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水稻核心种质表型性状遗传多样性分析及综合评价 被引量:153
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作者 胡标林 万勇 +4 位作者 李霞 雷建国 罗向东 严文贵 谢建坤 《作物学报》 CAS CSCD 北大核心 2012年第5期829-839,共11页
种质资源是水稻遗传育种和解析复杂性状的重要基础,而对遗传多样评估有利于鉴定最优亲本组合,以产生遗传变异最大的后代群体和促进不同资源的有利基因渗透到栽培品种。选用14个表型性状对美国农业部水稻核心种质中的六大洲1579份水稻种... 种质资源是水稻遗传育种和解析复杂性状的重要基础,而对遗传多样评估有利于鉴定最优亲本组合,以产生遗传变异最大的后代群体和促进不同资源的有利基因渗透到栽培品种。选用14个表型性状对美国农业部水稻核心种质中的六大洲1579份水稻种质,分析与评价其遗传多样性和优良稻种资源,主要结果如下:(1)亚洲、非洲与大洋洲种质间遗传距离较远,且亚洲、非洲和大洋洲的水稻资源具有较丰富的表型遗传多样性,而不同性状的遗传多样性在洲际间表现不同;粒长宽比、碱消值、株高、粒宽、千粒重和淀粉含量6个性状具有很高的表型遗传多样性。(2)采用主成分分析法和逐步回归分析法综合评判表明,越南的PI392768的综合性状表现最好,法国的PI281760综合性状表现最差,同时淀粉含量、抽穗期、株高、倒伏性、糙米色和颖壳色6个性状可作为种质资源综合评价指标。在水稻育种中应注重利用具有丰富遗传多样性的种质资源,并在亲本选配时适当选择遗传距离较远且综合性状表现差异大的种质材料。 展开更多
关键词 核心种质 表型性状 遗传多样性 主成分分析 综合评价
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水稻抗稻瘟病基因Pi-ta的分子标记辅助选择 被引量:108
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作者 王忠华 贾育林 +1 位作者 吴殿星 夏英武 《作物学报》 CAS CSCD 北大核心 2004年第12期1259-1265,共7页
利用已建立的水稻抗稻瘟病基因Pi ta显性分子标记对 30个品系和 1 57个来自不同国家的一些水稻品种进行分子鉴定 ,并采用稻瘟病菌菌株ZN57(IC 1 7)和ZN6 1 (IB 4 9)人工接种试验进行致病性测试。结果表明 ,大部分品系和少数水稻品种含... 利用已建立的水稻抗稻瘟病基因Pi ta显性分子标记对 30个品系和 1 57个来自不同国家的一些水稻品种进行分子鉴定 ,并采用稻瘟病菌菌株ZN57(IC 1 7)和ZN6 1 (IB 4 9)人工接种试验进行致病性测试。结果表明 ,大部分品系和少数水稻品种含抗病基因Pi ta ,且对稻瘟病菌菌株ZN57和ZN6 1表现抗病反应。除此之外 ,利用两对显性分子标记YL1 55 YL87和YL1 83 YL87对 350个杂交F3代株系进行早期筛选 ,得到 1 1 8个抗病基因Pi ta纯合的株系。这些株系田间抗性调查结果表明 ,抗病基因存在与否与田间抗性相吻合。因Pi ta基因与许多其他抗病基因紧密连锁 ,而使含有Pi ta基因的品种具有广谱抗性 ,由此确立了Pi ta基因显性分子标记在育种辅助选择中的应用价值。 展开更多
关键词 水稻 抗稻瘟病基因Pi-ta 分子标记 辅助选择
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Estimate of CH_4 Emissions from Year-Round Flooded Rice Fields During Rice Growing Season in China 被引量:97
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作者 CAIZu-Cong KANGGuo-Ding +1 位作者 H.TSURUTA A.MOSIER 《Pedosphere》 SCIE CAS CSCD 2005年第1期66-71,共6页
A special kind of rice field exists in China that is flooded year-round. These rice fields have substantially large CH4 emissions during the rice-growing season and emit CH4 continuously in the non-rice growing season... A special kind of rice field exists in China that is flooded year-round. These rice fields have substantially large CH4 emissions during the rice-growing season and emit CH4 continuously in the non-rice growing season. CH4 emission factors were used to estimate the CH4 emissions from year-round flooded rice fields during the rice-growing season in China. The CH4 emissions for the year-round flooded rice fields in China for the rice growing season over a total area of 2.66 Mha were estimated to be 2.44 Tg CH… 展开更多
关键词 IPCC methodology methane emission rice fields year-round flooded
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基于表型性状的中国大蒜资源遗传多样性分析 被引量:76
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作者 王海平 李锡香 +4 位作者 沈镝 邱杨 宋江萍 张晓辉 Philipp W.Simon 《植物遗传资源学报》 CAS CSCD 北大核心 2014年第1期24-31,共8页
对资源圃保存的212份大蒜种质资源的表型性状进行了系统鉴定,分析表明我国大蒜种质资源的表型变异丰富。主成分分析表明,29个性状可以归并为反映植株生长发育、产品特征和产量构成的8个主成分;进一步的聚类分析将所有资源分为特点明显的... 对资源圃保存的212份大蒜种质资源的表型性状进行了系统鉴定,分析表明我国大蒜种质资源的表型变异丰富。主成分分析表明,29个性状可以归并为反映植株生长发育、产品特征和产量构成的8个主成分;进一步的聚类分析将所有资源分为特点明显的2类5亚类。为了避免质量性状在种质评价中的主导作用,与产量相关的鳞茎数量性状的主成分分析显示,前3个主成分累积贡献率达74.83%,第一主成分中鳞茎重、鳞茎直径、鳞茎高和鳞芽数是影响产量的主要因子。主坐标排序将所有资源分为6类。通过综合评价,将大蒜鳞茎产量分为6个级别,筛选出单产大于15 t/hm2的资源3份。 展开更多
关键词 大蒜 遗传多样性 表型性状
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抗菌肽MB_(39)基因导入‘皇家嘎啦’苹果及其四倍体植株的培育 被引量:31
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作者 刘庆忠 赵红军 +2 位作者 刘鹏 Mong Rengong FreddiA.Hammerschlag 《园艺学报》 CAS CSCD 北大核心 2001年第5期392-398,共7页
采用农杆菌介导法 ,将抗菌肽MB39基因转入‘皇家嘎啦’苹果 ,获得了 7个转基因株系。通过秋水仙素诱变染色体组工程育种技术 ,由转化体叶片获得了 2 0个四倍体植株。经PCR检测和SouthernBlotting杂交证明 ,抗菌肽MB39基因已经整合到皇... 采用农杆菌介导法 ,将抗菌肽MB39基因转入‘皇家嘎啦’苹果 ,获得了 7个转基因株系。通过秋水仙素诱变染色体组工程育种技术 ,由转化体叶片获得了 2 0个四倍体植株。经PCR检测和SouthernBlotting杂交证明 ,抗菌肽MB39基因已经整合到皇家嘎啦苹果的染色体组中。转基因株系TR 1、TR 3提高了对火疫病的抗性。采用流式细胞技术 (FlowCytometry) 展开更多
关键词 抗菌肽MB39 转基因 四倍体 秋水仙素 皇家嘎啦苹果 农杆菌介导
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植物抗病分子机制研究进展 被引量:30
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作者 王忠华 贾育林 夏英武 《植物学通报》 CSCD 北大核心 2004年第5期521-530,共10页
近十年来,植物抗病分子机制研究取得显著进展。综述了植物抗病基因的克隆及其结构分析、病原菌无毒基因及其相关致病因子的克隆与研究、信号传导相关因子的克隆及其结构分析以及植物-病原菌的相互作用研究,重点介绍了以植物特异抗病基... 近十年来,植物抗病分子机制研究取得显著进展。综述了植物抗病基因的克隆及其结构分析、病原菌无毒基因及其相关致病因子的克隆与研究、信号传导相关因子的克隆及其结构分析以及植物-病原菌的相互作用研究,重点介绍了以植物特异抗病基因为介导的诱导防卫作用机制(包括抗病基因编码毒素蛋白,进而抑制病原菌的繁殖;显性基因编码病原菌致病性的靶标物;抗病基因表达产物直接引发抗病反应和基因对基因的抗病作用机制等)的研究进展,以期为植物抗病育种提供有益的信息。 展开更多
关键词 植物 抗病基因 信号分子 育种 无毒基因
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OsWRKY62 is a Negative Regulator of Basal and Xa21-Mediated Defense against Xanthomonas orvzae pv. orvzae in Rice 被引量:47
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作者 Ying Peng Laura E. Bartley +5 位作者 Xuewei Chen Christopher Dardick Mawsheng Chern Randy Ruan Patrick E. Canlas Pamela C. Ronald 《Molecular Plant》 SCIE CAS CSCD 北大核心 2008年第3期446-458,共13页
The rice Xa21 gene, which confers resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo), encodes a receptor-like kinase, Few components involved in transducing the Xa21-mediated defense response h... The rice Xa21 gene, which confers resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo), encodes a receptor-like kinase, Few components involved in transducing the Xa21-mediated defense response have yet been identified. Here, we report that XA21 binds to a WRKY transcription factor, called OsWRKY62. The OsWRKY62 gene encodes two splice variants (OsWRKY62.1 and OsWRKY62.2). OsWRKY62.1:smGFP2 and OsWRKY62.2:smGFP2 fusion pro- teins partially localize to the nucleus. Transgenic plants overexpressing OsWRKY62.1 are compromised in basal defense and Xa21-mediated resistance to Xoo. Furthermore, overexpression of OsWRKY62.1 suppresses the activation of defenserelated genes. These results imply that OsWRKY62 functions as a negative regulator of innate immunity in rice, and serves as a critical mediator of both basal and race-specific defense responses. 展开更多
关键词 RICE OsWRKY62 XA21 defense response.
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Drift and deposition of ultra-low altitude and low volume application in paddy field 被引量:46
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作者 Xue Xinyu Tu Kang +2 位作者 Qin Weicai Yubin Lan Huihui Zhang 《International Journal of Agricultural and Biological Engineering》 SCIE EI CAS 2014年第4期23-28,共6页
Field trials were performed to evaluate various techniques for measuring spray deposition and aerial drift during spray application to paddy field.The application of a spraying agent containing the fluorescent dye Rho... Field trials were performed to evaluate various techniques for measuring spray deposition and aerial drift during spray application to paddy field.The application of a spraying agent containing the fluorescent dye Rhodamine-B was applied by an unmanned aerial vehicle(UAV)which flew at a height of 5 m,a speed of 3 m/s,and the wind speed of 3 m/s.The results showed that because the downdraft produced by a helicopter rotor increased the penetrability of crops,there is a higher deposition on the upper layer and the under layer than the traditional spraying.The average deposition on the upper layer accounts for 28% of the total spraying,the deposition on the under layer accounts for 26% of the total spraying.The deposition on the under layer takes up 92.8% of the deposition on the upper layer.Droplets drift data showed that the drift of non-target area took up 12.9% of the total liquid spray.The 90% drifting droplets were located within a range of 8 m of the target area;the drift quantity was almost zero at a distance of 50 m away from the treated area. 展开更多
关键词 paddy field ultra-low altitude low volume UAV droplet drift DEPOSITION
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Carotenoid metabolism and regulation in horticultural crops 被引量:45
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作者 Hui Yuan Junxiang Zhang +1 位作者 Divyashree Nageswaran Li Li 《Horticulture Research》 SCIE 2015年第1期123-133,共11页
Carotenoids are a diverse group of pigments widely distributed in nature.The vivid yellow,orange,and red colors of many horticultural crops are attributed to the overaccumulation of carotenoids,which contribute to a c... Carotenoids are a diverse group of pigments widely distributed in nature.The vivid yellow,orange,and red colors of many horticultural crops are attributed to the overaccumulation of carotenoids,which contribute to a critical agronomic trait for flowers and an important quality trait for fruits and vegetables.Not only do carotenoids give horticultural crops their visual appeal,they also enhance nutritional value and health benefits for humans.As a result,carotenoid research in horticultural crops has grown exponentially over the last decade.These investigations have advanced our fundamental understanding of carotenoid metabolism and regulation in plants.In this review,we provide an overview of carotenoid biosynthesis,degradation,and accumulation in horticultural crops and highlight recent achievements in our understanding of carotenoid metabolic regulation in vegetables,fruits,and flowers. 展开更多
关键词 CROPS FLOWERS METABOLISM
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Carotenoid Metabolism in Plants: The Role ol Plastids 被引量:43
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作者 Tianhu Sun Hui Yuan +3 位作者 Hongbo Cao Mohammad Yazdani Yaakov Tadmor Li Li 《Molecular Plant》 SCIE CAS CSCD 2018年第1期58-74,共17页
Carotenoids are indispensable to plants and critical in human diets. Plastids are the organelles for carotenoid biosynthesis and storage in plant cells. They exist in various types, which include proplastids, etioplas... Carotenoids are indispensable to plants and critical in human diets. Plastids are the organelles for carotenoid biosynthesis and storage in plant cells. They exist in various types, which include proplastids, etioplasts, chloroplasts, amyloplasts, and chromoplasts. These plastids have dramatic differences in their capacity to synthesize and sequester carotenoids. Clearly, plastids play a central role in governing carotenogenic activity, carotenoid stability, and pigment diversity. Understanding of carotenoid metabolism and accumulation in various plastids expands our view on the multifaceted regulation of carotenogenesis and facilitates our efforts toward developing nutrient-enriched food crops. In this review, we provide a comprehensive overview of the impact of various types of plastids on carotenoid biosynthesis and accumulation, and discuss recent advances in our understanding of the regulatory control of carotenogenesis and metabolic engineering of carotenoids in light of plastid types in plants. 展开更多
关键词 CAROTENOID PLASTID regulation METABOLISM metabolic engineering
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Spatial-Temporal Pattern and Driving Forces of Land Use Changes in Xiamen 被引量:41
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作者 QUAN Bin CHEN Jian-Fei +4 位作者 QIU Hong-Lie M. J. M. ROMKENS YANG Xiao-Qi JIANG Shi-Feng LI Bi-Cheng 《Pedosphere》 SCIE CAS CSCD 2006年第4期477-488,共12页
Using Landsat TM data of 1988, 1998 and 2001, the dynamic process of the spatial-temporal characteristics of land use changes during 13 years from 1988 to 2001 in the special economic zone of Xiamen, China was analyze... Using Landsat TM data of 1988, 1998 and 2001, the dynamic process of the spatial-temporal characteristics of land use changes during 13 years from 1988 to 2001 in the special economic zone of Xiamen, China was analyzed to improve understanding and to find the driving forces of land use change so that sustainable land utilization could be practiced. During the 13 years cropland decreased remarkably by nearly 11304.95 ha. The areas of rural-urban construction and water body increased by 10 152.24 ha and 848.94 ha, respectively. From 1988 to 2001, 52.5% of the lost cropland was converted into rural-urban industrial land. Rapid urbanization contributed to a great change in the rate of cropland land use during these years. Land-reclamation also contributed to a decrease in water body area as well as marine ecological and environmental destruction. In the study area 1) urbanization and industrialization, 2) infrastructure and agricultural intensification, 3) increased affluence of the farming community, and 4) policy factors have driven the land use changes. Possible sustainable land use measures included construction of a land management system, land planning, development of potential land resources, new technology applications, and marine ecological and environmental protection. 展开更多
关键词 driving force GIS land use change remote sensing XIAMEN
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Inducible direct plant defense against insect herbivores: A review 被引量:39
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作者 Ming-Shun Chen 《Insect Science》 SCIE CAS CSCD 2008年第2期101-114,共14页
Plants respond to insect herbivory with responses broadly known as direct defenses, indirect defenses, and tolerance. Direct defenses include all plant traits that affect susceptibility of host plants by themselves. O... Plants respond to insect herbivory with responses broadly known as direct defenses, indirect defenses, and tolerance. Direct defenses include all plant traits that affect susceptibility of host plants by themselves. Overall categories of direct plant defenses against insect herbivores include limiting food supply, reducing nutrient value, reducing preference, disrupting physical structures, and inhibiting chemical pathways of the attacking insect. Major known defense chemicals include plant secondary metabolites, protein inhibitors of insect digestive enzymes, proteases, lectins, amino acid deaminases and oxidases. Multiple factors with additive or even synergistic impact are usually involved in defense against a specific insect species, and factors of major importance to one insect species may only be of secondary importance or not effective at all against another insect species. Extensive qualitative and quantitative high throughput analyses of temporal and spatial variations in gene expression, protein level and activity, and metabolite concentration will accelerate not only the understanding of the overall mechanisms of direct defense, but also accelerate the identification of specific targets for enhancement of plant resistance for agriculture. 展开更多
关键词 chemical defense defensive protease LECTINS protease inhibitor secondary metabolite
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Morphological and Physiological Responses of Plants to Cadmium Toxicity: A Review 被引量:38
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作者 HE Shanying YANG Xiaoe +1 位作者 Zhenli HE Virupax C.BALIGAR 《Pedosphere》 SCIE CAS CSCD 2017年第3期421-438,共18页
Cadmium(Cd) contamination has posed an increasing challenge to environmental quality and food security. In recent years,phytoremediation has been particularly scrutinized because it is cost-effective and environmental... Cadmium(Cd) contamination has posed an increasing challenge to environmental quality and food security. In recent years,phytoremediation has been particularly scrutinized because it is cost-effective and environmentally friendly, especially the use of metal-hyperaccumulating plants to extract or mine heavy metals from polluted soils. Under Cd stress, responses of hyperaccumulator and non-hyperaccumulator plants differ in morphological responses and physiological processes such as photosynthesis and respiration,uptake, transport, and assimilation of minerals and nitrogen, and water uptake and transport, which contribute to their ability to accumulate and detoxify Cd. This review aims to provide a brief overview of the recent progresses in the differential responses of hyperaccumulator and non-accumulator plants to Cd toxicity in terms of growth and physiological processes. Such information might be useful in developing phytoremediation technology for contaminated soils. 展开更多
关键词 heavy metal HYPERACCUMULATOR metal accumulation mineral elements PHOTOSYNTHESIS PHYTOREMEDIATION water trans-port water uptake
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Karyotype Stability and Unbiased Fractionation in the Paleo-Allotetraploid Cucurbita Genomes 被引量:37
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作者 Honghe Sun Shan WU +14 位作者 Guoyu Zhang Chen Jiao Shaogui Guo Yi Ren Jie Zhang Haiying Zhang Guoyi Gong Zhangcai Jia Fan Zhang Jiaxing Tian William J. Lucas Jeff J. Doyle Haizhen Li Zhangjun Fei Yong Xu 《Molecular Plant》 SCIE CAS CSCD 2017年第10期1293-1306,共14页
The Cucurbita genus contains several economically important species in the Cucurbitaceae family. Here, we report high-quality genome sequences of C. maxima and C. moschata and provide evidence supporting an allotetrap... The Cucurbita genus contains several economically important species in the Cucurbitaceae family. Here, we report high-quality genome sequences of C. maxima and C. moschata and provide evidence supporting an allotetraploidization event in Cucurbita. We are able to partition the genome into two homoeologous subgenomes based on different genetic distances to melon, cucumber, and watermelon in the Benincaseae tribe. We estimate that the two diploid progenitors successively diverged from Benincaseae around 31 and 26 million years ago (Mya), respectively, and the allotetraploidization happened at some point between 26 Mya and 3 Mya, the estimated date when C. maxima and C. moschata diverged. The subgenomes have largely maintained the chromosome structures of their diploid progenitors. Such long-term karyotype stability after polyploidization has not been commonly observed in plant polyploids. The two subgenomes have retained similar numbers of genes, and neither subgenome is globally dominant in gene expression. Allele-specific expression analysis in the C. maxima ×C. moschata interspecific F1 hybrid and their two parents indicates the predominance of trans-regulatory effects underlying expression divergence of the parents, and detects transgressive gene expression changes in the hybrid correlated with heterosis in important agronomic traits. Our study provides insights into polyploid genome evolution and valuable resources for genetic improvement of cucurbit crops. 展开更多
关键词 Cucurbita maxima Cucurbita moschata paleo-allotetraploid karyotype stability unbiased fractionation allele-specific expression
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The Plant Vascular System: Evolution, Development and Functions 被引量:36
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作者 William J. Lucas Andrew Groover +13 位作者 Raffael Lichtenberger Kaori Furuta Shri-Ram Yadav Yk Helariutta Xin-Qiang He Hiroo Fukuda Julie Kang Siobhan M. Brady John W. Patrick John Sperry Akiko Yoshida Ana-Flor López-Millan Michael A. Grusak Pradeep Kachroo 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2013年第4期294-388,共95页
The emergence of the tracheophyte-based vascular system of land plants had major impacts on the evolution of terrestrial biology, in general, through its role in facilitating the development of plants with increased s... The emergence of the tracheophyte-based vascular system of land plants had major impacts on the evolution of terrestrial biology, in general, through its role in facilitating the development of plants with increased stature, photosynthetic output, and ability to colonize a greatly expanded range of environmental habitats. Recently, considerable progress has been made in terms of our understanding of the developmental and physiological programs involved in the formation and function of the plant vascular system. In this review, we first examine the evolutionary events that gave rise to the tracheophytes, followed by analysis of the genetic and hormonal networks that cooperate to orchestrate vascular development in the gymnosperms and angiosperms. The two essentialfunctions performed by the vascular system, namely the delivery of resources (water, essential mineral nutrients, sugars and amino acids) to the various plant organs and provision of mechanical support are next discussed. Here, we focus on critical questions relating to structural and physiological properties controlling the delivery of material through the xylem and phloem. Recent discoveries into the role of the vascular system as an effective long-distance communication system are next assessed in terms of the coordination of developmental, physiological and defense-related processes, at the whole-plant level. A concerted effort has been made to integrate all these new findings into a comprehensive picture of the state-of-the-art in the area of plant vascular biology. Finally, areas important for future research are highlighted in terms of their likely contribution both to basic knowledge and applications to primary industry. 展开更多
关键词 EVOLUTION vascular development PHLOEM XYLEM nutrient delivery long-distance communication systemic signaling.
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稻谷短期贮藏过程中稻米品质变化 被引量:27
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作者 舒庆尧 吴殿星 +2 位作者 夏英武 高明尉 AnnaMcClung 《浙江农业学报》 CSCD 2000年第1期1-5,共5页
稻谷收获后在短期贮藏(4 个月) 过程中,稻米表观直链淀粉含量(AAC) 、糊化温度( 碱消值) 和碾磨品质均无明显变化,但香味主要成份2乙酰1吡洛淋(2AP) 含量明显下降,Della 在5 ℃和35℃下贮藏4 个... 稻谷收获后在短期贮藏(4 个月) 过程中,稻米表观直链淀粉含量(AAC) 、糊化温度( 碱消值) 和碾磨品质均无明显变化,但香味主要成份2乙酰1吡洛淋(2AP) 含量明显下降,Della 在5 ℃和35℃下贮藏4 个月后,2AP的含量分别下降了17.8% 和68 .1 % ;用快速粘度分析仪(Rapid ViscoAnalyser,RVA) 测得的稻米淀粉谱(RVA谱) 的崩解值和碱消值,以及米饭的硬度和粘性在贮藏过程中增加或下降,这种变化在低温(5 ℃) 下较小,在高温(35 ℃) 下较大。如35 ℃下贮藏4 个月后,Della和Skybonnet2 个水稻品种的米饭硬度分别增加了15.7% 和16 .4 % ,而粘度则分别下降了23-3 % 和31 .7 % 。 展开更多
关键词 贮藏 温度 籽粒品质 水稻 育种
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228份引进大蒜资源的表型多样性分析及适应性初步评价 被引量:33
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作者 都真真 李锡香 +7 位作者 宋江萍 武亚红 赵青 徐婷 张晓辉 Barbara Hellier Jinguo Hu 王海平 《植物遗传资源学报》 CAS CSCD 北大核心 2019年第5期1186-1196,共11页
为了解引进大蒜资源遗传多样性和引进后的适应性,对国家无性繁殖及多年生蔬菜种质资源圃从34个国家引进的228份大蒜(Allium sativum L.)资源进行了表型性状评价。结果表明:13个数量性状的变异系数分布在18.94%~56.36%之间,质量性状中除... 为了解引进大蒜资源遗传多样性和引进后的适应性,对国家无性繁殖及多年生蔬菜种质资源圃从34个国家引进的228份大蒜(Allium sativum L.)资源进行了表型性状评价。结果表明:13个数量性状的变异系数分布在18.94%~56.36%之间,质量性状中除叶形仅一种表现型,其他质量性状表型丰富;25个性状的多样性指数分布在0~2.03之间,遗传多样性指数高,类型丰富。通过聚类分析,将引进的大蒜资源分为3个类群,类群Ⅰ地上部分植株矮小、细弱,叶片短而细,鳞茎表现较差,为丰产性较差的类群;类群Ⅱ鳞茎横茎小,鳞芽数少,鳞茎形状为高圆型;类群Ⅲ植株高大开展,叶片长且宽,鳞茎重且鳞茎横茎大。主成分分析中,选取累积贡献率为69.94%的3个因子来评价该批资源。大蒜鳞茎部分性状的相关分析表明,鳞茎横径、鳞茎高、鳞芽高、鳞芽背宽均与单头鳞茎重存在极显著的正相关关系,在选育高产大蒜品种时,这些性状将作为主要的目标性状;引种前后的鳞茎相关性状对比分析表明,不同种质在引种后的适应性表现差异较大,部分资源种植后鳞茎高和鳞茎横茎较引种时明显增大,表现出了较好的适应性。相关结果将为大蒜种质资源的利用及品种选育等提供重要参考。 展开更多
关键词 大蒜 引种 种质资源 遗传多样性 适应性
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PCR和Dig—cRNA探针检测番茄环斑病毒 被引量:16
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作者 朱水芳 相宁 +2 位作者 张成良 A.Hadidi 裘维蕃 《中国进出境动植检》 1995年第4期29-31,共3页
利用根据ToRSV加拿大悬钩子株系核酸序列设计、合成的寡核苷酸引物进行PCR扩增试验,能成功地扩增ToRSV悬钩子株系的目标片段,但不能扩增苹果、樱桃株系的目标片段。 而ToRSV苹果株系的克隆pS_(64),经EcoRI酶切,再分别用^(32)P—UTP、Dig... 利用根据ToRSV加拿大悬钩子株系核酸序列设计、合成的寡核苷酸引物进行PCR扩增试验,能成功地扩增ToRSV悬钩子株系的目标片段,但不能扩增苹果、樱桃株系的目标片段。 而ToRSV苹果株系的克隆pS_(64),经EcoRI酶切,再分别用^(32)P—UTP、Dig—11—UTP和SP_6RNA多聚酶转录出^(32)P标记、Dig标记的cRNA探针后,不仅能有效地检测出ToRSV的苹果株系,而且能有效地检测樱桃株系和悬钩子株系。^(32)P与Dig—标记的探针灵敏度基本相同,而Dig标记的探针无放射性,可以长期保存,多次使用,灵敏、准确、安全、经济,是值得推广应用的检疫新方法。 展开更多
关键词 番茄 环斑病毒 探针检测
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Recent developments and trends in thermal blanching-A comprehensive review 被引量:30
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作者 Hong-Wei Xiao Zhongli Pan +5 位作者 Li-Zhen Deng Hamed M.El-Mashad Xu-Hai Yang Arun S.Mujumdar Zhen-Jiang Gao Qian Zhang 《Information Processing in Agriculture》 EI 2017年第2期101-127,共27页
Thermal blanching is an essential operation for many fruits and vegetables processing.It not only contributes to the inactivation of polyphenol oxidase(PPO),peroxidase(POD),but also affects other quality attributes of... Thermal blanching is an essential operation for many fruits and vegetables processing.It not only contributes to the inactivation of polyphenol oxidase(PPO),peroxidase(POD),but also affects other quality attributes of products.Herein we review the current status of thermal blanching.Firstly,the purposes of blanching,which include inactivating enzymes,enhancing drying rate and product quality,removing pesticide residues and toxic constituents,expelling air in plant tissues,decreasing microbial load,are examined.Then,the reason to why indicators such as POD and PPO,ascorbic acid,color,and texture are frequently used to evaluate blanching process is summarized.After that,the principles,applications and limitations of current thermal blanching methods,which include conventional hot water blanching,steam blanching,microwave blanching,ohmic blanching,and infrared blanching are outlined.Finally,future trends are identified and discussed. 展开更多
关键词 Thermal blanching Hot water blanching Microwave blanching Steam blanching Ohmic blanching Infrared blanching
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Rapid Identification of Rice Samples Using an Electronic Nose 被引量:28
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作者 John Westbrook W.C.Hoffmann R.E.Lacey 《Journal of Bionic Engineering》 SCIE EI CSCD 2009年第3期290-297,共8页
Four rice samples of long grain type were tested using an electronic nose (Cyranose-320).Samples of 5 g of each variety of rice were placed individually in vials and were analyzed with the electronic nose unit consist... Four rice samples of long grain type were tested using an electronic nose (Cyranose-320).Samples of 5 g of each variety of rice were placed individually in vials and were analyzed with the electronic nose unit consisting of 32 polymer sensors.The Cyranose-320 was able to differentiate between varieties of rice.The chemical composition of the rice odors for differentiating rice samples needs to be investigated.The optimum parameter settings should be considered during the Cyranose-320 training process especially for multiple samples,which are helpful for obtaining an accurate training model to improve identification capability.Further,it is necessary to investigate the E-nose sensor selection for obtaining better classification accuracy.A re- duced number of sensors could potentially shorten the data processing time,and could be used to establish an application pro- cedure and reduce the cost for a specific electronic nose.Further research is needed for developing analytical procedures that adapt the Cyranose-320 as a tool for testing rice quality. 展开更多
关键词 rice grain IDENTIFICATION electronic nose data analysis pattern recognition
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