Seaweeds possess a wide range of bioactive compounds which have been play a vital role in production of novel pharmaceutical and nutraceutical products resources.The present study employed in silico molecular docking ...Seaweeds possess a wide range of bioactive compounds which have been play a vital role in production of novel pharmaceutical and nutraceutical products resources.The present study employed in silico molecular docking to characterize the biomedical potential of brown seaweed Lobophora variegata through antibacterial,antibiofilm,antioxidant and anticancer activities.The antibacterial activity of L.variegata extracts was analyzed against Escherichia coli,Enterococcus faecalis,Staphylococcus aureus,and Pseudomonas aeruginosa strains producing biofilms.The antioxidant activity was assessed using(2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)),superoxide,and hydroxyl radical scavenging assays.Further,the anticancer,antibacterial,and antioxidant activities of the bioactive compounds were evaluated through molecular docking using GLIDE ligand docking module and prime MM-GBSA module in the Schrodinger software.The phytochemical analysis of the methanolic extract of L.variegata found more phycocompounds which was confirmed by FT-IR and GC-MS.The significant level of phenol and flavonoid were expressed in methanolic extract of L.variegata.In addition,the potent antioxidant activity of the methanolic extract was efficiently expressed during the free radical scavenging assays of ABTS,hydroxyl,and superoxide denoted as 34.94,26.64,and 29.86 mg/g,respectively.The antibacterial and biofilm activities of the methanolic extract of L.variegata had significant results.Moreover,the methanolic extract exhibited cytotoxicity against human colon cancer HT-29 cell lines at an inhibitory concentration is 193.65μg/mL.Through the computational analysis,glyceryl monostearate,2-palmitoylglycerol,phthalic acid,2-chloropropyl ethyl ester,phthalic acid,5-methylhex-2-yl ethyl ester,and l-(+)-ascorbic acid 2,6-dihexadecanoate results as top ranked molecules and shown to be a promising compounds,which were evaluated for anticancer,antioxidant,and antibacterial properties against multi-target proteins.Based on the experimental results,L.v展开更多
Objective: To screen the differentially expressed gene in liver regeneration with cDNA array and gain insight of the pathogenesis of the acute hepatic failure. Methods: Acute liver failure model in Sprague-Dawley (SD)...Objective: To screen the differentially expressed gene in liver regeneration with cDNA array and gain insight of the pathogenesis of the acute hepatic failure. Methods: Acute liver failure model in Sprague-Dawley (SD) rat was induced by 95% hepatectomy. The differential expression profiles in regenerating rat liver were studied by a cDNA array representing 1176 cDNA clusters. Some genes were randomly selected from a pool of differentially expressed genes and subjected to RT-PCR to further confirm the result from microarray hybridization. Results: The alterations of transcription levels were noted in the expressions of 188 genes. There were 138 genes with their expression up-regulated such as growth factors, PCNA, ribosomal proteins, IL6 and CDKs which were associated with cell cycle regulation, stress, metabolism and proliferation. Conclusion: cDNA array is a powerful tool to explore the gene expression of acute liver failure and is potential for the diagnosis and treatment of liver regeneration.展开更多
文摘Seaweeds possess a wide range of bioactive compounds which have been play a vital role in production of novel pharmaceutical and nutraceutical products resources.The present study employed in silico molecular docking to characterize the biomedical potential of brown seaweed Lobophora variegata through antibacterial,antibiofilm,antioxidant and anticancer activities.The antibacterial activity of L.variegata extracts was analyzed against Escherichia coli,Enterococcus faecalis,Staphylococcus aureus,and Pseudomonas aeruginosa strains producing biofilms.The antioxidant activity was assessed using(2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)),superoxide,and hydroxyl radical scavenging assays.Further,the anticancer,antibacterial,and antioxidant activities of the bioactive compounds were evaluated through molecular docking using GLIDE ligand docking module and prime MM-GBSA module in the Schrodinger software.The phytochemical analysis of the methanolic extract of L.variegata found more phycocompounds which was confirmed by FT-IR and GC-MS.The significant level of phenol and flavonoid were expressed in methanolic extract of L.variegata.In addition,the potent antioxidant activity of the methanolic extract was efficiently expressed during the free radical scavenging assays of ABTS,hydroxyl,and superoxide denoted as 34.94,26.64,and 29.86 mg/g,respectively.The antibacterial and biofilm activities of the methanolic extract of L.variegata had significant results.Moreover,the methanolic extract exhibited cytotoxicity against human colon cancer HT-29 cell lines at an inhibitory concentration is 193.65μg/mL.Through the computational analysis,glyceryl monostearate,2-palmitoylglycerol,phthalic acid,2-chloropropyl ethyl ester,phthalic acid,5-methylhex-2-yl ethyl ester,and l-(+)-ascorbic acid 2,6-dihexadecanoate results as top ranked molecules and shown to be a promising compounds,which were evaluated for anticancer,antioxidant,and antibacterial properties against multi-target proteins.Based on the experimental results,L.v
文摘Objective: To screen the differentially expressed gene in liver regeneration with cDNA array and gain insight of the pathogenesis of the acute hepatic failure. Methods: Acute liver failure model in Sprague-Dawley (SD) rat was induced by 95% hepatectomy. The differential expression profiles in regenerating rat liver were studied by a cDNA array representing 1176 cDNA clusters. Some genes were randomly selected from a pool of differentially expressed genes and subjected to RT-PCR to further confirm the result from microarray hybridization. Results: The alterations of transcription levels were noted in the expressions of 188 genes. There were 138 genes with their expression up-regulated such as growth factors, PCNA, ribosomal proteins, IL6 and CDKs which were associated with cell cycle regulation, stress, metabolism and proliferation. Conclusion: cDNA array is a powerful tool to explore the gene expression of acute liver failure and is potential for the diagnosis and treatment of liver regeneration.
