发
保护酶
Soybean
Low temperature
Germination
Defense enzyme
