AIM: To investigate the inhibitory effect of a Chinese herb medicine Astragali radix (AR) on growth of different cancer cell lines.METHODS: To observe the in vitro effects of AR on tumor cell proliferation by trypan b...AIM: To investigate the inhibitory effect of a Chinese herb medicine Astragali radix (AR) on growth of different cancer cell lines.METHODS: To observe the in vitro effects of AR on tumor cell proliferation by trypan blue exclusion, MTS method and tritium thymidine incorporation assay. Apoptosis was detected by DNA ladder method.RESULTS: The inhibition rates of AR on the cell respiration of AGS, KATOⅢ, HT29, MDA231, MEL7 and MEL14 were 68.25％, 62.36％, 22.8％, 27.69％, 2.85％ and 5.14％respectively at the concentration of 100 ug/ml; it inhibited AGS DNA synthesis by 87.33％ at the concentration of 50ug/ml. The inhibitory effect on AGS was time-and dosedependent. AR did not induce apoptosis in AGS cells.CONCLUSION: AR specifically inhibits gastric cancer cells growth in vitro and the mechanism is mainly cytostatic but not cytotoxic or inducing apoptosis.展开更多
Resveratrol (3, 5, 4'-trihydroxystilbene) (RV) is a constituent of grape seeds with anti-inflammatory andanti-oxidant activities. In this study, we examined the capacity of RV to modulate the function of Gprotein-...Resveratrol (3, 5, 4'-trihydroxystilbene) (RV) is a constituent of grape seeds with anti-inflammatory andanti-oxidant activities. In this study, we examined the capacity of RV to modulate the function of Gprotein-coupled chemoattractant receptors, which play important roles in inflammation and immune responses.RV, over a non-cytotoxic concentration range, inhibited chemotactic and calcium mobilization responses ofphagocytic cells to selected chemoattractants. At low micromolar concentrations,RV potently reducedsuperoxide anion production by phagocytic leukocytes in response to the bacterial chemotactic peptide fMLF, ahigh affinity ligand for formylpeptide receptor FPR, and Ap.4z, an Alzheimer's disease-associated peptide and aligand for the FPR variant FPRL1. In addition, RV reduced phosphorylation of extracellular signal-regulatedkinase (ERK1/2) and the activation of nuclear factor NF-KB induced by formylpeptide receptor agonists. Theseresults suggest that the inhibition of the function of chemoattractant receptors may contribute to theanti-inflammatory properties of RV Thus, RV may be therapeutically promising for diseases in which activationof formylpeptide receptors contributes to the pathogenic processes. Cellular&Molecular Immunology. 2004;1(1):50-56.展开更多
Objective: The aim in this study was to identify the allelicfrequencies of the chemokine (SDF1-3'A) and chemokinereceptor (CCR5△32, CCR5m303 and CCR2-64I) genesresistant to HIV-1 infection and/or disease progress...Objective: The aim in this study was to identify the allelicfrequencies of the chemokine (SDF1-3'A) and chemokinereceptor (CCR5△32, CCR5m303 and CCR2-64I) genesresistant to HIV-1 infection and/or disease progression inindigenous Chinese populations. Methods: By using QIAamp DNA Blood Mini Kit, thegenomic DNA samples were purified from whole peripheralblood of healthy individuals (n=2067) from Han, Uygur,Mongolian and Tibetan ethnic groups, as well as Han patientsincluding HIV-1 carriers (n=330), patients with other sexuallytransmitted diseases (STDs, n=259) and intravenous drugusers (IVDUs, n=125). The allelic polymorphisms wereidentified by means of PCR or PCR-RFLP analyses. Thesequences of randomly selected amplified PCR products werefurther confirmed by direct DNA sequencing. Results: The mutant frequencies were identified to be0%～3.48% for CCR5△32, 0% for CCR5m303,19.15%～28.79% for CCR2-64 and 19.10%～28.73% for SDF1-3'A alleles, respectively, in Chinese healthy individuals fromfour ethnic groups. Our findings indicated the allelicfrequencies vary among the different ethnic groups.Furthermore, the HIV-1 carriers, STD cases and IVDUs (all ofHan ethnicity) were found to have the allelic frequencies of0%～0.19% (CCR5△32), 0% (CCR5m303), 19.31%～20.45%(CCR2-64) and 25.61%～26.83% (SDF1-3'A) with minorvariations in their frequencies between the patients andhealthy Han groups. There was no CCR5-m303 mutationfound in any subject in this study. Conclusion: The examined subjects of four Chinese ethnicorigins showed lower frequencies of CCR5△32 andCCR5m303 alleles, but higher frequencies of mutant CCR2-64I and SDF1-3'A alleles compared to those identified innorthern-European and American Caucasians. Thesignificance of the different frequencies and polymorphisms ofthe above alleles in Chinese populations needs to be furtherexamined in HIV-1/AIDS diseases.展开更多
Chemokines belong to a large family of inflammatory cytokines responsible for migration and accumulation of leukocytes at inflammatory sites.Over the past decade,accumulating evidence indicated a crucial role for chem...Chemokines belong to a large family of inflammatory cytokines responsible for migration and accumulation of leukocytes at inflammatory sites.Over the past decade,accumulating evidence indicated a crucial role for chemokines and chemokine receptors in the pathophysiology of rheumatoid arthritis(RA).RA is a chronic autoimmune disease in which the synovial tissue is heavily infiltrated by leukocytes.Chemokines play an important role in the infiltration,localization,retention of infiltrating leukocytes and generation of ectopic germinal centers in the inflamed synovium.Recent evidence also suggests that identification of inhibitors directly targeting chemokines or their receptors may provide a novel therapeutic strategy in RA.Traditional Chinese medicines(TCMs) have a long history in the treatment of inflammatory joint disease.The basis for the clinical benefits of TCM remains largely unclear.Our studies have led to the identification of numerous novel chemokine/chemokine receptor inhibitors present in anti-inflammatory TCMs.All of these inhibitors were previously reported by other researchers to have anti-arthritic effect,which may be attributable,at least in part,to their inhibitory effect on chemokine and/or chemokine receptor.Therefore,identification of agents capable of targeting chemokine/chemokine receptor interactions has suggested a mechanism of action for several TCM components and provided a means of identifying additional anti-RA TCM.Thus,this approach may lead to the discovery of new inhibitors of chemokines or chemokine receptors that can be used to treat diseases associated with inappropriately overactive chemokine mediated inflammatory reactions.Cellular & Molecular Immunology.2004;1(5):336-342.展开更多
The nuclear receptor superfamily and the transcriptional factors associated with cytokines are inherently different families of signaling molecules and activate gene transcription by binding to their respective respon...The nuclear receptor superfamily and the transcriptional factors associated with cytokines are inherently different families of signaling molecules and activate gene transcription by binding to their respective responsive element.However,it has become increasingly clear from our works and others that nuclear receptors are important regulators of cytokine production and function through complex and varied interactions between these distinct transcriptional factors.This review provides a general overview of the mechanism of action of nuclear receptors and their transcriptional crosstalk with transcriptional factors associated with cytokine transduction pathways.One of the most important mechanistic aspects is protein to protein interaction through a direct or co-regulator-mediated indirect manner.Such crosstalk is crucially involved in physiological and therapeutic roles of nuclear receptors and their iigands in immunity, inflammation and cytokine-related tumors.Cellular & Molecular Immunology.2004;1(6):416-424.展开更多
文摘AIM: To investigate the inhibitory effect of a Chinese herb medicine Astragali radix (AR) on growth of different cancer cell lines.METHODS: To observe the in vitro effects of AR on tumor cell proliferation by trypan blue exclusion, MTS method and tritium thymidine incorporation assay. Apoptosis was detected by DNA ladder method.RESULTS: The inhibition rates of AR on the cell respiration of AGS, KATOⅢ, HT29, MDA231, MEL7 and MEL14 were 68.25％, 62.36％, 22.8％, 27.69％, 2.85％ and 5.14％respectively at the concentration of 100 ug/ml; it inhibited AGS DNA synthesis by 87.33％ at the concentration of 50ug/ml. The inhibitory effect on AGS was time-and dosedependent. AR did not induce apoptosis in AGS cells.CONCLUSION: AR specifically inhibits gastric cancer cells growth in vitro and the mechanism is mainly cytostatic but not cytotoxic or inducing apoptosis.
文摘Resveratrol (3, 5, 4'-trihydroxystilbene) (RV) is a constituent of grape seeds with anti-inflammatory andanti-oxidant activities. In this study, we examined the capacity of RV to modulate the function of Gprotein-coupled chemoattractant receptors, which play important roles in inflammation and immune responses.RV, over a non-cytotoxic concentration range, inhibited chemotactic and calcium mobilization responses ofphagocytic cells to selected chemoattractants. At low micromolar concentrations,RV potently reducedsuperoxide anion production by phagocytic leukocytes in response to the bacterial chemotactic peptide fMLF, ahigh affinity ligand for formylpeptide receptor FPR, and Ap.4z, an Alzheimer's disease-associated peptide and aligand for the FPR variant FPRL1. In addition, RV reduced phosphorylation of extracellular signal-regulatedkinase (ERK1/2) and the activation of nuclear factor NF-KB induced by formylpeptide receptor agonists. Theseresults suggest that the inhibition of the function of chemoattractant receptors may contribute to theanti-inflammatory properties of RV Thus, RV may be therapeutically promising for diseases in which activationof formylpeptide receptors contributes to the pathogenic processes. Cellular&Molecular Immunology. 2004;1(1):50-56.
基金This project was supported by grant from National Natural Sciences Foundation of the PR China(39770683)
文摘Objective: The aim in this study was to identify the allelicfrequencies of the chemokine (SDF1-3'A) and chemokinereceptor (CCR5△32, CCR5m303 and CCR2-64I) genesresistant to HIV-1 infection and/or disease progression inindigenous Chinese populations. Methods: By using QIAamp DNA Blood Mini Kit, thegenomic DNA samples were purified from whole peripheralblood of healthy individuals (n=2067) from Han, Uygur,Mongolian and Tibetan ethnic groups, as well as Han patientsincluding HIV-1 carriers (n=330), patients with other sexuallytransmitted diseases (STDs, n=259) and intravenous drugusers (IVDUs, n=125). The allelic polymorphisms wereidentified by means of PCR or PCR-RFLP analyses. Thesequences of randomly selected amplified PCR products werefurther confirmed by direct DNA sequencing. Results: The mutant frequencies were identified to be0%～3.48% for CCR5△32, 0% for CCR5m303,19.15%～28.79% for CCR2-64 and 19.10%～28.73% for SDF1-3'A alleles, respectively, in Chinese healthy individuals fromfour ethnic groups. Our findings indicated the allelicfrequencies vary among the different ethnic groups.Furthermore, the HIV-1 carriers, STD cases and IVDUs (all ofHan ethnicity) were found to have the allelic frequencies of0%～0.19% (CCR5△32), 0% (CCR5m303), 19.31%～20.45%(CCR2-64) and 25.61%～26.83% (SDF1-3'A) with minorvariations in their frequencies between the patients andhealthy Han groups. There was no CCR5-m303 mutationfound in any subject in this study. Conclusion: The examined subjects of four Chinese ethnicorigins showed lower frequencies of CCR5△32 andCCR5m303 alleles, but higher frequencies of mutant CCR2-64I and SDF1-3'A alleles compared to those identified innorthern-European and American Caucasians. Thesignificance of the different frequencies and polymorphisms ofthe above alleles in Chinese populations needs to be furtherexamined in HIV-1/AIDS diseases.
文摘Chemokines belong to a large family of inflammatory cytokines responsible for migration and accumulation of leukocytes at inflammatory sites.Over the past decade,accumulating evidence indicated a crucial role for chemokines and chemokine receptors in the pathophysiology of rheumatoid arthritis(RA).RA is a chronic autoimmune disease in which the synovial tissue is heavily infiltrated by leukocytes.Chemokines play an important role in the infiltration,localization,retention of infiltrating leukocytes and generation of ectopic germinal centers in the inflamed synovium.Recent evidence also suggests that identification of inhibitors directly targeting chemokines or their receptors may provide a novel therapeutic strategy in RA.Traditional Chinese medicines(TCMs) have a long history in the treatment of inflammatory joint disease.The basis for the clinical benefits of TCM remains largely unclear.Our studies have led to the identification of numerous novel chemokine/chemokine receptor inhibitors present in anti-inflammatory TCMs.All of these inhibitors were previously reported by other researchers to have anti-arthritic effect,which may be attributable,at least in part,to their inhibitory effect on chemokine and/or chemokine receptor.Therefore,identification of agents capable of targeting chemokine/chemokine receptor interactions has suggested a mechanism of action for several TCM components and provided a means of identifying additional anti-RA TCM.Thus,this approach may lead to the discovery of new inhibitors of chemokines or chemokine receptors that can be used to treat diseases associated with inappropriately overactive chemokine mediated inflammatory reactions.Cellular & Molecular Immunology.2004;1(5):336-342.
文摘The nuclear receptor superfamily and the transcriptional factors associated with cytokines are inherently different families of signaling molecules and activate gene transcription by binding to their respective responsive element.However,it has become increasingly clear from our works and others that nuclear receptors are important regulators of cytokine production and function through complex and varied interactions between these distinct transcriptional factors.This review provides a general overview of the mechanism of action of nuclear receptors and their transcriptional crosstalk with transcriptional factors associated with cytokine transduction pathways.One of the most important mechanistic aspects is protein to protein interaction through a direct or co-regulator-mediated indirect manner.Such crosstalk is crucially involved in physiological and therapeutic roles of nuclear receptors and their iigands in immunity, inflammation and cytokine-related tumors.Cellular & Molecular Immunology.2004;1(6):416-424.
