AIM: Ghrelin, an endogenous ligand for growth hormone secretagogue receptor, influences appetite, energy balance, gastric motility and acid secretion. The stomach is the main source of circulating ghrelin. There are i...AIM: Ghrelin, an endogenous ligand for growth hormone secretagogue receptor, influences appetite, energy balance, gastric motility and acid secretion. The stomach is the main source of circulating ghrelin. There are inconsistent reports on the influence of Helicobacter pylori (H pylori) infection on circulating ghrelin levels. We sought to elucidate the relationship between ghrelin and various peptides in plasma, with special reference to H pylori.METHODS: Plasma ghrelin levels were measured by radioimmunoassay in 89 subjects who were referred for upper gastrointestinal endoscopy, consisting of 42 H pylori infected and 47 uninfected ones. Plasma gastrin,somatostatin, leptin, insulin-like growth hormone 1 (IGF-1)and chromogranin A concentrations were also measured.Twelve patients were treated with anti- H pylori regimen.RESULTS: Ghrelin circulating levels were greatly decreased in H pylori-positive than negative individuals (194.2±90.2fmol/mL and 250.4±84.1 respectively, P＜0.05), but did not significantly alter following the cure of infection (176.5±79.5 vs 191.3±120.4). There was a significant negative correlation between circulating ghrelin and leptin levels, as well as body mass index, for the whole and uninfected population, but not in H pylori-infected patients. Plasma ghrelin concentrations correlated positively with IGF-1 in H pylori-negative group and negatively with chromogranin A in the infected group.There were no significant correlations among circulating levels of ghrelin, gastrin and somatostatin irrespective of H pylori status.CONCLUSION: H pylori infection influences plasma ghrelin dynamics and its interaction with diverse bioactive peptides involved in energy balance, growth and neuroendocrine function.展开更多
AIM: To determine the concentration of α-and β-defensins in gastric juice of patients with various gastroduodenal diseases. METHODS: Concentrations of human neutrophil peptides (HNPs) 1-3, the major forms of α-defe...AIM: To determine the concentration of α-and β-defensins in gastric juice of patients with various gastroduodenal diseases. METHODS: Concentrations of human neutrophil peptides (HNPs) 1-3, the major forms of α-defensins, and human β-defensin (HBD)-1and HBD-2were measured by radioimmunoassay in plasma and gastric juice of 84 subjects, consisting of 54 Helicobacter pylori-infected and 30 uninfected subjects. They included 33 patients with chronic gastritis (CG), 12 with gastric ulcer (GU), 11 with duodenal ulcer (DU), 11 with benign gastric polyp (BGP) and 16 with normal mucosa (N group) on upper endoscopy. Plasma pepsinogen Ⅰ and Ⅱ levels, biomarkers for gastric mucosal inflammation and atrophy, were also measured. RESULTS: Gastric juice HNPs 1-3 levels in patients with CG, GU and BGP were significantly higher than those in patients with DU and N. Gastric juice HBD-2 concentrations in patients with CG and GU were significantly higher than those in the N group, but were significantly lower in DU patients than in GU patients. Gastric juice HBD-1 levels and plasma levels of these peptides were similar in the patient groups. Concentrations of gastric juice HNPs 1-3 and HBD-2 of in H pylori-infected patients were significantly different from those in uninfected subjects. HNPs 1-3 concentrations in gastric juice correlated negatively with plasma pepsinogen I levels and Ⅰ/Ⅱ ratios. HBD-2 levels in gastric juice correlated positively and negatively with plasma pepsinogen Ⅱ concentrations and Ⅰ/Ⅱratios, respectively. CONCLUSION: HNPs 1-3 and HBD-2 levels in gastric juice are diverse among various gastrointestinal diseases, reflecting the inflammatory and atrophic events of the background gastric mucosa affected by H pylori.展开更多
Gene targeting of mouse homolog of interleukin-8 receptor (IL-8R) resulted in the increase of myeloid cells in the bone marrow and peripheral blood, sugesting a potential ro le of IL-8 mregulating hematopoiesis of the...Gene targeting of mouse homolog of interleukin-8 receptor (IL-8R) resulted in the increase of myeloid cells in the bone marrow and peripheral blood, sugesting a potential ro le of IL-8 mregulating hematopoiesis of the myeloid lineage.In order to clarify the functions of IL-8 inhematopoiesis,展开更多
To investigate the expression of apoptosis-related protein(Fas,FasL,and Bcl-2)in the pathogenesis of autoimmune thyroid disorders (ATDs),immunohistochemical staining was performed on 20 Hashimoto's thyroiditis(HT)...To investigate the expression of apoptosis-related protein(Fas,FasL,and Bcl-2)in the pathogenesis of autoimmune thyroid disorders (ATDs),immunohistochemical staining was performed on 20 Hashimoto's thyroiditis(HT),20 Graves'disease(GD),and 20 thyroid follicular adenoma(TFA,as control).All the cases expressed Fas,mainly on the cell surface and cytoplasm.FasL was found in 17 cases of the TFA.Bcl-2 was detected in 15 cases of HT,19 of GD and 17 of TFA.In TFA,a moderate Fas expression and a minimal or no FasL expression was detected on follicular cells.In HT,the follicles adjacent to infiltrating lymphocytes showed increased levels of Fas and FasL expression.A weaker staining of Fas and FasL was exhibited on infiltrating lymphocytes than on thyrocytes.In a comparison of GD with HT,thyrocytes and lymphocytes showed similar Fas staining,but for FasL the staining was rather weaker in HT.The expression of Bcl-2 was nearly identical in GD and TFA,but much weaker on the follicular cells in vicinity of lymphocytes and on the lymphocytes located in germinal centers of HT tissues.The expression of Fas,FasL,Bcl-2 in Hashimoto's thyroiditis and Graves' disease were almost same.FasL strong expression and Bcl-2 weak expression on the follicles in HT may induce apoptosis.These results provided evidence for expression of Fas,FasL and Bcl-2 in the pathogenesis of autoimmune thyroid disease.The lymphocytes seem not to be directly engaged in the process via their own FasL,but they may provide some cytokines that,in turn,upregulate Fas and/or FasL expression to induce apoptosis.Cellular & Molecular Immunology.2004;1(3):224-228.展开更多
all diabetic patients should be screened for the early stage of diabetic nephropathy,because microalbuminuria has a great prognostic significance.The albusure test(AT),alatex agglutination nephelometric immunoassay,is...all diabetic patients should be screened for the early stage of diabetic nephropathy,because microalbuminuria has a great prognostic significance.The albusure test(AT),alatex agglutination nephelometric immunoassay,is a rapid and low cost test for the detection of microalbuminurai of 30mg/L or more.展开更多
文摘AIM: Ghrelin, an endogenous ligand for growth hormone secretagogue receptor, influences appetite, energy balance, gastric motility and acid secretion. The stomach is the main source of circulating ghrelin. There are inconsistent reports on the influence of Helicobacter pylori (H pylori) infection on circulating ghrelin levels. We sought to elucidate the relationship between ghrelin and various peptides in plasma, with special reference to H pylori.METHODS: Plasma ghrelin levels were measured by radioimmunoassay in 89 subjects who were referred for upper gastrointestinal endoscopy, consisting of 42 H pylori infected and 47 uninfected ones. Plasma gastrin,somatostatin, leptin, insulin-like growth hormone 1 (IGF-1)and chromogranin A concentrations were also measured.Twelve patients were treated with anti- H pylori regimen.RESULTS: Ghrelin circulating levels were greatly decreased in H pylori-positive than negative individuals (194.2±90.2fmol/mL and 250.4±84.1 respectively, P＜0.05), but did not significantly alter following the cure of infection (176.5±79.5 vs 191.3±120.4). There was a significant negative correlation between circulating ghrelin and leptin levels, as well as body mass index, for the whole and uninfected population, but not in H pylori-infected patients. Plasma ghrelin concentrations correlated positively with IGF-1 in H pylori-negative group and negatively with chromogranin A in the infected group.There were no significant correlations among circulating levels of ghrelin, gastrin and somatostatin irrespective of H pylori status.CONCLUSION: H pylori infection influences plasma ghrelin dynamics and its interaction with diverse bioactive peptides involved in energy balance, growth and neuroendocrine function.
文摘AIM: To determine the concentration of α-and β-defensins in gastric juice of patients with various gastroduodenal diseases. METHODS: Concentrations of human neutrophil peptides (HNPs) 1-3, the major forms of α-defensins, and human β-defensin (HBD)-1and HBD-2were measured by radioimmunoassay in plasma and gastric juice of 84 subjects, consisting of 54 Helicobacter pylori-infected and 30 uninfected subjects. They included 33 patients with chronic gastritis (CG), 12 with gastric ulcer (GU), 11 with duodenal ulcer (DU), 11 with benign gastric polyp (BGP) and 16 with normal mucosa (N group) on upper endoscopy. Plasma pepsinogen Ⅰ and Ⅱ levels, biomarkers for gastric mucosal inflammation and atrophy, were also measured. RESULTS: Gastric juice HNPs 1-3 levels in patients with CG, GU and BGP were significantly higher than those in patients with DU and N. Gastric juice HBD-2 concentrations in patients with CG and GU were significantly higher than those in the N group, but were significantly lower in DU patients than in GU patients. Gastric juice HBD-1 levels and plasma levels of these peptides were similar in the patient groups. Concentrations of gastric juice HNPs 1-3 and HBD-2 of in H pylori-infected patients were significantly different from those in uninfected subjects. HNPs 1-3 concentrations in gastric juice correlated negatively with plasma pepsinogen I levels and Ⅰ/Ⅱ ratios. HBD-2 levels in gastric juice correlated positively and negatively with plasma pepsinogen Ⅱ concentrations and Ⅰ/Ⅱratios, respectively. CONCLUSION: HNPs 1-3 and HBD-2 levels in gastric juice are diverse among various gastrointestinal diseases, reflecting the inflammatory and atrophic events of the background gastric mucosa affected by H pylori.
文摘Gene targeting of mouse homolog of interleukin-8 receptor (IL-8R) resulted in the increase of myeloid cells in the bone marrow and peripheral blood, sugesting a potential ro le of IL-8 mregulating hematopoiesis of the myeloid lineage.In order to clarify the functions of IL-8 inhematopoiesis,
文摘To investigate the expression of apoptosis-related protein(Fas,FasL,and Bcl-2)in the pathogenesis of autoimmune thyroid disorders (ATDs),immunohistochemical staining was performed on 20 Hashimoto's thyroiditis(HT),20 Graves'disease(GD),and 20 thyroid follicular adenoma(TFA,as control).All the cases expressed Fas,mainly on the cell surface and cytoplasm.FasL was found in 17 cases of the TFA.Bcl-2 was detected in 15 cases of HT,19 of GD and 17 of TFA.In TFA,a moderate Fas expression and a minimal or no FasL expression was detected on follicular cells.In HT,the follicles adjacent to infiltrating lymphocytes showed increased levels of Fas and FasL expression.A weaker staining of Fas and FasL was exhibited on infiltrating lymphocytes than on thyrocytes.In a comparison of GD with HT,thyrocytes and lymphocytes showed similar Fas staining,but for FasL the staining was rather weaker in HT.The expression of Bcl-2 was nearly identical in GD and TFA,but much weaker on the follicular cells in vicinity of lymphocytes and on the lymphocytes located in germinal centers of HT tissues.The expression of Fas,FasL,Bcl-2 in Hashimoto's thyroiditis and Graves' disease were almost same.FasL strong expression and Bcl-2 weak expression on the follicles in HT may induce apoptosis.These results provided evidence for expression of Fas,FasL and Bcl-2 in the pathogenesis of autoimmune thyroid disease.The lymphocytes seem not to be directly engaged in the process via their own FasL,but they may provide some cytokines that,in turn,upregulate Fas and/or FasL expression to induce apoptosis.Cellular & Molecular Immunology.2004;1(3):224-228.
文摘all diabetic patients should be screened for the early stage of diabetic nephropathy,because microalbuminuria has a great prognostic significance.The albusure test(AT),alatex agglutination nephelometric immunoassay,is a rapid and low cost test for the detection of microalbuminurai of 30mg/L or more.
