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小麦禾谷胞囊线虫(Heterodera avenae)的核糖体基因(rDNA)限制性片段长度多态性研究 被引量:46
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作者 彭德良 S.Subbotin M.Moens 《植物病理学报》 CAS CSCD 北大核心 2003年第4期323-329,共7页
采用PCR技术扩增出中国和摩洛哥禾谷胞囊线虫群体的核糖体基因 (rDNA)的内转录间隔区 (ITS)片段的长度约为 10 6 0bp。用 11种限制性内切酶 (RE)酶切禾谷胞囊线虫ITS扩增产物 ,共产生 2 7个酶切片段。用AluI和RsaI酶切ITS扩增产物证明... 采用PCR技术扩增出中国和摩洛哥禾谷胞囊线虫群体的核糖体基因 (rDNA)的内转录间隔区 (ITS)片段的长度约为 10 6 0bp。用 11种限制性内切酶 (RE)酶切禾谷胞囊线虫ITS扩增产物 ,共产生 2 7个酶切片段。用AluI和RsaI酶切ITS扩增产物证明中国禾谷胞囊线虫ITS属于“B型” ,而摩洛哥禾谷胞囊线虫ITS属于“A型”。用HinfI酶切后 ,7个中国禾谷胞囊线虫群体产生 2个RFLP片段 (86 0和 2 0 0bp) ,而摩洛哥群体产生 3个RFLP片段 (5 2 0、340和 2 0 0bp) ,HinfI揭示出中国与摩洛哥禾谷胞囊线虫ITS之间存在差异。AvaI和HindIII不能酶切禾谷胞囊线虫ITS。用CfoI、Bsh12 36I、MsrFI、ScrFI、HaeIII和MvaI 6种RE酶切中国和摩洛哥禾谷胞囊线虫群体的rDNA ITS ,均分别得到相同类型的RFLP分布型 ,因此这 6种RE不能揭示中国与摩洛哥群体的rDNA ITS的差异。 展开更多
关键词 小麦 禾谷胞囊线虫 Heterodera-avenae 核糖体基因 RDNA 限制性 片段长度 多态性
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Wheat stripe rust resistance gene Yr24/Yr26:A retrospective review 被引量:17
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作者 Robert McIntosh Jingmei Mu +1 位作者 Dejun Han Zhensheng Kang 《The Crop Journal》 SCIE CAS CSCD 2018年第4期321-329,共9页
The objective of this review is to describe events in China and elsewhere that are related to the discovery, genetic identification, use, and ultimate break-down of a single wheat gene for resistance to stripe rust, n... The objective of this review is to describe events in China and elsewhere that are related to the discovery, genetic identification, use, and ultimate break-down of a single wheat gene for resistance to stripe rust, namely Yr24/Yr26. In our retrospective analysis there was an early assumption of at least three genes at or near the locus, which caused an erroneous presumption of genetic diversity for resistance. It is an example of another boom and bust cycle in plant breeding with races virulent to Yr26(V26 races) now being the majority race group in the Chinese Pst population. We have attempted to present our story in a historical and personal context demonstrating research inputs from different national and international groups, as well as some significant contemporary side issues. It covers the period from the late 1980 s to 2017, during which significant rapid advances in the molecular biology of host: pathogen genetics occurred. We attempt to describe both successes and drawbacks in our work. 展开更多
关键词 Puccinia striiformis Resistance genes Triticum aestivum VIRULENCE Yellow rust
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High-efficiency and multiplex adenine base editing in plants using new TadA variants 被引量:15
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作者 Daqi Yan Bin Ren +6 位作者 Lang Liu Fang Yan Shaofang Li Guirong Wang Wenxian Sun Xueping Zhou Huanbin Zhou 《Molecular Plant》 SCIE CAS CSCD 2021年第5期722-731,共10页
Recently reported adenine base editors(ABEs)exhibit powerful potential for targeted gene correction as well as developing gain-of-function mutants and novel germplasms for both gene function studies and crop breeding.... Recently reported adenine base editors(ABEs)exhibit powerful potential for targeted gene correction as well as developing gain-of-function mutants and novel germplasms for both gene function studies and crop breeding.However,editing efficiency varies significantly among different target sites.Here,we investigated the activities of three evolved E.coli adenosine deaminase TadA variants(TadA8e,TadA8.17,and TadA8.20)side-by-side in transgenic rice.We found that TadA8e outperforms TadA8.17 and TadA8.20,and induces efficient A-to-G conversion at all tested sites in the rice genome,including those that were un-editable by ABE7.10 in our previous experiments.Furthermore,V82S/Q154R mutations were incorporated into TadA8e,resulting in a new variant that we named TadA9.Our data show that TadA9 is broadly compatible with CRISPR/SpCas9,CRISPR/SpCas9-NG,and CRISPR/SpRY,as well as CRISPR/ScCas9 nickase systems,achieving comparable or enhanced editing in a larger editing window at diverse PAM sites as compared with TadA8e.Finally,TadA9 was used to simultaneously install novel SNPs in four endogenous herbicide target genes in the commercial rice cultivar Nangeng 46 for potential field application in.weed control.Collectively,we successfully generated a series of novel ABEs that can efficiently edit adenosines in the rice genome.Our findings suggest that TadA9 and TadA8e have great potentials in the development of plant base editors and crop molecular breeding. 展开更多
关键词 CRISPR TadA variant adenine base editing Oryza sativa L.
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DEVELOPMENT OF COMMON WHEAT GERM-PLASM RESISTANT TO BARLEY YELLOW DWARF VIRUS BY BIOTECHNOLOGY 被引量:13
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作者 辛志勇 徐惠君 +8 位作者 陈孝 林志珊 周广和 钱幼亭 成卓敏 P. J. LARKIN P. BANKS R. APPELS B. GLARKE AND R. I. S. BRETTELL 《Science China Chemistry》 SCIE EI CAS 1991年第9期1055-1062,共8页
Barley yellow dwarf virus (BYDV) is one of the most serious wheat diseases in China. So far no resistance has been described in common wheat. A certain level of BYDV resistance was found in thirteen Triticeae species.... Barley yellow dwarf virus (BYDV) is one of the most serious wheat diseases in China. So far no resistance has been described in common wheat. A certain level of BYDV resistance was found in thirteen Triticeae species. Thinopyrum intermedium, two octoploids derived from TH. intermedium/wheat, Zhong 4 awnless and TAF46, and one disomic addition line, L1 derived from TAF46, showed good resistance to BYDV by enzyme linked immunosorbent assay (ELISA). Two wheat/TA. intermedium translocation lines, CPI 119880 and CPI 119899, showing good BYDV resistance were developed from L1 by using both CSph mutant and tissue culture. It is found that their BYDV resistance was controlled by a single dominant gene. Two cDNA probes pEleAcc3 and pPJN8 (E1-T1) were screened for detecting Th. intermedium DNA in wheat background. A specific band for the DNA of Th. intermedium and its derivatives was found in Southern hybridization. It is also possible to determine the size of the alien segment by comparing the relative density of the specific band. Therefore, this can be used as a marker to identify the BYDV resistance in wheat breeding program. 展开更多
关键词 wheat YELLOW DWARF VIRUS BARLEY YELLOW DWARF VIRUS Thinopyrum intermedium addition LINE TRANSLOCATION LINE cDNA probe.
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A new distinct geminivirus causes soybean stay-green disease 被引量:11
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作者 Ruixiang Cheng Ruoxin Mei +15 位作者 Rong Yan Hongyu Chen Dan Miao Lina Cai Jiayi Fan Gairu Li Ran Xu Weiguo Lu Yu Gao Wenwu Ye Shuo Su Tianfu Han Junyi Gai Yuanchao Wang Xiaorong Tao Yi Xu 《Molecular Plant》 SCIE CAS CSCD 2022年第6期927-930,共4页
Dear Editor,Plant viruses make up almost half of the plant disease-causing pathogens,affecting crop yields and the global economy(Savary et al.,2019).Soybean(Glycine max)is one of the most valuable legume crops in the... Dear Editor,Plant viruses make up almost half of the plant disease-causing pathogens,affecting crop yields and the global economy(Savary et al.,2019).Soybean(Glycine max)is one of the most valuable legume crops in the world,supplying 25%of the global edible oil and two-thirds of the global concentrated protein for livestock feeding.Recently,the outbreak of soybean stay-green syndrome with delayed leaf senescence(stay-green),flat pods,and increased number of abnormal seeds has swept the soybean production in the Huang-Huai-Hai region of China,resulting in huge yield losses(Xu et al.,2019).This disease has become an epidemic and prominent problem in soybean production and is still expanding its geography,including North America,posing a serious threat to soybean production(Harbach et al.,2016;Zhang et al.,2016;Li et al.,2019).However,the cause of soybean stay-green syndrome remains obscure. 展开更多
关键词 SOYBEAN CROPS EXPANDING
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Fungal diversity notes 1036-1150:taxonomic and phylogenetic contributions on genera and species of fungal taxa 被引量:8
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作者 Kevin D.Hyde Danushka S.Tennakoon +81 位作者 Rajesh Jeewon DJayarama Bhat Sajeewa S.N.Maharachchikumbura Walter Rossi Marco Leonardi Hyang Burm Lee Hye Yeon Mun Jos Houbraken Thuong T.T.Nguyen Sun Jeong Jeon Jens Christian Frisvad Dhanushka N.Wanasinghe Robert Lucking Andre Aptroot Marcela E.S.Caceres Samantha C.Karunarathna Sinang Hongsanan Rungtiwa Phookamsak Nimali Ide Silva Kasun M.Thambugala Ruvishika S.Jayawardena Indunil C.Senanayake Saranyaphat Boonmee Jie Chen Zong-Long Luo Chayanard Phukhamsakda Olinto L.Pereira Vanessa P.Abreu Andre Wilson Campos Rosado Buyck Bart Emile Randrianjohany Vale rie Hofstetter Tatiana B.Gibertoni Adriene Mayrada Silva Soares Helio Longoni Plautz Jr Helen Maria Pontes Sotao William Kalhy Silva Xavier Jadson Diogo Pereira Bezerra Thays Gabrielle Linsde Oliveira Cristina Mariade Souza-Motta Oliane Maria Correia Magalhaes Digvijayini Bundhun Dulanjalee Harishchandra Ishara S.Manawasinghe Wei Dong Sheng-Nan Zhang Dan-Feng Bao Milan C.Samarakoon Dhandevi Pem Anuruddha Karunarathna Chuan-Gen Lin Jing Yang Rekhani H.Perera Vinit Kumar Shi-Ke Huang Monika C.Dayarathne Anusha H.Ekanayaka Subashini C.Jayasiri Yuanpin Xiao Sirinapa Konta Tuula Niskanen Kare Liimatainen Yu-Cheng Dai Xiao-Hong Ji Xue-Mei Tian Armin Mesic Sanjay K.Singh Kunthida Phutthacharoen Lei Cai Touny Sorvongxay Vinodhini Thiyagaraja Chada Norphanphoun Napalai Chaiwan Yong-Zhong Lu Hong-Bo Jiang Jin-Feng Zhang Pranami D.Abeywickrama Janith V.S.Aluthmuhandiram Rashika S.Brahmanage Ming Zeng Thilini Chethana Deping Wei 《Fungal Diversity》 SCIE 2019年第3期1-242,共242页
This article is the tenth series of the Fungal Diversity Notes,where 114 taxa distributed in three phyla,ten classes,30 orders and 53 families are described and illustrated.Taxa described in the present study include ... This article is the tenth series of the Fungal Diversity Notes,where 114 taxa distributed in three phyla,ten classes,30 orders and 53 families are described and illustrated.Taxa described in the present study include one new family(viz.Pseudoberkleasmiaceae in Dothideomycetes),five new genera(Caatingomyces,Cryptoschizotrema,Neoacladium,Paramassaria and Trochilispora)and 71 new species,(viz.Acrogenospora thailandica,Amniculicola aquatica,A.guttulata,Angustimassarina sylvatica,Blackwellomyces lateris,Boubovia gelatinosa,Buellia viridula,Caatingomyces brasiliensis,Calophoma humuli,Camarosporidiella mori,Canalisporium dehongense,Cantharellus brunneopallidus,C.griseotinctus,Castanediella meliponae,Coprinopsis psammophila,Cordyceps succavus,Cortinarius minusculus,C.subscotoides,Diaporthe italiana,D.rumicicola,Diatrypella delonicis,Dictyocheirospora aquadulcis,D.taiwanense,Digitodesmium chiangmaiense,Distoseptispora dehongensis,D.palmarum,Dothiorella styphnolobii,Ellisembia aurea,Falciformispora aquatic,Fomitiporia carpinea,F.lagerstroemiae,Grammothele aurantiaca,G.micropora,Hermatomyces bauhiniae,Jahnula queenslandica,Kamalomyces mangrovei,Lecidella yunnanensis,Micarea squamulosa,Muriphaeosphaeria angustifoliae,Neoacladium indicum,Neodidymelliopsis sambuci,Neosetophoma miscanthi,N.salicis,Nodulosphaeria aquilegiae,N.thalictri,Paramassaria samaneae,Penicillium circulare,P.geumsanense,P.mali-pumilae,P.psychrotrophicum,P.wandoense,Phaeoisaria siamensis,Phaeopoacea asparagicola,Phaeosphaeria penniseti,Plectocarpon galapagoense,Porina sorediata,Pseudoberkleasmium chiangmaiense,Pyrenochaetopsis sinensis,Rhizophydium koreanum,Russula prasina,Sporoschisma chiangraiense,Stigmatomyces chamaemyiae,S.cocksii,S.papei,S.tschirnhausii,S.vikhrevii,Thysanorea uniseptata,Torula breviconidiophora,T.polyseptata,Trochilispora schefflerae and Vaginatispora palmae).Further,twelve new combinations(viz.Cryptoschizotrema cryptotrema,Prolixandromyces australi,P.elongatus,P.falcatus,P.longispinae,P.microveliae,P.neoalardi,P.polhemorum,P.protubera 展开更多
关键词 71 new taxa ASCOMYCOTA BASIDIOMYCOTA DOTHIDEOMYCETES Eurotiomycetes LECANOROMYCETES Leotiomycetes Pezizomycetes Phylogeny Taxonomy
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Characterization of low-N responses in maize(Zea mays L.) cultivars with contrasting nitrogen use efficiency in the North China Plain 被引量:10
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作者 LI Xiang-ling GUO Li-guo +9 位作者 ZHOU Bao-yuan TANG Xiang-ming CHEN Cong-cong ZHANG Lei ZHANG Shao-yun LI Chong-feng XIAO Kai DONG Wei-xin YIN Bao-zhong ZHANG Yue-chen 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2019年第9期2141-2152,共12页
Over-use of N fertilizer in crop production has resulted in a series of environmental problems in the North China Plain(NCP).Thus,improvement of nitrogen use efficiency(NUE)in summer maize has become an effective stra... Over-use of N fertilizer in crop production has resulted in a series of environmental problems in the North China Plain(NCP).Thus,improvement of nitrogen use efficiency(NUE)in summer maize has become an effective strategy for promoting sustainable agriculture in this region.Using twenty maize cultivars,plant dry matter production,N absorption and accumulation,yield formation,and NUE in summer maize were investigated under three N levels in two growing seasons.Based on their yield and yield components,these maize cultivars were categorized into four groups including efficient-efficient(EE)cultivars,high-nitrogen efficient(HNE)cultivars,low-nitrogen efficient(LNE)cultivars and nonefficient-nonefficient(NN)cultivars.In both two seasons,the EE cultivars improved grain yield together with increased plant biomass,and enhanced accumulative amounts as well as higher average grain yields than the other cultivar groups under deficient-N conditions.Significant correlations were observed between yield and kernel numbers(KN),dry matter(DM)amount and N accumulation at both post-silking and maturity stages.DM and N accumulation at late growth stage(i.e.,from silking to maturity)contributed largely to the enhanced yield capacity and improved NUE under N-deficient conditions.Compared with the NN cultivars,the EE cultivars also showed increased N assimilation amount(NAA)and N remobilization content(NRC),and elevated N remobilization efficiency(NRE),NUE and nitrogen partial factor productivity(PFPN).Our investigation has revealed N-associated physiological processes and may provide guidance for cultivation and breeding of high yield and NUE summer maize under limited N conditions in the NCP. 展开更多
关键词 summer MAIZE N deficiency yield dry matter production N ACCUMULATIVE AMOUNT
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A National Key R&D Program: Technologies and Guidelines for Monitoring Ecological Quality of Terrestrial Ecosystems in China 被引量:10
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作者 WANG Shaoqiang WANG Junbang +9 位作者 ZHANG Leiming XIAO Zhishu WANG Feng SUN Nan LI Daiqing CHEN Bin CHEN Jinghua LI Yue WANG Xiaobo WANG Miaomiao 《Journal of Resources and Ecology》 CSCD 2019年第2期105-111,共7页
Ecological quality is defined as the stability, adaptability and resilience of an ecosystem. Monitoring and assessing ecological quality are important bases for China’s ecological civilization construction. The natio... Ecological quality is defined as the stability, adaptability and resilience of an ecosystem. Monitoring and assessing ecological quality are important bases for China’s ecological civilization construction. The national key research and development program "Technologies and guidelines for monitoring ecological quality of terrestrial ecosystems in China", launched in July 2017, includes plans to study the observation technologies and provide guidelines on the ecological in-situ observation, the regional biodiversity and ecosystem function monitoring and its applications, all of which contribute to national ecological quality assessment. A year after its implementation,some important progress has been achieved, such as building the indicator system for comprehensive monitoring of ecological quality and improvement of the methods, mass data transmission, infrared camera-based monitoring of biodiversity, multi-angle automatic spectral observation systems, and unmanned aerial vehicle(UAV) based desert monitoring. We have organized this special issue and attempted to introduce the monitoring techniques and assessment methods on ecological quality from different perspectives in order to further promote the development of ecology and its observation methods. 展开更多
关键词 ecological quality ecological factors BIODIVERSITY ecosystem function monitoring technology monitoring guideli nes
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The ranking of fungi:a tribute to David L.Hawksworth on his 70th birthday 被引量:10
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作者 Kevin D.Hyde Sajeewa S.N.Maharachchikumbura +12 位作者 Sinang Hongsanan Milan C.Samarakoon Robert Lücking Dhandevi Pem Dulanjalee Harishchandra Rajesh Jeewon Rui-Lin Zhao Jian-Chu Xu1 Jian-Chu Xu Jian-Kui Liu Abdullah M.Al-Sadi Ali H.Bahkali Abdallah M.Elgorban 《Fungal Diversity》 SCIE 2017年第3期1-23,共23页
The history of assigning ranks to fungi,as well as the relative importance of using divergence time estimates is reviewed.The paper pays tribute to the major mycological players,and especially to David Hawksworth on h... The history of assigning ranks to fungi,as well as the relative importance of using divergence time estimates is reviewed.The paper pays tribute to the major mycological players,and especially to David Hawksworth on his 70th birthday and his contribution to fungal ranking in Systema Ascomycetum from 1982 to 1998.Following the conclusion of the latter series,the ranking continued with the Outlines of Ascomycota in 2007 and 2010 and more recently with specific classes in‘Towards an outline of Sordariomycetes’and‘Families of Dothideomycetes’.Earlier classifications based on phenotype were certainly more subjective;however,remarkably many of these old arrangements have stood the test of time.More recently,phylogenetic analyses have provided evidence towards a natural classification,resulting in significant changes in many lineages.The classification arrangements however,are still subjective and dependent on the taxa analysed,resulting in different taxonomic interpretations and schemes,particularly when it comes to ranking.Thus,what have been considered as genera by some,have been introduced as families by others.More recently,estimation of divergence times using molecular clock methods have been used as objective evidence for higher ranking of taxa.A divergence period(i.e.200–300 MYA)can be used as a criterion to infer when a group of related taxa evolved and what rank they should be given.We compiled data on divergence times for various higher ranking taxa in the Kingdom Fungi.The kingdom evolved 1000–1600 MYA(Stenian–Calymmian),while the presently accepted phyla evolved between 358 and 541 MYA(Devonian–Cambrian).Divergence times for subphyla are generally between 358 and 485 MYA(Devonian–Ordovician),those of classes 145–358 MYA(Jurassic–Carboniferous),subclasses 66–358 MYA(Cretaceous–Carboniferous),orders 23–252 MYA(Paleogene–Triassic),families 2.8–145 MYA(Neogene–Cretaceous),and genera 2.8–66 MYA(Neogene–Paleogene).Thus,there are wide discrepancies in the times different taxa 展开更多
关键词 Classification Evolution RANKING Temporal banding SORDARIOMYCETES
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Fungal diversity notes 929-1035:taxonomic and phylogenetic contributions on genera and species of fungi 被引量:7
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作者 Rungtiwa Phookamsak Kevin D.Hyde +89 位作者 Rajesh Jeewon D.Jayarama Bhat E.B.Gareth Jones Sajeewa S.N.Maharachchikumbura Olivier Raspe Samantha C.Karunarathna Dhanushka N.Wanasinghe Sinang Hongsanan Mingkwan Doilom Danushka S.Tennakoon Alexandre R.Machado Andre L.Firmino Aniket Ghosh Anuruddha Karunarathna Armin Mesic Arun Kumar Dutta Benjarong Thongbai Bandarupalli Devadatha Chada Norphanphoun Chanokned Senwanna Deping Wei Dhandevi Pem Frank Kwekucher Ackah Gen-Nuo Wang Hong-Bo Jiang Hugo Madrid Hyang Burm Lee Ishani D.Goonasekara Ishara S.Manawasinghe Ivana Kusan Josep Cano Josepa Gene Junfu Li Kanad Das Krishnendu Acharya K.N.Anil Raj K.P.Deepna Latha K.W.Thilini Chethana Mao-Qiang He Margarita Duenas Margita Jadan Maria P.Martin Milan C.Samarakoon Monika C.Dayarathne Mubashar Raza Myung Soo Park M.Teresa Telleria Napalai Chaiwan Neven Matocec Nimali Ide Silva Olinto L.Pereira Paras Nath Singh Patinjareveettil Manimohan Priyanka Uniyal Qiu-Ju Shang Rajendra P.Bhatt Rekhani H.Perera Renato Lucio Mendes Alvarenga Sandra Nogal-Prata Sanjay K.Singh Santhiti Vadthanarat Seung-Yoon Oh Shi-Ke Huang Shiwali Rana Sirinapa Konta Soumitra Paloi Subashini C.Jayasiri Sun Jeong Jeon Tahir Mehmood Tatiana Baptista Gibertoni Thuong T.T.Nguyen Upendra Singh Vinodhini Thiyagaraja V.Venkateswara Sarma Wei Dong Xian-Dong Yu Yong-Zhong Lu Young Woon Lim Yun Chen Zdenko Tkalcec Zhi-Feng Zhang Zong-Long Luo Dinushani A.Daranagama Kasun M.Thambugala Saowaluck Tibpromma Erio Camporesi Timur S.Bulgakov Asha J.Dissanayake Indunil C.Senanayake D 《Fungal Diversity》 SCIE 2019年第2期1-273,共273页
This article is the ninth in the series of Fungal Diversity Notes,where 107 taxa distributed in three phyla,nine classes,31 orders and 57 families are described and illustrated.Taxa described in the present study incl... This article is the ninth in the series of Fungal Diversity Notes,where 107 taxa distributed in three phyla,nine classes,31 orders and 57 families are described and illustrated.Taxa described in the present study include 12 new genera,74 new species,three new combinations,two reference specimens,a re-circumscription of the epitype,and 15 records of sexualasexual morph connections,new hosts and new geographical distributions.Twelve new genera comprise Brunneofusispora,Brunneomurispora,Liua,Lonicericola,Neoeutypella,Paratrimmatostroma,Parazalerion,Proliferophorum,Pseudoastrosphaeriellopsis,Septomelanconiella,Velebitea and Vicosamyces.Seventy-four new species are Agaricus memnonius,A.langensis,Aleurodiscus patagonicus,Amanita flavoalba,A.subtropicana,Amphisphaeria mangrovei,Baorangia major,Bartalinia kunmingensis,Brunneofusispora sinensis,Brunneomurispora lonicerae,Capronia camelliaeyunnanensis,Clavulina thindii,Coniochaeta simbalensis,Conlarium thailandense,Coprinus trigonosporus,Liua muriformis,Cyphellophora filicis,Cytospora ulmicola,Dacrymyces invisibilis,Dictyocheirospora metroxylonis,Distoseptispora thysanolaenae,Emericellopsis koreana,Galiicola baoshanensis,Hygrocybe lucida,Hypoxylon teeravasati,Hyweljonesia indica,Keissleriella caraganae,Lactarius olivaceopallidus,Lactifluus midnapurensis,Lembosia brigadeirensis,Leptosphaeria urticae,Lonicericola hyaloseptispora,Lophiotrema mucilaginosis,Marasmiellus bicoloripes,Marasmius indojasminodorus,Micropeltis phetchaburiensis,Mucor orantomantidis,Murilentithecium lonicerae,Neobambusicola brunnea,Neoeutypella baoshanensis,Neoroussoella heveae,Neosetophoma lonicerae,Ophiobolus malleolus,Parabambusicola thysanolaenae,Paratrimmatostroma kunmingensis,Parazalerion indica,Penicillium dokdoense,Peroneutypa mangrovei,Phaeosphaeria cycadis,Phanerochaete australosanguinea,Plectosphaerella kunmingensis,Plenodomus artemisiae,P.lijiangensis,Proliferophorum thailandicum,Pseudoastrosphaeriellopsis kaveriana,Pseudohelicomyces menglunicus,Pseudoplagiostoma mangiferae,Robillarda mangif 展开更多
关键词 86 new taxa AGARICOMYCETES ASCOMYCOTA BASIDIOMYCOTA Dacrymycetes DOTHIDEOMYCETES Eurotiomycetes LECANOROMYCETES Leotiomycetes Mucoromycetes Mucoromycota Pezizomycetes SORDARIOMYCETES Taxonomy
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Termites and Chinese agricultural system: applications and advances in integrated termite management and chemical control 被引量:8
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作者 Farhan Ahmad Hatem Fouad +2 位作者 Shi-You Liang Yin Hu Jian-Chu Mo 《Insect Science》 SCIE CAS CSCD 2021年第1期2-20,共19页
Termites are eusocial arthropod decomposers,and improve soil fertility,crop yield,and also are used by humans for their ben efits across the world.However,some species of termites are becoming a threat to the farming ... Termites are eusocial arthropod decomposers,and improve soil fertility,crop yield,and also are used by humans for their ben efits across the world.However,some species of termites are becoming a threat to the farming community as they are directly and indirectly causing major losses to the agricultural system.It is estimated that termites cost the global economy more than 40 billion USD annually,and considerable research has been done on their management.In this review,we present the available information related to sustainable and integrated termite management practices(ITM).Furthermore,we insist that the better management of this menace can be possible through:(i)improving traditional methods to keep termites away from crops;(ii)improving agricultural practices to maintain plants with more vigor and less susceptible to termite attack;and(iii)integration of available techniques to reduce termite infestation in crops and surroundings.The application of an effective combination of traditional practices with recently developed approaches is the best option for agricultural growers.Moreover,keeping in mind the beneficial nature of this pest,more innovative efforts for its management,particularly using rapidly emerging technology(e.g.,RNA interference),are needed. 展开更多
关键词 ENTOMOPATHOGENS geographical distribution integrated termite management TERMITE
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Strategy for Use of Rice Blast Resistance Genes in Rice Molecular Breeding 被引量:9
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作者 XIAO Ning WU Yunyu LI Aihong 《Rice science》 SCIE CSCD 2020年第4期263-277,共15页
Rice blast is one of the most destructive diseases affecting rice production worldwide.The development and rational use of resistant varieties has been the most effective and economical measure to control blast.In thi... Rice blast is one of the most destructive diseases affecting rice production worldwide.The development and rational use of resistant varieties has been the most effective and economical measure to control blast.In this review,we summarized the cloning and utilization of rice blast resistance genes,such as Pi1,Pi2,Pi9,Pi54,Pigm and Piz-t.We concluded that three main problems in the current breeding of rice blast resistance are:availability of few R(resistance)genes that confer resistance to both seedling and panicle blast,the resistance effect of pyramided lines is not the result of a simple accumulation of resistance spectrum,and only a few R genes have been successfully used for molecular breeding.Therefore,novel utilization strategies for rice blast R genes in molecular breeding were proposed,such as accurately understanding the utilization of R genes in main modern rice varieties,creating a core resistant germplasm with excellent comprehensive traits,screening and utilizing broadspectrum and durable resistance gene combinations.Lastly,the trends and possible development direction of blast resistance improvement were also discussed,including new genes regulating resistance identified via GWAS(genome-wide association study)and improving rice blast resistance using genetic editing. 展开更多
关键词 rice blast resistance gene molecular breeding strategy
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Evaluation of maize inbred lines currently used in Chinese breeding programs for resistance to six foliar diseases 被引量:8
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作者 Xiaoming Wang Yunhua Zhang +4 位作者 Xiude Xu Hongjie Li Xiaofei Wu Shihuang Zhang Xinhai Li 《The Crop Journal》 SCIE CAS 2014年第4期213-222,共10页
Foliar diseases are common in most maize-producing regions and have caused serious yield reduction in China. To evaluate genetic resistance of parental lines actively used in maize breeding programs to major foliar di... Foliar diseases are common in most maize-producing regions and have caused serious yield reduction in China. To evaluate genetic resistance of parental lines actively used in maize breeding programs to major foliar diseases, 152 maize inbred lines were tested against northern corn leaf blight(NCLB), southern corn leaf blight(SCLB), Curvularia leaf spot(CLS),gray leaf spot(GLS), common rust, and southern rust from 2003 to 2005. A small number of lines exhibited highly resistant reactions to common rust and southern rust, but none were highly resistant to NCLB, SCLB, CLS, and GLS. Although 53.3%, 40.8%, and 80.7% of lines were resistant to NCLB, SCLB, and common rust, the resistance in most lines was moderate.Resistance to CLS, GLS, and southern rust was rare in this collection of maize lines. Five lines,313, Chang 7-2, Qi 319, Qi 318, and Shen 137, were resistant to five diseases tested. Lines belonging to heterotic subgroup PB exhibited better resistance to the foliar diseases than lines from other heterotic subgroups, such as BSSS, PA, Lancaster, LRC, and PA. The results will be of benefit to breeders for selecting lines in disease resistance breeding programs. 展开更多
关键词 ZEA mays SCREENING RESISTANCE FUNGAL DISEASES
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Fungal diversity notes 367-490:taxonomic and phylogenetic contributions to fungal taxa 被引量:6
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作者 Kevin D.Hyde Sinang Hongsanan +88 位作者 Rajesh Jeewon D.Jayarama Bhat Eric H.C.McKenzie E.B.Gareth Jones Rungtiwa Phookamsak Hiran A.Ariyawansa Saranyaphat Boonmee Qi Zhao Faten Awad Abdel-Aziz Mohamed A.Abdel-Wahab Supharat Banmai Putarak Chomnunti Bao-Kai Cui Dinushani A.Daranagama Kanad Das Monika C.Dayarathne Nimali Ide Silva Asha J.Dissanayake Mingkwan Doilom Anusha H.Ekanayaka Tatiana Baptista Gibertoni Aristóteles Góes-Neto Shi-Ke Huang Subashini C.Jayasiri Ruvishika S.Jayawardena Sirinapa Konta Hyang Burm Lee Wen-Jing Li Chuan-Gen Lin Jian-Kui Liu Yong-Zhong Lu Zong-Long Luo Ishara S.Manawasinghe Patinjareveettil Manimohan Ausana Mapook Tuula Niskanen Chada Norphanphoun Moslem Papizadeh Rekhani H.Perera Chayanard Phukhamsakda Christian Richter AndréL.C.Mde A.Santiago E.Ricardo Drechsler-Santos Indunil C.Senanayake Kazuaki Tanaka T.M.D.S.Tennakoon Kasun M.Thambugala Qing Tian Saowaluck Tibpromma Benjarong Thongbai Alfredo Vizzini Dhanushka N.Wanasinghe Nalin N.Wijayawardene Hai-Xia Wu Jing Yang Xiang-Yu Zeng Huang Zhang Jin-Feng Zhang Timur S.Bulgakov Erio Camporesi Ali H.Bahkali Mohammad A.Amoozegar Lidia Silva Araujo-Neta Joseph F.Ammirati Abhishek Baghela R.P.Bhatt Dimitar Bojantchev Bart Buyck Gladstone Alves da Silva Catarina Letícia Ferreira de Lima Rafael JoséVilela de Oliveira Carlos Alberto Fragoso de Souza Yu-Cheng Dai Bálint Dima Tham Thi Duong Enrico Ercole Fernando Mafalda-Freire Aniket Ghosh Akira Hashimoto Sutakorn Kamolhan Ji-Chuan Kang Samantha C.Karunarathna Paul M.Kirk Ilkka Kytovuori Angela Lantieri Kare Liimatainen Zuo-Yi Liu Xing-Zhong Liu Robert Lückin 《Fungal Diversity》 SCIE 2016年第5期1-270,共270页
This is a continuity of a series of taxonomic papers where materials are examined,described and novel combinations are proposed where necessary to improve our traditional species concepts and provide updates on their ... This is a continuity of a series of taxonomic papers where materials are examined,described and novel combinations are proposed where necessary to improve our traditional species concepts and provide updates on their classification.In addition to extensive morphological descriptions and appropriate asexual and sexual connections,DNA sequence data are also analysed from concatenated datasets(rDNA,TEF-a,RBP2 and b-Tubulin)to infer phylogenetic relationships and substantiate systematic position of taxa within appropriate ranks.Wherever new species or combinations are being proposed,we apply an integrative approach(morphological and molecular data as well as ecological features wherever applicable).Notes on 125 fungal taxa are compiled in this paper,including eight new genera,101 new species,two new combinations,one neotype,four reference specimens,new host or distribution records for eight species and one alternative morphs.The new genera introduced in this paper are Alloarthopyrenia,Arundellina,Camarosporioides,Neomassaria,Neomassarina,Neotruncatella,Paracapsulospora and Pseudophaeosphaeria.The new species are Alfaria spartii,Alloarthopyrenia italica,Anthostomella ravenna,An.thailandica,Arthrinium paraphaeospermum,Arundellina typhae,Aspergillus koreanus,Asterina cynometrae,Bertiella ellipsoidea,Blastophorum aquaticum,Cainia globosa,Camarosporioides phragmitis,Ceramothyrium menglunense,Chaetosphaeronema achilleae,Chlamydotubeufia helicospora,Ciliochorella phanericola,Clavulinopsis aurantiaca,Colletotrichum insertae,Comoclathris italica,Coronophora myricoides,Cortinarius fulvescentoideus,Co.nymphatus,Co.pseudobulliardioides,Co.tenuifulvescens,Cunninghamella gigacellularis,Cyathus pyristriatus,Cytospora cotini,Dematiopleospora alliariae,De.cirsii,Diaporthe aseana,Di.garethjonesii,Distoseptispora multiseptata,Dis.tectonae,Dis.tectonigena,Dothiora buxi,Emericellopsis persica,Gloniopsis calami,Helicoma guttulatum,Helvella floriforma,H.oblongispora,Hermatomyces subiculosa,Juncaceicola italica,Lactarius dirkii,Lentithecium 展开更多
关键词 ASCOMYCOTA BASIDIOMYCOTA DOTHIDEOMYCETES Mortierellomycotina Mucoromycotina Pezizomycetes Phylogeny Taxonomy New genus New species New host records
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Expression of an(E)-β-farnesene synthase gene from Asian peppermint in tobacco affected aphid infestation 被引量:7
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作者 Xiudao Yu Yongjun Zhang +3 位作者 Youzhi Ma Zhaoshi Xu Genping Wang Lanqin Xia 《The Crop Journal》 SCIE CAS 2013年第1期50-60,共11页
Aphids are major agricultural pests that cause significant yield losses in crop plants each year.(E)-β-farnesene(EβF) is the main or only component of an alarm pheromone involved in chemical communication within aph... Aphids are major agricultural pests that cause significant yield losses in crop plants each year.(E)-β-farnesene(EβF) is the main or only component of an alarm pheromone involved in chemical communication within aphid species and particularly in the avoidance of predation. EβF also occurs in the essential oil of some plant species, and is catalyzed by EβF synthase. By using oligonucleotide primers designed from the known sequence of an EβF synthase gene from black peppermint(Mentha × piperita), two cDNA sequences, MaβFS1 and MaβFS2, were isolated from Asian peppermint(Mentha asiatica). Expression pattern analysis showed that the MaβFS1 gene exhibited higher expression in flowers than in roots, stems and leaves at the transcriptional level. Overexpression of MaβFS1 in tobacco plants resulted in emission of pure EβF ranging from 2.62 to 4.85 ng d-1g-1of fresh tissue. Tritrophic interactions involving peach aphids(Myzus persicae), and predatory lacewing(Chrysopa septempunctata) larvae demonstrated that transgenic tobacco expressing MaβFS1 had lower aphid infestation. This result suggested that the EβF synthase gene from Asian peppermint could be a good candidate for genetic engineering of agriculturally important crop plants. 展开更多
关键词 EβF synthase gene MENTHA asiatica MYZUS persicae NICOTIANA tabacum
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Development and identification of two novel wheat-rye 6R derivative lines with adult-plant resistance to powdery mildew and high-yielding potential 被引量:1
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作者 Guohao Han Jing Wang +10 位作者 Hanwen Yan Tiantian Gu Lijun Cao Shiyu Liu Xiuquan Li Yilin Zhou Jieru Fan Zhipeng Shi Hong Liu Lihui Li Diaoguo An 《The Crop Journal》 SCIE CSCD 2024年第1期308-313,共6页
Powdery mildew,caused by Blumeria graminis f.sp.tritici(Bgt),is a devastating disease that seriously threatens wheat yield and quality.To control this disease,host resistance is the most effective measure.Compared wit... Powdery mildew,caused by Blumeria graminis f.sp.tritici(Bgt),is a devastating disease that seriously threatens wheat yield and quality.To control this disease,host resistance is the most effective measure.Compared with the resistance genes from common wheat,alien resistance genes can better withstand infection of this highly variable pathogen.Development of elite alien germplasm resources with powdery mildew resistance and other key breeding traits is an attractive strategy in wheat breeding.In this study,three wheat-rye germplasm lines YT4-1,YT4-2,and YT4-3 were developed through hybridization between octoploid triticale and common wheat,out of which the lines YT4-1 and YT4-2 conferred adult-plant resistance(APR)to powdery mildew while the line YT4-3 was susceptible to powdery mildew during all of its growth stages.Using genomic in situ hybridization,multi-color fluorescence in situ hybridization,multi-color GISH,and molecular marker analysis,YT4-1,YT4-2,and YT4-3 were shown to be cytogenetically stable wheat-rye 6R addition and T1RS.1BL translocation line,6RL ditelosomic addition and T1RS.1BL translocation line,and T1RS.1BL translocation line,respectively.Compared with previously reported wheat-rye derivative lines carrying chromosome 6R,YT4-1 and YT4-2 showed stable APR without undesirable pleiotropic effects on agronomic traits.Therefore,these novel wheat-rye 6R derivative lines are expected to be promising bridge resources in wheat disease breeding. 展开更多
关键词 Powdery mildew Secale cereale Triticum aestivum Wheat-rye 6R derivatives Agronomic performance
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Bemisia tabaci Phylogenetic Groups in India and the Relative Transmission Efficacy of Tomato leaf curl Bangalore virus by an Indigenous and an Exotic Population 被引量:8
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作者 R V Chowda-Reddy M Kirankumar +4 位作者 Susan E Seal V Muniyappa Girish B Valand M R Govindappa John Colvin 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2012年第2期235-248,共14页
Bemisia tabaci adults from various host-plant species were collected from 31 regions across India.266 B.tabaci samples were first screened by RAPD-PCR to examine molecular variability and to select individuals with di... Bemisia tabaci adults from various host-plant species were collected from 31 regions across India.266 B.tabaci samples were first screened by RAPD-PCR to examine molecular variability and to select individuals with different fingerprints.Host-plant and region of collection were then used to select 25 individuals for PCR amplification and sequencing of their partial mitochondrial cytochrome oxidase subunit one(mtCOI)genes.Pairwise comparisons with mtCOI consensus sequences showed that the majority of these samples had 〈3.5% sequence divergence from groups currently termed Asia I,Asia II-5,Asia II-7,and Asia II-8.The biotype-B B.tabaci from India clustered into the Middle East-Asia Minor 1 group.A new group of B.tabaci from Coimbatore,collected from pumpkin,was related most closely to the Asia I group(6.2% sequence divergence from the consensus Asia I sequence).To increase our understanding of the epidemiology of tomato leaf curl disease(ToLCD)and the key B.tabaci genetic groups involved in virus spread,the indigenous Asia I and the exotic biotype-B population from South India were used to carry out transmission experiments using Tomato leaf curl Bangalore virus(ToLCBV).The acquisition access periods(AAP),inoculation access periods(IAP),latent periods(LP),and ToLCBV transmission efficiencies of the two populations were compared and the biotype-B had the more efficient transmission characteristics.These results are discussed in relation to recent changes in the epidemiology of tomato leaf curl disease in South India. 展开更多
关键词 BEGOMOVIRUSES mitochondrial cytochrome oxidase WHITEFLY Bemisia tabaci virus-vector relationships epidemiology
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Genomic analysis,trajectory tracking,and field surveys reveal sources and long-distance dispersal routes of wheat stripe rust pathogen in China 被引量:1
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作者 Yuxiang Li Jichen Dai +24 位作者 Taixue Zhang Baotong Wang Siyue Zhang Conghao Wang Jiguang Zhang Qiang Yao Mingju Li Chengyun Li Yuelin Peng Shiqin Cao Gangming Zhan Fei Tao Haifeng Gao Weili Huang Xiaojun Feng Yingwen Bai Zhuoma Qucuo Hongsheng Shang Chong Huang Wancai Liu Jiasui Zhan Xiangming Xu Xianming Chen Zhensheng Kang Xiaoping Hu 《Plant Communications》 SCIE CSCD 2023年第4期174-188,共15页
Identifying sources of phytopathogen inoculum and determining their contributions to disease outbreaks are essential for predicting disease development and establishing control strategies.Puccinia striiformis f.sp.tri... Identifying sources of phytopathogen inoculum and determining their contributions to disease outbreaks are essential for predicting disease development and establishing control strategies.Puccinia striiformis f.sp.tritici(Pst),the causal agent of wheat stripe rust,is an airborne fungal pathogen with rapid virulence variation that threatens wheat production through its long-distance migration.Because of wide variation in geographic features,climatic conditions,and wheat production systems,Pst sources and related dispersal routes in China are largely unclear.In the present study,we performed genomic analyses of 154 Pst isolates from all major wheat-growing regions in China to determine Pst population structure and diversity.Through trajectory tracking,historical migration studies,genetic introgression analyses,and field surveys,we investigated Pst sources and their contributions to wheat stripe rust epidemics.We identified Longnan,the Himalayan region,and the Guizhou Plateau,which contain the highest population genetic diversities,as the Pst sources in China.Pst from Longnan disseminates mainly to eastern Liupan Mountain,the Sichuan Basin,and eastern Qinghai;that from the Himalayan region spreads mainly to the Sichuan Basin and eastern Qinghai;and that from the Guizhou Plateau migrates mainly to the Sichuan Basin and the Central Plain.These findings improve our current understanding of wheat stripe rust epidemics in China and emphasize the need for managing stripe rust on a national scale. 展开更多
关键词 Puccinia striiformis f.sp.tritici stripe rust disease epidemics population genetics genome sequencing
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Genetic Transformation of Rice with Pi-d2 Gene Enhances Resistance to Rice Blast Fungus Magnaporthe oryzae 被引量:7
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作者 CHEN De-xi CHEN Xue-wei +3 位作者 MA Bing-tian WANG Yu-ping ZHU Li-huang LI Shi-gui 《Rice science》 SCIE 2010年第1期19-27,共9页
The gene Pi-d2, conferring gene-for-gene resistance to the Chinese blast strain ZB15, was isolated from a rice variety (Digu) by the map-based cloning strategy. Here, we constructed a control plasmid pZH01-pi-d2tp3... The gene Pi-d2, conferring gene-for-gene resistance to the Chinese blast strain ZB15, was isolated from a rice variety (Digu) by the map-based cloning strategy. Here, we constructed a control plasmid pZH01-pi-d2tp309 (pZH01-tp309) and three different expression constructs, pCB-Pi-d25.3kb (pCB5.3kb), pCB-Pi-d26.3kb (pCB6.3kb) and pZH01-Pi-d22.72kb (pZH01-2.72kb) of Pi-d2, driven by Pi-d2 gene’s own promoter or CaMV35S promoter. These constructs were separately introduced into japonica rice varieties Lijiangxintuanhegu, Taipei 309, Nipponbare and Zhonghua 9 through Agrobacterium- mediated transformation. A total of 150 transgenic rice plants were obtained from the regenerated calli selected on hygromycin. PCR, RT-PCR and Southern-blotting assay showed that the gene of interest had been integrated into rice genome and stably inherited. Thirty-five transgenic lines independently derived from T1 progeny were inoculated with the rice blast strain ZB15. Transformants exhibited resistance to rice blast at various levels. The lesions on the transgenic plant leaves were less severe than those on the controls and the resistance level of transgenic plants harboring the gene of interest from three vectors had no difference. The own promoter of Pi-d2, about 2.2 kb or 3.2 kb, had the similar promoter function as CaMV35S. Field evaluation for three successive years supported the results of artificial trial, and some lines with high resistance to rice leaf blast and neck blast were obtained. 展开更多
关键词 rice resistance gene rice blast fungus gene transfer
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Adenine base editor incorporating the N-methylpurine DNA glycosylase MPGv3 enables efficient A-to-K base editing in rice 被引量:2
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作者 Xuemei Wu Bin Ren +8 位作者 Lang Liu Shengqun Qiu Xin’ge Li Peijing Li Fang Yan Honghui Lin Xueping Zhou Dawei Zhang Huanbin Zhou 《Plant Communications》 SCIE CSCD 2023年第6期230-233,共4页
Dear Editor,Crop genetic diversity and elite agronomic traits are mainly caused by genetic variants,approximately half of which are single-nucleotide polymorphisms.Precise nucleotide substitution through CRISPR–Cas-m... Dear Editor,Crop genetic diversity and elite agronomic traits are mainly caused by genetic variants,approximately half of which are single-nucleotide polymorphisms.Precise nucleotide substitution through CRISPR–Cas-mediated base editors has been widely used to correct defective alleles and create novel alleles by artificial evolution for rapid crop genetic improvement.Since 2017,cytosine base editors(CBEs)and adenine base editors(ABEs)have been successively developed in many plant species and have been continuously optimized to generate highly efficient C-to-T and A-to-G transitions,as well as by-product C-to-A/G conversion with low efficiency(Ren et al.,2018;Yan et al.,2021). 展开更多
关键词 ELITE BASE EDITOR
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