The relationship between Solidago canadensis L. invasion and soil microbial communities was studied across the invasive gradients varying from 0 to 40, 80, and 100% coverage of S. canadensis. The results showed both s...The relationship between Solidago canadensis L. invasion and soil microbial communities was studied across the invasive gradients varying from 0 to 40, 80, and 100% coverage of S. canadensis. The results showed both soil microbial biomass C （Cmic） and N （Nmic） increased as the coverage of S. canadensis increased. Soil microbial quotient Cmic/Corg （microbial biomass C/organic C） tended to increase linearly with the coverage of S. canadensis. Soil basal respiration （BR） also showed a similar trend. The soil respiratory quotient qCO2 decreased with S. canadensis invasion, and remained at quite a constantly low level in the invasive soils. Sole carbon source utilization profiles analyses indicated that S. canadensis invasion tended to result in higher microbial functional diversity in the soil. Average utilization of specific substrate guilds was highest in the soil with S. canadensis monoculture. Principle component analysis of sole carbon source utilization profiles further indicated that microbial functional diversity in the soil with S. canadensis monoculture was distinctly separated from those soils in the native area and the ecotones. In conclusion, S. canadensis invasion improved soil microbial biomass, respiration and utilization of carbon sources, and decreased qCO2, thus created better soil conditions, which in turn were more conducive to the growth of S. canadensis.展开更多
Objective To establish a lung cancer dormancy mouse model and verify the effects of Wushen(WS),a formula food,on postoperative recurrence.Methods We established a Lewis cell tumor dormancy model system that definitive...Objective To establish a lung cancer dormancy mouse model and verify the effects of Wushen(WS),a formula food,on postoperative recurrence.Methods We established a Lewis cell tumor dormancy model system that definitively links surgery and the subsequent wound-healing response to the outgrowth of lung cancer cells.We used this model to observe the effects of WS on the postoperative recurrence and the nutritional status of the mice.Finally,the immunocyte subtypes and cytokine levels in the serum and spleens of mice were detected by flow cytometry and ELISA.Results The recurrence rate in the WS group was obviously lower than that in the control group.Wushen increased the body weights and serum albumin levels of the mice.The levels of NK,Gr1+CD11b+CD3+CD8+and CD3+CD4+T cells in the spleens of mice in the WS group were also increased.Compared with the control group,the levels of CD4+IFN-γ+,CD4+IL-2 and CD4+/IL-10+in the spleens of mice in the WS group were decreased.Wushen also seemed to decrease the levels of IL-6 and TNF-α,but the decrease was not significant.Conclusion The postoperative lung cancer recurrence model was successfully established.Wushen inhibited postoperative recurrence,apparently by regulating the level of immune cell subtypes and cytokines in the serum and spleen.展开更多
基金supported by the Fundamental Research Funds for the Central Universities of China (2009QNA6015)Foundation of Zhejiang Educational Committee of China (Y200803219)
文摘The relationship between Solidago canadensis L. invasion and soil microbial communities was studied across the invasive gradients varying from 0 to 40, 80, and 100% coverage of S. canadensis. The results showed both soil microbial biomass C （Cmic） and N （Nmic） increased as the coverage of S. canadensis increased. Soil microbial quotient Cmic/Corg （microbial biomass C/organic C） tended to increase linearly with the coverage of S. canadensis. Soil basal respiration （BR） also showed a similar trend. The soil respiratory quotient qCO2 decreased with S. canadensis invasion, and remained at quite a constantly low level in the invasive soils. Sole carbon source utilization profiles analyses indicated that S. canadensis invasion tended to result in higher microbial functional diversity in the soil. Average utilization of specific substrate guilds was highest in the soil with S. canadensis monoculture. Principle component analysis of sole carbon source utilization profiles further indicated that microbial functional diversity in the soil with S. canadensis monoculture was distinctly separated from those soils in the native area and the ecotones. In conclusion, S. canadensis invasion improved soil microbial biomass, respiration and utilization of carbon sources, and decreased qCO2, thus created better soil conditions, which in turn were more conducive to the growth of S. canadensis.
文摘Objective To establish a lung cancer dormancy mouse model and verify the effects of Wushen(WS),a formula food,on postoperative recurrence.Methods We established a Lewis cell tumor dormancy model system that definitively links surgery and the subsequent wound-healing response to the outgrowth of lung cancer cells.We used this model to observe the effects of WS on the postoperative recurrence and the nutritional status of the mice.Finally,the immunocyte subtypes and cytokine levels in the serum and spleens of mice were detected by flow cytometry and ELISA.Results The recurrence rate in the WS group was obviously lower than that in the control group.Wushen increased the body weights and serum albumin levels of the mice.The levels of NK,Gr1+CD11b+CD3+CD8+and CD3+CD4+T cells in the spleens of mice in the WS group were also increased.Compared with the control group,the levels of CD4+IFN-γ+,CD4+IL-2 and CD4+/IL-10+in the spleens of mice in the WS group were decreased.Wushen also seemed to decrease the levels of IL-6 and TNF-α,but the decrease was not significant.Conclusion The postoperative lung cancer recurrence model was successfully established.Wushen inhibited postoperative recurrence,apparently by regulating the level of immune cell subtypes and cytokines in the serum and spleen.
